Detailed information of component protein


Summary

Component ID T6CP012442
Component protein type VirA
T6SS ID (Type) T6SS00075 experimental (Type i1)
Strain Burkholderia pseudomallei K96243
Replicon chromosome II
Sequence Protein sequence (615 a.a.); Nucleotide sequence (1845 bp)
Reference
[1] Burtnick MN, Brett PJ, Harding SV, et al. The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomallei. Infect Immun. 2011 Apr;79(4):1512-25. doi: 10.1128/IAI.01218-10. Epub 2011 Feb 7. PMID: 21300775
[2] Shalom G, Shaw JG, Thomas MS. In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages. Microbiology (Reading). 2007 Aug;153(Pt 8):2689-2699. doi: 10.1099/mic.0.2007/006585-0. PMID: 17660433
[3] Chen Y, Wong J, Sun GW, et al. Regulation of type VI secretion system during Burkholderia pseudomallei infection. Infect Immun. 2011 Aug;79(8):3064-73. doi: 10.1128/IAI.05148-11. Epub 2011 Jun 13. PMID: 21670170
[4] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
[5] Sun GW, Chen Y, Liu Y, et al. Identification of a regulatory cascade controlling Type III Secretion System 3 gene expression in Burkholderia pseudomallei. Mol Microbiol. 2010 May;76(3):677-89. doi: 10.1111/j.1365-2958.2010.07124.x. Epub 2010 Mar 24. PMID: 20345664
[6] Toesca IJ, French CT, Miller JF. The Type VI secretion system spike protein VgrG5 mediates membrane fusion during intercellular spread by pseudomallei group Burkholderia species. Infect Immun. 2014 Apr;82(4):1436-44. doi: 10.1128/IAI.01367-13. Epub 2014 Jan 13. PMID: 24421040
[7] Hopf V, Göhler A, Eske-Pogodda K, et al. BPSS1504, a cluster 1 type VI secretion gene, is involved in intracellular survival and virulence of Burkholderia pseudomallei. Infect Immun. 2014 May;82(5):2006-15. doi: 10.1128/IAI.01544-14. Epub 2014 Mar 4. PMID: 24595140
[8] Chen Y, Schröder I, French CT, et al. Characterization and analysis of the Burkholderia pseudomallei BsaN virulence regulon. BMC Microbiol. 2014 Aug 1;14:206. doi: 10.1186/s12866-014-0206-6. PMID: 25085508
[9] Chirakul S, Bartpho T, Wongsurawat T, et al. Characterization of BPSS1521 (bprD), a regulator of Burkholderia pseudomallei virulence gene expression in the mouse model. PLoS One. 2014 Aug 11;9(8):e104313. doi: 10.1371/journal.pone.0104313. eCollection 2014. PMID: 25111708
[10] Chen Y, Schröder I, French CT, et al. Characterization and analysis of the Burkholderia pseudomallei BsaN virulence regulon. BMC Microbiol. 2014 Aug 1;14:206. doi: 10.1186/s12866-014-0206-6. PMID: 25085508
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BPS_RS26880
Coordinate (Strand) 2038711..2040555 (-)
NCBI ID WP_011205628.1
RefSeq NC_006351
Uniprot ID Q63K70_BURPS
KEGG ID bps:BPSS1495
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
HATPase_c PF02518.28 3.1e-18 454..592
MASE1 PF05231.16 5.2e-14 63..323
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-32 MHGNSIDAGPPARARPARITRTARAAPRRADR
Transmembrane helix 33-52 AFAPGGALNAAGLLLLLFYA
Outside 53-66 NHLLSRPGACTSLN
Transmembrane helix 67-86 HTLSAVVWPAPVLGIALLLH
Inside 87-92 CRRPLA
Transmembrane helix 93-115 WWLGAGSLFATLLAAGSLDWVPW
Outside 116-118 HVD
Transmembrane helix 119-141 ALFAAVNVVEAIAGAWLARRYLI
Inside 142-153 AGGGVDTLRGFA
Transmembrane helix 154-176 MFVLLLPLGMAAMHATLAAAILS
Outside 177-190 VWMNDREWLSEWSR
Transmembrane helix 191-213 AYAANALALLVLLPALLTWNARA
Inside 214-224 CGATWSRSRNQ
Transmembrane helix 225-247 LPVLATFGSLCFASIWGYQAEVA
Outside 248-261 RALLALSLSWAALE
Transmembrane helix 262-284 GGLVLASQLNALSAIALIAMTLG
Inside 285-296 GRGPYAWHHDGH
Transmembrane helix 297-319 GVWALQVDLIGVAILSMCIAIAT
Outside 320-614 GERRRLARQIERSRRFESLGFFAGGIVHDFSNVLTTIDCHAEYASERLASGARADDSIDEIHRAVERGRDLAAQILLAARRGEPVLTRVSVDEVVDEALAATRAAAWRGVEIVRCGDGARDGARRGDVRHVVFADRSQLARAVMNLVGNAVRAARATVVVRVGVNGGVNGGASDGASRGANDGGAPAARAFDVAIGEASMPGGVWIEVSDDGEGIDVEPLDRIFEPFYSNRSGAPGTGLGLAIVAGAALAHRGQIAVATDRGVGSCFRFTLPAAWGTGAPGAMDAGAPGAVQERT


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.8347 - -


  Protein sequence: 615 a.a.    

>T6CP012442 NC_006351:c2040555-2038711 [Burkholderia pseudomallei K96243] [VirA]
MHGNSIDAGPPARARPARITRTARAAPRRADRAFAPGGALNAAGLLLLLFYANHLLSRPGACTSLNHTLSAVVWPAPVLG
IALLLHCRRPLAWWLGAGSLFATLLAAGSLDWVPWHVDALFAAVNVVEAIAGAWLARRYLIAGGGVDTLRGFAMFVLLLP
LGMAAMHATLAAAILSVWMNDREWLSEWSRAYAANALALLVLLPALLTWNARACGATWSRSRNQLPVLATFGSLCFASIW
GYQAEVARALLALSLSWAALEGGLVLASQLNALSAIALIAMTLGGRGPYAWHHDGHGVWALQVDLIGVAILSMCIAIATG
ERRRLARQIERSRRFESLGFFAGGIVHDFSNVLTTIDCHAEYASERLASGARADDSIDEIHRAVERGRDLAAQILLAARR
GEPVLTRVSVDEVVDEALAATRAAAWRGVEIVRCGDGARDGARRGDVRHVVFADRSQLARAVMNLVGNAVRAARATVVVR
VGVNGGVNGGASDGASRGANDGGAPAARAFDVAIGEASMPGGVWIEVSDDGEGIDVEPLDRIFEPFYSNRSGAPGTGLGL
AIVAGAALAHRGQIAVATDRGVGSCFRFTLPAAWGTGAPGAMDAGAPGAVQERT*

  Nucleotide sequence: 1845 bp    

>T6CP012442 NC_006351:c2040555-2038711 [Burkholderia pseudomallei K96243] [VirA]
ATGCACGGAAATTCCATCGATGCCGGGCCGCCCGCGCGCGCCCGGCCCGCACGCATCACGCGAACGGCGCGCGCCGCGCC
GCGCCGCGCCGATCGCGCGTTCGCGCCGGGCGGCGCGCTGAACGCGGCGGGGCTGCTGCTGCTGCTGTTCTACGCGAATC
ATCTGCTGTCCCGGCCCGGCGCGTGCACGTCGCTCAATCACACGCTGTCGGCCGTCGTGTGGCCCGCGCCGGTGCTCGGC
ATCGCGCTGCTGCTGCACTGCCGGCGGCCGCTCGCATGGTGGCTCGGCGCGGGTTCGCTGTTCGCCACGCTGCTCGCCGC
GGGCTCGCTCGACTGGGTGCCGTGGCACGTCGACGCGCTGTTCGCGGCCGTCAACGTCGTCGAGGCGATCGCGGGCGCGT
GGCTCGCGCGGCGCTATCTGATCGCGGGCGGCGGGGTGGACACGCTGCGCGGCTTCGCGATGTTCGTGCTGCTGCTGCCG
CTCGGCATGGCGGCGATGCACGCGACGCTCGCGGCGGCGATCCTGTCGGTGTGGATGAACGACCGCGAATGGCTGAGCGA
ATGGTCGCGCGCGTATGCGGCGAACGCGCTCGCGCTGCTCGTGCTGCTGCCGGCGCTCCTCACGTGGAACGCGCGCGCGT
GCGGCGCGACGTGGTCGCGCAGCCGCAATCAGTTGCCGGTGCTCGCGACGTTCGGCTCGCTGTGCTTCGCGTCGATATGG
GGATACCAGGCCGAGGTCGCGCGCGCGCTGCTCGCGCTGTCGCTGAGCTGGGCCGCGCTCGAGGGCGGCCTCGTGCTCGC
GTCCCAGCTCAACGCGCTCTCGGCGATCGCCCTGATCGCGATGACGCTCGGCGGGCGCGGCCCGTATGCGTGGCATCACG
ACGGCCACGGCGTCTGGGCGCTGCAGGTCGATCTGATCGGCGTCGCGATCCTGTCGATGTGCATCGCGATCGCGACGGGC
GAGCGGCGGCGGCTCGCGCGGCAGATCGAACGATCGCGCCGTTTCGAGTCGCTCGGCTTTTTCGCCGGCGGAATCGTGCA
CGATTTCAGCAACGTGCTGACGACGATCGATTGCCACGCCGAATACGCGTCCGAGCGGCTCGCGAGCGGCGCGCGCGCGG
ACGATTCGATCGACGAGATTCATCGCGCGGTCGAACGCGGCCGCGATCTGGCGGCGCAGATCCTGCTCGCCGCGCGCCGC
GGCGAGCCCGTGCTGACGCGGGTGAGCGTCGACGAGGTTGTCGACGAGGCGCTCGCCGCGACACGCGCGGCCGCGTGGCG
CGGCGTCGAGATCGTTCGATGCGGCGATGGCGCGCGCGACGGCGCCCGGCGTGGCGACGTTCGTCACGTCGTATTCGCCG
ACCGCAGCCAGCTCGCGCGCGCGGTCATGAATCTCGTCGGCAATGCGGTGCGCGCGGCGCGCGCGACGGTTGTCGTGCGT
GTCGGGGTGAACGGCGGGGTGAACGGCGGCGCGAGCGATGGGGCGAGCCGCGGCGCGAACGACGGCGGCGCGCCGGCCGC
TCGCGCATTCGACGTCGCGATCGGCGAAGCGTCGATGCCGGGCGGCGTGTGGATCGAGGTGAGCGACGACGGCGAAGGCA
TCGACGTCGAGCCGCTCGACCGCATCTTCGAGCCGTTCTATTCGAACCGCTCCGGCGCGCCGGGGACGGGGCTGGGTCTC
GCGATCGTCGCGGGCGCGGCGCTCGCGCACCGCGGGCAGATCGCCGTCGCGACGGATCGGGGCGTGGGCAGTTGCTTTCG
GTTCACGTTGCCGGCGGCATGGGGAACGGGCGCGCCCGGCGCAATGGACGCCGGCGCGCCCGGCGCAGTACAGGAGAGGA
CATGA