Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00075  |
| T6SS type | Type i1 |
| Strain | Burkholderia pseudomallei K96243 |
| Replicon (RefSeq) | chromosome II (NC_006351) |
| Location | 2036034..2064669 |
| Function | anti-eukaryotic; effector translocation; virulence |
| Comment | Virulence in Syrian hamster model; growth and mononuclear giant cell formation in cultured macrophages; intercellular spread in cultured cells. |
| References | [1] Chen Y et al (2011) Regulation of type VI secretion system during Burkholderia pseudomallei infection. Infect Immun. 79(8):3064-73. [PudMed:21670170] [2] Hopf V et al (2014) BPSS1504, a cluster 1 type VI secretion gene, is involved in intracellular survival and virulence of Burkholderia pseudomallei. Infect Immun. 82(5):2006-15. [PudMed:24595140] [3] Chen Y et al (2014) Characterization and analysis of the Burkholderia pseudomallei BsaN virulence regulon. BMC Microbiol. 14(1):206. [PudMed:25085508] [4] Burtnick MN et al (2011) The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomallei. Infect Immun. 79(4):1512-25. [PudMed:21300775] [5] Fisher NA et al (2012) The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 0.58125. [PudMed:22892068] [6] Chirakul S et al (2014) Characterization of BPSS1521 (bprD), a Regulator of Burkholderia pseudomallei Virulence Gene Expression in the Mouse Model. PLoS One. 9(8):e104313. [PudMed:25111708] [7] Shalom G et al (2007) In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages. Microbiology. 153(Pt 8):2689-99. [PudMed:17660433] [8] Toesca IJ et al (2014) The Type VI Secretion System Spike Protein VgrG5 Mediates Membrane Fusion during Intercellular Spread by Pseudomallei Group Burkholderia Species. Infect Immun. 82(4):1436-44. [PudMed:24421040] [9] Sun GW et al (2010) Identification of a regulatory cascade controlling Type III Secretion System 3 gene expression in Burkholderia pseudomallei. Mol Microbiol. 76(3):677-89. [PudMed:20345664] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM | VirA | VirG |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| BPS_RS26850 | 2031208..2032233 | + | 1026 | WP_004206324.1 | N-acetylmuramoyl-L-alanine amidase | ||
| BPS_RS26855 | 2032361..2033761 | + | 1401 | WP_004525349.1 | autotransporter strand-loop-strand O-heptosyltransferase | ||
| BPS_RS32200 (bimA) | 2033784..2035334 | + | 1551 | WP_011205626.1 | actin-based motility protein BimA | ||
| BPS_RS26865 | 2035414..2035791 | + | 378 | WP_004553514.1 | hypothetical protein | ||
| T6CP008439 | TssA | BPS_RS26870 (tssA) | 2036034..2037986 | - | 1953 | WP_045606108.1 | type VI secretion system protein TssA |
| T6CP012441 | VirG | BPS_RS26875 | 2037977..2038714 | - | 738 | WP_004528788.1 | response regulator VirG |
| T6CP012442 | VirA | BPS_RS26880 | 2038711..2040555 | - | 1845 | WP_011205628.1 | two-component system sensor kinase |
| T6CP000664 | TssB | BPS_RS26885 (tssB) | 2040815..2041309 | + | 495 | WP_004530571.1 | type VI secretion system contractile sheath small subunit |
| T6CP000665 | TssC | BPS_RS26890 (tssC) | 2041306..2042832 | + | 1527 | WP_004200974.1 | type VI secretion system contractile sheath large subunit |
| T6CP008440 | TssD | BPS_RS26895 (hcp1) | 2043052..2043561 | + | 510 | WP_004525344.1 | type VI secretion system effector Hcp1 |
| T6CP008441 | TssE | BPS_RS26900 (tssE) | 2043554..2044015 | + | 462 | WP_004530572.1 | type VI secretion system baseplate subunit TssE |
| T6CP000666 | TssF | BPS_RS26905 (tssF) | 2044052..2045794 | + | 1743 | WP_004551874.1 | type VI secretion system baseplate subunit TssF |
| T6CP000667 | TssG | BPS_RS26910 (tssG) | 2045782..2046804 | + | 1023 | WP_004533373.1 | type VI secretion system baseplate subunit TssG |
| T6CP000668 | TssH | BPS_RS26915 (tssH) | 2046791..2049829 | + | 3039 | WP_011205629.1 | ATP-dependent protease ClpV |
| T6CP000669 | TssI | BPS_RS26920 (tssI) | 2049856..2052879 | + | 3024 | WP_009940775.1 | type VI secretion system tip protein VgrG |
| BPS_RS26925 | 2052905..2055547 | + | 2643 | WP_011205630.1 | cluster 1 type VI secretion system protein TagAB-5 | ||
| BPS_RS26930 | 2055565..2056629 | + | 1065 | WP_004556233.1 | type VI secretion system-associated protein TagB-5 | ||
| BPS_RS26935 | 2056626..2057378 | + | 753 | WP_004551877.1 | type VI secretion system-associated protein TagC-5 | ||
| BPS_RS26940 (tssI) | 2057407..2057799 | + | 393 | WP_004200966.1 | type VI secretion system protein TssI | ||
| T6CP009348 | TssJ | BPS_RS26945 (tssJ) | 2057809..2058606 | + | 798 | WP_004533362.1 | type VI secretion system lipoprotein TssJ |
| T6CP000670 | TssK | BPS_RS26950 (tssK) | 2058639..2060036 | + | 1398 | WP_004536933.1 | type VI secretion system baseplate subunit TssK |
| T6CP000671 | TssL | BPS_RS26955 (tssL) | 2060033..2060698 | + | 666 | WP_004551879.1 | type VI secretion system protein TssL |
| T6CP000672 | TssM | BPS_RS26960 (tssM-5) | 2060695..2064669 | + | 3975 | WP_181937968.1 | type VI secretion system membrane subunit TssM-5 |
| BPS_RS26965 | 2065386..2066609 | + | 1224 | WP_160447233.1 | Ig-like domain-containing protein | ||
| BPS_RS26970 | 2066569..2066814 | - | 246 | WP_004536825.1 | hypothetical protein | ||
| BPS_RS32205 (tssN) | 2066773..2067066 | + | 294 | WP_009940791.1 | type VI secretion system protein TssN | ||
| BPS_RS26975 | 2067071..2067703 | + | 633 | WP_009968906.1 | GTP cyclohydrolase I | ||
| BPS_RS26980 | 2067796..2068103 | + | 308 | pseudogene | DDE-type integrase/transposase/recombinase |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF00094 |
BPS_RS26895 | - | - | Burkholderia pseudomallei K96243 | chromosome (NC_006351) | 2043052..2043561 (+) | 53722520 |
| EFF00092 |
BPS_RS25320 | - | - | Burkholderia pseudomallei K96243 | chromosome (NC_006351) | 1633710..1635950 (-) | 53722235 |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
|---|