Detailed information of component protein


Summary

Component ID T6CP000668
Component protein type TssH
T6SS ID (Type) T6SS00075 experimental (Type i1)
Strain Burkholderia pseudomallei K96243
Replicon chromosome II
Sequence Protein sequence (1013 a.a.); Nucleotide sequence (3039 bp)
Reference
[1] Burtnick MN, Brett PJ, Harding SV, et al. The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomallei. Infect Immun. 2011 Apr;79(4):1512-25. doi: 10.1128/IAI.01218-10. Epub 2011 Feb 7. PMID: 21300775
[2] Shalom G, Shaw JG, Thomas MS. In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages. Microbiology (Reading). 2007 Aug;153(Pt 8):2689-2699. doi: 10.1099/mic.0.2007/006585-0. PMID: 17660433
[3] Chen Y, Wong J, Sun GW, et al. Regulation of type VI secretion system during Burkholderia pseudomallei infection. Infect Immun. 2011 Aug;79(8):3064-73. doi: 10.1128/IAI.05148-11. Epub 2011 Jun 13. PMID: 21670170
[4] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
[5] Sun GW, Chen Y, Liu Y, et al. Identification of a regulatory cascade controlling Type III Secretion System 3 gene expression in Burkholderia pseudomallei. Mol Microbiol. 2010 May;76(3):677-89. doi: 10.1111/j.1365-2958.2010.07124.x. Epub 2010 Mar 24. PMID: 20345664
[6] Toesca IJ, French CT, Miller JF. The Type VI secretion system spike protein VgrG5 mediates membrane fusion during intercellular spread by pseudomallei group Burkholderia species. Infect Immun. 2014 Apr;82(4):1436-44. doi: 10.1128/IAI.01367-13. Epub 2014 Jan 13. PMID: 24421040
[7] Hopf V, Göhler A, Eske-Pogodda K, et al. BPSS1504, a cluster 1 type VI secretion gene, is involved in intracellular survival and virulence of Burkholderia pseudomallei. Infect Immun. 2014 May;82(5):2006-15. doi: 10.1128/IAI.01544-14. Epub 2014 Mar 4. PMID: 24595140
[8] Chen Y, Schröder I, French CT, et al. Characterization and analysis of the Burkholderia pseudomallei BsaN virulence regulon. BMC Microbiol. 2014 Aug 1;14:206. doi: 10.1186/s12866-014-0206-6. PMID: 25085508
[9] Chirakul S, Bartpho T, Wongsurawat T, et al. Characterization of BPSS1521 (bprD), a regulator of Burkholderia pseudomallei virulence gene expression in the mouse model. PLoS One. 2014 Aug 11;9(8):e104313. doi: 10.1371/journal.pone.0104313. eCollection 2014. PMID: 25111708
[10] Chen Y, Schröder I, French CT, et al. Characterization and analysis of the Burkholderia pseudomallei BsaN virulence regulon. BMC Microbiol. 2014 Aug 1;14:206. doi: 10.1186/s12866-014-0206-6. PMID: 25085508
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BPS_RS26915
Coordinate (Strand) 2046791..2049829 (+)
NCBI ID WP_011205629.1
RefSeq NC_006351
Uniprot ID Q63K63_BURPS
KEGG ID bps:BPSS1502
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 7.6e-41 685..852
AAA_lid_9 PF17871.3 4.9e-33 365..456
ClpB_D2-small PF10431.11 5.1e-12 857..926
AAA PF00004.31 5.1e-09 227..336
Clp_N PF02861.22 3.5e-05 28..75
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-1012 MLLVDLKPLIEQLNPYCRNALESAVGACVARRHDDVAVEHLLARLCDEPSADVALLLRACGADAARLRRQADAALDARPAGDGGRPAFAPSLLALLQDAWLIASLELGDTHIRSAAVLAAAVARAARQPSPGGDDVLQSLRKDALVARFASGACARSIESRASGALDAAPGEASDTRDASSAIARYCEDFTAKARAGGIDPVFGRDAEIRQIVDILARRRKNNPVCVGEPGVGKTAVVEGLALRIAEGDVPATLRGATLLGLDLGMLQAGASVKGEFEQRLKRVIAEIRASQTPVVLFIDEAHTLIGAGGAAGASDAANLLKPALARGELRTIAATTWSEYKKYFEKDAALARRFQPVKLDSPNVATSVMILRGLKERYQDAHGVTIRDDALVAAAELSARYITGRQLPDKAIDLLDTACARVKVRQQTKPAALEDAQRAIQALERERRALRDELAERCAPDTPRVADIDRELAALSARAGALRDAWAAQRDAAQALVDARRACRAAADATDAAGTTGTAHAGELADSADVSDIADVAKMADAADAAPADAETGSNARDADVRMPAGTCARIAPNPDARARPPLRAPHADARERAARALAEATRRFEHAQRDTPLVRIDVDPDAIADVVADWTGIAAGKLRRDRANVMLRLADTLRRRIRGQDHAIEQISEAVKAGAAGVHDPRRPLGVFLLAGPSGTGKTETALAVADALFGDERSIVVVNMSEFQERHDVSRLIGSPPGYVGYGEGGMLTEAVRQRPYSVVLLDEVEKAHPDVLNLFYQVFDKGSLSDGEGKEVDFANTVIFLTSNLGADIIADIAARGARPDPDAMRAAVRPALSRHFKPALLARMTEIPYAPLAPDTLADIARLKLERIAARVAAQHATRIVYDDAVVAHVAARCTEVESGARNVDFILQRHVLPALAQHVLVCAGNAAPMPAIRVAIDAGGRFVVSNDAPADSDARDRSDASDASDASDAIDAVDAVDAVDAPNAPNAPNAPNAPNAPNAPNAPNAR


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9883 - -


  Protein sequence: 1013 a.a.    

>T6CP000668 NC_006351:2046791-2049829 [Burkholderia pseudomallei K96243] [TssH]
MLLVDLKPLIEQLNPYCRNALESAVGACVARRHDDVAVEHLLARLCDEPSADVALLLRACGADAARLRRQADAALDARPA
GDGGRPAFAPSLLALLQDAWLIASLELGDTHIRSAAVLAAAVARAARQPSPGGDDVLQSLRKDALVARFASGACARSIES
RASGALDAAPGEASDTRDASSAIARYCEDFTAKARAGGIDPVFGRDAEIRQIVDILARRRKNNPVCVGEPGVGKTAVVEG
LALRIAEGDVPATLRGATLLGLDLGMLQAGASVKGEFEQRLKRVIAEIRASQTPVVLFIDEAHTLIGAGGAAGASDAANL
LKPALARGELRTIAATTWSEYKKYFEKDAALARRFQPVKLDSPNVATSVMILRGLKERYQDAHGVTIRDDALVAAAELSA
RYITGRQLPDKAIDLLDTACARVKVRQQTKPAALEDAQRAIQALERERRALRDELAERCAPDTPRVADIDRELAALSARA
GALRDAWAAQRDAAQALVDARRACRAAADATDAAGTTGTAHAGELADSADVSDIADVAKMADAADAAPADAETGSNARDA
DVRMPAGTCARIAPNPDARARPPLRAPHADARERAARALAEATRRFEHAQRDTPLVRIDVDPDAIADVVADWTGIAAGKL
RRDRANVMLRLADTLRRRIRGQDHAIEQISEAVKAGAAGVHDPRRPLGVFLLAGPSGTGKTETALAVADALFGDERSIVV
VNMSEFQERHDVSRLIGSPPGYVGYGEGGMLTEAVRQRPYSVVLLDEVEKAHPDVLNLFYQVFDKGSLSDGEGKEVDFAN
TVIFLTSNLGADIIADIAARGARPDPDAMRAAVRPALSRHFKPALLARMTEIPYAPLAPDTLADIARLKLERIAARVAAQ
HATRIVYDDAVVAHVAARCTEVESGARNVDFILQRHVLPALAQHVLVCAGNAAPMPAIRVAIDAGGRFVVSNDAPADSDA
RDRSDASDASDASDAIDAVDAVDAVDAPNAPNAPNAPNAPNAPNAPNAPNAR*

  Nucleotide sequence: 3039 bp    

>T6CP000668 NC_006351:2046791-2049829 [Burkholderia pseudomallei K96243] [TssH]
ATGCTGCTGGTTGACCTGAAACCGTTGATCGAACAGCTCAACCCCTATTGCCGGAACGCGCTCGAAAGCGCCGTCGGCGC
GTGCGTCGCGCGCCGGCACGACGACGTCGCCGTCGAGCACCTGCTCGCGCGGCTGTGCGACGAACCGTCGGCCGACGTCG
CGCTGTTGCTGCGCGCGTGCGGCGCGGACGCGGCGCGCCTGCGCCGGCAGGCCGACGCCGCGCTCGACGCGCGCCCGGCG
GGCGACGGCGGCCGCCCCGCGTTCGCGCCGTCGCTGCTCGCGCTGTTGCAGGACGCGTGGCTGATCGCGTCGCTCGAGCT
CGGCGACACGCACATCCGCTCGGCGGCCGTGCTCGCCGCCGCGGTCGCCCGCGCGGCGCGGCAGCCGTCGCCCGGCGGCG
ACGACGTGCTGCAGTCGCTGCGCAAGGACGCGCTCGTCGCGCGCTTTGCGAGCGGCGCGTGCGCGCGATCGATCGAATCG
CGCGCGTCCGGCGCGCTCGACGCCGCGCCGGGCGAGGCGAGCGACACGCGCGACGCATCGAGCGCGATCGCCCGCTACTG
CGAGGACTTCACCGCGAAAGCGCGCGCCGGCGGGATCGATCCGGTCTTCGGTCGCGACGCGGAAATCCGCCAGATCGTCG
ACATCCTCGCGCGGCGGCGCAAGAACAATCCGGTGTGCGTCGGCGAGCCGGGCGTGGGCAAGACGGCCGTCGTCGAAGGG
CTCGCGCTGCGGATCGCCGAAGGCGACGTGCCGGCGACGCTGCGCGGCGCGACGCTGCTCGGCCTCGACCTCGGCATGCT
GCAGGCGGGCGCGAGCGTGAAGGGCGAATTCGAGCAGCGGCTCAAGCGCGTGATCGCCGAGATCCGCGCGTCGCAAACGC
CCGTCGTGCTGTTCATCGACGAAGCGCACACGCTGATCGGCGCGGGCGGCGCGGCGGGCGCGTCCGACGCGGCGAACCTG
CTGAAGCCCGCGCTCGCGCGCGGCGAGCTGCGCACGATCGCGGCCACCACGTGGAGCGAATACAAGAAGTATTTCGAGAA
GGACGCGGCGCTCGCGCGGCGCTTCCAGCCGGTGAAGCTCGACAGCCCGAACGTCGCGACGTCGGTGATGATCCTGCGCG
GGCTGAAGGAGCGCTATCAGGACGCGCACGGCGTGACGATCCGCGACGACGCGCTCGTCGCCGCGGCCGAGCTGTCGGCG
CGCTACATCACCGGTCGGCAGTTGCCGGACAAGGCGATCGACCTGCTCGACACCGCGTGCGCGCGCGTGAAGGTGCGCCA
GCAGACCAAGCCCGCCGCGCTGGAGGACGCGCAGCGCGCGATCCAGGCGCTCGAGCGCGAGCGGCGCGCGCTGCGCGACG
AATTGGCCGAGCGCTGCGCGCCGGATACGCCGCGCGTCGCCGACATCGATCGCGAGCTCGCCGCGCTGTCCGCGCGCGCC
GGCGCGCTGCGCGACGCGTGGGCGGCGCAGCGCGACGCCGCGCAGGCGCTCGTCGACGCACGCCGCGCGTGCCGGGCGGC
GGCGGATGCAACGGATGCGGCTGGAACGACCGGAACGGCCCATGCCGGCGAGCTCGCCGATAGTGCCGACGTTTCCGATA
TCGCCGATGTCGCCAAGATGGCCGATGCGGCCGACGCCGCCCCTGCCGATGCCGAAACCGGCTCGAACGCCCGCGACGCC
GACGTACGCATGCCCGCCGGGACGTGCGCGCGCATCGCCCCGAACCCGGATGCGCGGGCGCGGCCCCCTTTGCGCGCGCC
GCACGCCGACGCGCGCGAGCGCGCGGCTCGTGCGCTCGCCGAAGCGACGCGCCGCTTCGAGCACGCGCAGCGCGACACGC
CGCTCGTGCGAATCGACGTCGATCCGGACGCGATCGCCGACGTCGTGGCCGACTGGACCGGCATCGCGGCCGGCAAGCTG
CGCCGCGATCGCGCGAACGTGATGCTGCGGCTCGCCGATACGCTGCGCCGCCGCATCCGCGGCCAGGATCACGCGATCGA
ACAGATCTCCGAAGCCGTGAAGGCGGGCGCGGCCGGCGTCCACGATCCGCGCCGCCCGCTCGGCGTGTTCCTGCTCGCGG
GGCCGTCGGGCACCGGCAAGACCGAGACCGCGCTCGCGGTCGCCGATGCGCTGTTCGGCGACGAGCGCTCGATCGTCGTC
GTCAACATGAGCGAATTCCAGGAGCGGCACGACGTGAGCCGCCTGATCGGCTCGCCGCCGGGCTACGTCGGCTACGGCGA
GGGCGGGATGCTGACCGAGGCGGTGCGCCAGCGGCCCTATTCGGTCGTGCTGCTAGACGAAGTCGAGAAGGCGCACCCCG
ACGTGCTGAACCTGTTCTACCAGGTGTTCGACAAGGGTTCGCTGTCCGACGGCGAAGGCAAGGAGGTCGACTTCGCGAAC
ACGGTGATCTTCCTCACGTCGAATCTCGGCGCGGACATCATCGCCGACATCGCCGCACGCGGCGCGCGGCCCGATCCGGA
CGCGATGCGCGCGGCGGTGCGCCCGGCGCTGTCGCGCCATTTCAAGCCGGCGCTGCTCGCGCGGATGACCGAGATTCCGT
ATGCGCCGCTCGCGCCCGATACGCTCGCCGACATCGCGCGGCTGAAGCTCGAGCGCATCGCGGCGCGCGTCGCCGCGCAG
CACGCGACGCGCATCGTCTACGACGACGCCGTCGTCGCGCACGTCGCCGCGCGCTGCACCGAAGTCGAATCGGGCGCGCG
CAACGTCGATTTCATCCTGCAGCGCCACGTGCTGCCCGCGCTCGCGCAGCACGTGCTCGTGTGCGCGGGCAACGCGGCGC
CGATGCCTGCGATTCGCGTCGCCATCGACGCCGGCGGCCGCTTCGTCGTCTCGAACGACGCGCCGGCCGACTCCGATGCA
CGGGACCGGAGCGATGCGTCCGATGCGTCCGATGCGTCTGATGCGATCGACGCGGTCGACGCGGTCGATGCGGTCGATGC
ACCCAACGCACCCAACGCACCCAACGCACCCAACGCACCCAACGCACCCAACGCACCCAACGCACCCAACGCACGCTGA