Detailed information of component protein


Summary

Component ID T6CP000671
Component protein type TssL
T6SS ID (Type) T6SS00075 experimental (Type i1)
Strain Burkholderia pseudomallei K96243
Replicon chromosome II
Sequence Protein sequence (222 a.a.); Nucleotide sequence (666 bp)
Reference
[1] Burtnick MN, Brett PJ, Harding SV, et al. The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomallei. Infect Immun. 2011 Apr;79(4):1512-25. doi: 10.1128/IAI.01218-10. Epub 2011 Feb 7. PMID: 21300775
[2] Shalom G, Shaw JG, Thomas MS. In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages. Microbiology (Reading). 2007 Aug;153(Pt 8):2689-2699. doi: 10.1099/mic.0.2007/006585-0. PMID: 17660433
[3] Chen Y, Wong J, Sun GW, et al. Regulation of type VI secretion system during Burkholderia pseudomallei infection. Infect Immun. 2011 Aug;79(8):3064-73. doi: 10.1128/IAI.05148-11. Epub 2011 Jun 13. PMID: 21670170
[4] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
[5] Sun GW, Chen Y, Liu Y, et al. Identification of a regulatory cascade controlling Type III Secretion System 3 gene expression in Burkholderia pseudomallei. Mol Microbiol. 2010 May;76(3):677-89. doi: 10.1111/j.1365-2958.2010.07124.x. Epub 2010 Mar 24. PMID: 20345664
[6] Toesca IJ, French CT, Miller JF. The Type VI secretion system spike protein VgrG5 mediates membrane fusion during intercellular spread by pseudomallei group Burkholderia species. Infect Immun. 2014 Apr;82(4):1436-44. doi: 10.1128/IAI.01367-13. Epub 2014 Jan 13. PMID: 24421040
[7] Hopf V, Göhler A, Eske-Pogodda K, et al. BPSS1504, a cluster 1 type VI secretion gene, is involved in intracellular survival and virulence of Burkholderia pseudomallei. Infect Immun. 2014 May;82(5):2006-15. doi: 10.1128/IAI.01544-14. Epub 2014 Mar 4. PMID: 24595140
[8] Chen Y, Schröder I, French CT, et al. Characterization and analysis of the Burkholderia pseudomallei BsaN virulence regulon. BMC Microbiol. 2014 Aug 1;14:206. doi: 10.1186/s12866-014-0206-6. PMID: 25085508
[9] Chirakul S, Bartpho T, Wongsurawat T, et al. Characterization of BPSS1521 (bprD), a regulator of Burkholderia pseudomallei virulence gene expression in the mouse model. PLoS One. 2014 Aug 11;9(8):e104313. doi: 10.1371/journal.pone.0104313. eCollection 2014. PMID: 25111708
[10] Chen Y, Schröder I, French CT, et al. Characterization and analysis of the Burkholderia pseudomallei BsaN virulence regulon. BMC Microbiol. 2014 Aug 1;14:206. doi: 10.1186/s12866-014-0206-6. PMID: 25085508
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BPS_RS26955
Coordinate (Strand) 2060033..2060698 (+)
NCBI ID WP_004551879.1
RefSeq NC_006351
Uniprot ID UPI00025C21A6
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 2.4e-43 3..207
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-186 MKLTDSMMPLVAYARQLAQSPRGDAAEVALRLDILIERARDHAACAGAPAADVDDALFAVCAWIDEALLNSGWDDADRWTLRLLQKRYFDTSHAGVVFFERLGTLDGARASVLEVYLLCLRLGFRGRYGYDGGDGPLDAIRQRALDALSAHAPPCAAGEPPFPAAYAHAGPHAAAAKARTRRRIAR
Transmembrane helix 187-209 AGLSVGLPLAVLLSLYLIYHVVI
Outside 210-221 VRMVDAVYPRLQ


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9492 - -


  Protein sequence: 222 a.a.    

>T6CP000671 NC_006351:2060033-2060698 [Burkholderia pseudomallei K96243] [TssL]
MKLTDSMMPLVAYARQLAQSPRGDAAEVALRLDILIERARDHAACAGAPAADVDDALFAVCAWIDEALLNSGWDDADRWT
LRLLQKRYFDTSHAGVVFFERLGTLDGARASVLEVYLLCLRLGFRGRYGYDGGDGPLDAIRQRALDALSAHAPPCAAGEP
PFPAAYAHAGPHAAAAKARTRRRIARAGLSVGLPLAVLLSLYLIYHVVIVRMVDAVYPRLQ*

  Nucleotide sequence: 666 bp    

>T6CP000671 NC_006351:2060033-2060698 [Burkholderia pseudomallei K96243] [TssL]
ATGAAGCTGACCGATTCGATGATGCCGCTCGTCGCGTATGCGCGGCAGCTCGCGCAATCGCCGCGCGGCGACGCCGCCGA
AGTCGCGCTGCGGCTCGACATCCTGATCGAGCGCGCGCGCGACCACGCGGCGTGCGCGGGCGCACCCGCGGCCGACGTCG
ACGACGCGCTGTTCGCTGTGTGCGCGTGGATCGACGAGGCGCTGCTGAACAGCGGCTGGGACGATGCCGATCGCTGGACG
CTGCGGCTGCTGCAAAAGCGCTACTTCGATACGTCGCACGCGGGCGTCGTGTTCTTCGAACGCCTCGGCACGCTCGACGG
CGCGCGCGCGAGCGTGCTCGAGGTCTACCTGCTGTGCCTGCGGCTCGGCTTTCGCGGCCGCTACGGCTACGACGGCGGCG
ACGGCCCGCTCGACGCGATCCGGCAGCGCGCGCTCGATGCACTGTCGGCGCACGCGCCGCCGTGCGCCGCGGGCGAGCCG
CCGTTTCCCGCCGCCTATGCGCACGCCGGCCCGCACGCCGCCGCCGCGAAAGCGCGCACGCGCCGCCGGATCGCGCGCGC
GGGGCTGAGCGTCGGGCTGCCGCTCGCCGTCCTGCTGTCGCTCTATCTGATCTATCACGTCGTCATCGTGCGGATGGTCG
ACGCGGTGTACCCCAGGCTGCAATGA