Detailed information of component protein
Summary
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | BPS_RS26945 |
| Coordinate (Strand) | 2057809..2058606 (+) |
| NCBI ID | WP_004533362.1 |
| RefSeq | NC_006351 |
| Uniprot ID | B1H8J4_BURPE |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 9.8e-19 | 72..180 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-265 | MNARHIAPPAATRARRPMQPGRPSWPSRTYRACAARTCAAVLVALAALAGCAARVTEEQARADVRWDYAPDALRIDIDASARLNEYLNAPHTLLLAVFQSADAKAFRQLADDPDRLRMTLAAGGPAADFIQTTRYVVAPGQHVALSIDRAQQARYVGIVAGYYDADGPRAARLFEVPLRIDKRGWFSSTYRAAPRTLELKLRLGAQSITDAREAPLNLPPPGARAWTALDSGAKTLALPSADANEGGSGKRDKRGNAAARAPRKQ |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.7656 | pos: 50-51. LAG-CA. | MNARHIAPPAATRARRPMQPGRPSWPSRTYRACAARTCAAVLVALAALAG |
Protein sequence: 266 a.a.
>T6CP009348 NC_006351:2057809-2058606 [Burkholderia pseudomallei K96243] [TssJ]
MNARHIAPPAATRARRPMQPGRPSWPSRTYRACAARTCAAVLVALAALAGCAARVTEEQARADVRWDYAPDALRIDIDAS
ARLNEYLNAPHTLLLAVFQSADAKAFRQLADDPDRLRMTLAAGGPAADFIQTTRYVVAPGQHVALSIDRAQQARYVGIVA
GYYDADGPRAARLFEVPLRIDKRGWFSSTYRAAPRTLELKLRLGAQSITDAREAPLNLPPPGARAWTALDSGAKTLALPS
ADANEGGSGKRDKRGNAAARAPRKQ*
MNARHIAPPAATRARRPMQPGRPSWPSRTYRACAARTCAAVLVALAALAGCAARVTEEQARADVRWDYAPDALRIDIDAS
ARLNEYLNAPHTLLLAVFQSADAKAFRQLADDPDRLRMTLAAGGPAADFIQTTRYVVAPGQHVALSIDRAQQARYVGIVA
GYYDADGPRAARLFEVPLRIDKRGWFSSTYRAAPRTLELKLRLGAQSITDAREAPLNLPPPGARAWTALDSGAKTLALPS
ADANEGGSGKRDKRGNAAARAPRKQ*
Nucleotide sequence: 798 bp
>T6CP009348 NC_006351:2057809-2058606 [Burkholderia pseudomallei K96243] [TssJ]
ATGAACGCACGACACATTGCCCCGCCGGCCGCGACGCGCGCGCGCCGGCCGATGCAGCCCGGCCGGCCGTCATGGCCGTC
GCGAACGTATCGCGCATGCGCGGCTCGCACATGCGCTGCCGTGCTCGTCGCGCTCGCCGCCCTCGCCGGCTGTGCGGCCC
GCGTCACCGAAGAGCAGGCGCGCGCCGACGTGCGATGGGACTACGCGCCCGACGCGCTGCGCATCGATATCGACGCGTCG
GCGCGGTTGAACGAATACCTGAACGCGCCGCACACGCTGTTGCTCGCGGTGTTCCAGAGCGCGGACGCGAAGGCGTTCCG
CCAGCTCGCCGACGATCCGGACCGGCTGCGCATGACGCTCGCGGCGGGCGGCCCCGCCGCGGACTTCATTCAGACGACCC
GCTACGTCGTCGCGCCCGGCCAGCATGTCGCGCTGTCGATCGATCGCGCGCAGCAGGCGCGCTACGTCGGCATCGTCGCC
GGCTACTACGACGCCGACGGCCCACGCGCGGCGCGCCTGTTCGAGGTGCCGCTGCGCATCGACAAGCGCGGCTGGTTCTC
CTCCACCTATCGCGCCGCGCCGCGAACGCTCGAGCTGAAGCTCAGGCTCGGCGCGCAGAGCATCACCGACGCGCGCGAAG
CGCCGCTGAACCTGCCGCCGCCGGGCGCGCGCGCATGGACGGCGCTCGACAGCGGCGCGAAGACCCTGGCGCTGCCGAGC
GCCGACGCGAACGAGGGCGGCAGCGGCAAGCGCGACAAACGCGGCAACGCCGCCGCCCGTGCGCCGCGCAAGCAATGA
ATGAACGCACGACACATTGCCCCGCCGGCCGCGACGCGCGCGCGCCGGCCGATGCAGCCCGGCCGGCCGTCATGGCCGTC
GCGAACGTATCGCGCATGCGCGGCTCGCACATGCGCTGCCGTGCTCGTCGCGCTCGCCGCCCTCGCCGGCTGTGCGGCCC
GCGTCACCGAAGAGCAGGCGCGCGCCGACGTGCGATGGGACTACGCGCCCGACGCGCTGCGCATCGATATCGACGCGTCG
GCGCGGTTGAACGAATACCTGAACGCGCCGCACACGCTGTTGCTCGCGGTGTTCCAGAGCGCGGACGCGAAGGCGTTCCG
CCAGCTCGCCGACGATCCGGACCGGCTGCGCATGACGCTCGCGGCGGGCGGCCCCGCCGCGGACTTCATTCAGACGACCC
GCTACGTCGTCGCGCCCGGCCAGCATGTCGCGCTGTCGATCGATCGCGCGCAGCAGGCGCGCTACGTCGGCATCGTCGCC
GGCTACTACGACGCCGACGGCCCACGCGCGGCGCGCCTGTTCGAGGTGCCGCTGCGCATCGACAAGCGCGGCTGGTTCTC
CTCCACCTATCGCGCCGCGCCGCGAACGCTCGAGCTGAAGCTCAGGCTCGGCGCGCAGAGCATCACCGACGCGCGCGAAG
CGCCGCTGAACCTGCCGCCGCCGGGCGCGCGCGCATGGACGGCGCTCGACAGCGGCGCGAAGACCCTGGCGCTGCCGAGC
GCCGACGCGAACGAGGGCGGCAGCGGCAAGCGCGACAAACGCGGCAACGCCGCCGCCCGTGCGCCGCGCAAGCAATGA