Detailed information of component protein


Summary

Component ID T6CP000672
Component protein type TssM
T6SS ID (Type) T6SS00075 experimental (Type i1)
Strain Burkholderia pseudomallei K96243
Replicon chromosome II
Sequence Protein sequence (1325 a.a.); Nucleotide sequence (3975 bp)
Reference
[1] Burtnick MN, Brett PJ, Harding SV, et al. The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomallei. Infect Immun. 2011 Apr;79(4):1512-25. doi: 10.1128/IAI.01218-10. Epub 2011 Feb 7. PMID: 21300775
[2] Shalom G, Shaw JG, Thomas MS. In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages. Microbiology (Reading). 2007 Aug;153(Pt 8):2689-2699. doi: 10.1099/mic.0.2007/006585-0. PMID: 17660433
[3] Chen Y, Wong J, Sun GW, et al. Regulation of type VI secretion system during Burkholderia pseudomallei infection. Infect Immun. 2011 Aug;79(8):3064-73. doi: 10.1128/IAI.05148-11. Epub 2011 Jun 13. PMID: 21670170
[4] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
[5] Sun GW, Chen Y, Liu Y, et al. Identification of a regulatory cascade controlling Type III Secretion System 3 gene expression in Burkholderia pseudomallei. Mol Microbiol. 2010 May;76(3):677-89. doi: 10.1111/j.1365-2958.2010.07124.x. Epub 2010 Mar 24. PMID: 20345664
[6] Toesca IJ, French CT, Miller JF. The Type VI secretion system spike protein VgrG5 mediates membrane fusion during intercellular spread by pseudomallei group Burkholderia species. Infect Immun. 2014 Apr;82(4):1436-44. doi: 10.1128/IAI.01367-13. Epub 2014 Jan 13. PMID: 24421040
[7] Hopf V, Göhler A, Eske-Pogodda K, et al. BPSS1504, a cluster 1 type VI secretion gene, is involved in intracellular survival and virulence of Burkholderia pseudomallei. Infect Immun. 2014 May;82(5):2006-15. doi: 10.1128/IAI.01544-14. Epub 2014 Mar 4. PMID: 24595140
[8] Chen Y, Schröder I, French CT, et al. Characterization and analysis of the Burkholderia pseudomallei BsaN virulence regulon. BMC Microbiol. 2014 Aug 1;14:206. doi: 10.1186/s12866-014-0206-6. PMID: 25085508
[9] Chirakul S, Bartpho T, Wongsurawat T, et al. Characterization of BPSS1521 (bprD), a regulator of Burkholderia pseudomallei virulence gene expression in the mouse model. PLoS One. 2014 Aug 11;9(8):e104313. doi: 10.1371/journal.pone.0104313. eCollection 2014. PMID: 25111708
[10] Chen Y, Schröder I, French CT, et al. Characterization and analysis of the Burkholderia pseudomallei BsaN virulence regulon. BMC Microbiol. 2014 Aug 1;14:206. doi: 10.1186/s12866-014-0206-6. PMID: 25085508
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BPS_RS26960
Coordinate (Strand) 2060695..2064669 (+)
NCBI ID WP_181937968.1
RefSeq NC_006351
Uniprot ID UPI000A9EBFEE
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 9.3e-56 177..420
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-6 MKTLGM
Transmembrane helix 7-26 MIARIAALLALAALSWASAL
Outside 27-29 YFD
Transmembrane helix 30-52 WPLWCAPAVFCAALAGWLLCRVA
Inside 53-414 RRALRAVRARAQLARLDASERLPAADAPELRVAARWRTALAALGRAQPAPRLRGRPRDALPWYLVIGPEGAGKTTALARARLVSPLRHARDDAAIAPTEDCDWWCFDDAVVLDLAGRFAAPGATDDDRRAWHALLEQLGRARARRGVNGVVVAIDAPRLIEASRDALTIEGSAIRERLEQLIRLFDRRFPVYVLVTQCDRLYGFDEWAAQLAPEQRERAFGYLGDHDADAFVARALDSLDARLGALRVALAARGEPPSPRALMLPHELACLRPALEAFARAAFGPNVYQETPYLRGLLFASGRQAGGAPSLTLPDWLDAAPARTPGDAGLFLHDLFARVLPGERDAWLPVERPSRSRLVLRR
Transmembrane helix 415-437 LALAAWLLASIAAGLLMSASFVG
Outside 438-1324 DMRTVELVRRDYPAHPRFTGEFAHDTATLERIGRVIADVERRDERRLVRPLAGATPVGRLEAELKRRYVAHYRRSIEPTADRLFFGEPDGASDVRAADDARLAARIRNLVRYVNLMQARRRGADRETLAQMPAPAVARATDGGGARGVDSVGVSGVSGVSGVSGGVGGVGGARAAEGAGRAVRASGGRRAGDARAARGMRGAAGNAGAGAEIAPAADGVRDRDEGFARVSALAVDLIAWSAPDDRALAARVAAAQAELERLAYRDPDGAWLLALPDASMPRDVTLADFWPAAEPRTLPDAQPAALRDVRVPAALTAAHRPAIDAFLDEMAQAVANRPKFAFHRDAFDTWYRARRIDAWRDFVARFPQGEQGLTTQAQWRAVIDRIADRRDPFSALLARVDREFESERDDTLPPWLRFVRTTSHMLAPAMLAPARLPAASGGLGAALGSISRSGGRALREALGGAPEQGRLTLERDAALRDALVDYERRVAALAADALAGPGAAYRLAADFHGFGVDPSVQASAMRAADSALRDVKRLAGDRDVGGDVVWSLVGGPLRALIAYVERQASCALQDDWERDVLWPLRRAATRDDVEGLLYGPQGAIWAFVDGPAKPFVRVGAARASALDTLGYRLPFTDAFLPLIDDAAARRVAQARRDAERRAQQQAALELDERIASLGKQIDALRAQTVRFGIVAQPTDVNPDAQAKPFETVLTLQCAPQARTLTNYNLRVSEQIDWQPDRCGDATLRISLGGVTLVRRYAGSLGVARFVQDFRYGVRRFTPRDFPDAKAQLQRLGVRHIDVRYDFSGHDALLAHVGRIDALERARRDDLARQRRAANRQDDDAGGATAIARAAASVANAPGAALPRRIGVCWGDPAHDRPDGDGAQP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.8003 - -


  Protein sequence: 1325 a.a.    

>T6CP000672 NC_006351:2060695-2064669 [Burkholderia pseudomallei K96243] [TssM]
MKTLGMMIARIAALLALAALSWASALYFDWPLWCAPAVFCAALAGWLLCRVARRALRAVRARAQLARLDASERLPAADAP
ELRVAARWRTALAALGRAQPAPRLRGRPRDALPWYLVIGPEGAGKTTALARARLVSPLRHARDDAAIAPTEDCDWWCFDD
AVVLDLAGRFAAPGATDDDRRAWHALLEQLGRARARRGVNGVVVAIDAPRLIEASRDALTIEGSAIRERLEQLIRLFDRR
FPVYVLVTQCDRLYGFDEWAAQLAPEQRERAFGYLGDHDADAFVARALDSLDARLGALRVALAARGEPPSPRALMLPHEL
ACLRPALEAFARAAFGPNVYQETPYLRGLLFASGRQAGGAPSLTLPDWLDAAPARTPGDAGLFLHDLFARVLPGERDAWL
PVERPSRSRLVLRRLALAAWLLASIAAGLLMSASFVGDMRTVELVRRDYPAHPRFTGEFAHDTATLERIGRVIADVERRD
ERRLVRPLAGATPVGRLEAELKRRYVAHYRRSIEPTADRLFFGEPDGASDVRAADDARLAARIRNLVRYVNLMQARRRGA
DRETLAQMPAPAVARATDGGGARGVDSVGVSGVSGVSGVSGGVGGVGGARAAEGAGRAVRASGGRRAGDARAARGMRGAA
GNAGAGAEIAPAADGVRDRDEGFARVSALAVDLIAWSAPDDRALAARVAAAQAELERLAYRDPDGAWLLALPDASMPRDV
TLADFWPAAEPRTLPDAQPAALRDVRVPAALTAAHRPAIDAFLDEMAQAVANRPKFAFHRDAFDTWYRARRIDAWRDFVA
RFPQGEQGLTTQAQWRAVIDRIADRRDPFSALLARVDREFESERDDTLPPWLRFVRTTSHMLAPAMLAPARLPAASGGLG
AALGSISRSGGRALREALGGAPEQGRLTLERDAALRDALVDYERRVAALAADALAGPGAAYRLAADFHGFGVDPSVQASA
MRAADSALRDVKRLAGDRDVGGDVVWSLVGGPLRALIAYVERQASCALQDDWERDVLWPLRRAATRDDVEGLLYGPQGAI
WAFVDGPAKPFVRVGAARASALDTLGYRLPFTDAFLPLIDDAAARRVAQARRDAERRAQQQAALELDERIASLGKQIDAL
RAQTVRFGIVAQPTDVNPDAQAKPFETVLTLQCAPQARTLTNYNLRVSEQIDWQPDRCGDATLRISLGGVTLVRRYAGSL
GVARFVQDFRYGVRRFTPRDFPDAKAQLQRLGVRHIDVRYDFSGHDALLAHVGRIDALERARRDDLARQRRAANRQDDDA
GGATAIARAAASVANAPGAALPRRIGVCWGDPAHDRPDGDGAQP*

  Nucleotide sequence: 3975 bp    

>T6CP000672 NC_006351:2060695-2064669 [Burkholderia pseudomallei K96243] [TssM]
ATGAAGACTCTCGGCATGATGATCGCGCGGATCGCCGCGCTGCTCGCGCTCGCCGCGCTCAGTTGGGCGAGCGCGCTCTA
TTTCGACTGGCCGCTGTGGTGCGCGCCCGCCGTGTTCTGCGCGGCGCTCGCCGGATGGCTGCTGTGCCGCGTCGCGCGCC
GCGCGCTGCGCGCGGTTCGTGCGCGCGCGCAGCTCGCACGGCTCGACGCATCCGAGCGCCTGCCCGCGGCCGACGCGCCG
GAGCTGCGCGTCGCCGCGCGCTGGCGCACCGCGCTCGCCGCGCTCGGGCGCGCGCAGCCCGCGCCGCGCCTGCGCGGCCG
GCCGCGCGACGCGCTGCCGTGGTATCTCGTGATCGGCCCCGAGGGCGCGGGCAAGACAACCGCGCTCGCACGCGCGCGCC
TCGTGTCGCCGCTGCGGCACGCGCGCGACGACGCGGCCATCGCGCCGACCGAGGACTGCGACTGGTGGTGCTTCGACGAC
GCGGTCGTGCTCGATCTCGCGGGCCGCTTCGCCGCGCCCGGCGCGACCGACGACGATCGCCGCGCATGGCACGCGCTCCT
CGAGCAGCTCGGCCGCGCGCGTGCGCGCCGGGGCGTGAACGGCGTCGTGGTCGCGATCGACGCGCCGCGCCTCATCGAAG
CGAGCCGCGATGCGCTGACGATCGAAGGCAGCGCCATTCGCGAACGGCTCGAGCAACTGATCCGCCTGTTCGACCGGCGC
TTTCCGGTGTACGTGCTCGTCACGCAATGCGACCGGCTCTACGGCTTCGACGAATGGGCCGCGCAGCTCGCGCCGGAACA
ACGCGAGCGCGCGTTCGGCTATCTCGGCGATCACGACGCGGATGCGTTCGTCGCGCGCGCGCTCGACAGCCTCGACGCAC
GGCTCGGCGCGCTGCGCGTCGCGCTGGCGGCGCGCGGCGAGCCGCCGTCGCCGCGCGCGCTGATGCTGCCGCACGAACTC
GCGTGCCTGCGGCCCGCGCTCGAGGCGTTCGCACGCGCGGCGTTCGGCCCGAACGTCTATCAGGAAACACCGTATCTGCG
CGGGCTGCTGTTCGCGAGCGGCCGGCAGGCGGGCGGCGCGCCGTCGCTGACGCTGCCCGACTGGCTCGACGCCGCGCCGG
CGCGCACGCCCGGCGATGCGGGCCTGTTCCTGCACGACTTGTTCGCGCGCGTGCTGCCGGGCGAGCGCGACGCGTGGCTG
CCCGTCGAGCGGCCGAGCCGCTCGCGGCTCGTGCTGCGGCGCCTCGCGCTCGCCGCGTGGCTCCTCGCGAGCATCGCGGC
CGGGCTGCTGATGAGCGCATCGTTCGTCGGCGACATGCGAACCGTCGAGCTGGTCCGCCGCGACTATCCCGCGCATCCGC
GCTTCACCGGCGAATTCGCGCACGACACGGCGACGCTCGAGCGGATCGGCCGCGTGATCGCCGACGTCGAGCGGCGCGAC
GAGCGGCGGCTCGTGCGCCCGCTCGCCGGCGCGACGCCCGTCGGCCGGCTCGAAGCGGAACTCAAGCGCCGCTACGTCGC
GCACTATCGGCGCTCGATCGAACCCACCGCCGATCGGCTGTTCTTCGGCGAGCCGGACGGCGCGAGCGACGTGCGCGCGG
CCGACGATGCGCGGCTCGCCGCGCGCATCCGCAATCTGGTCCGCTACGTGAACCTGATGCAGGCGCGCCGGCGCGGCGCG
GATCGCGAGACGCTCGCGCAAATGCCCGCACCGGCGGTCGCGCGCGCGACGGATGGCGGTGGCGCTCGCGGAGTGGACAG
TGTGGGTGTTTCTGGTGTTTCTGGTGTTTCTGGTGTTTCTGGCGGTGTAGGCGGTGTAGGCGGTGCGCGCGCCGCGGAAG
GTGCGGGTCGCGCCGTCCGCGCGAGCGGCGGACGGCGCGCAGGCGACGCGCGCGCCGCGCGCGGCATGCGCGGCGCCGCC
GGCAACGCCGGCGCCGGCGCTGAAATCGCACCCGCCGCAGACGGCGTTCGCGATCGCGACGAAGGGTTTGCGCGCGTCAG
CGCGCTCGCCGTCGACCTGATCGCGTGGAGCGCGCCCGACGATCGCGCGCTCGCCGCGCGCGTCGCGGCCGCGCAGGCAG
AGCTCGAACGGCTCGCGTACCGCGATCCGGACGGCGCGTGGCTGCTCGCGCTACCGGATGCCTCGATGCCGCGCGACGTG
ACGCTCGCCGATTTCTGGCCCGCCGCCGAGCCGCGCACGCTGCCCGACGCACAGCCCGCCGCGCTGCGCGACGTGCGCGT
GCCCGCCGCGCTGACGGCCGCGCATCGCCCGGCCATCGATGCGTTCCTCGACGAGATGGCGCAGGCGGTCGCGAACCGGC
CGAAGTTCGCGTTCCATCGCGACGCGTTCGACACGTGGTACCGCGCACGGCGCATCGACGCATGGCGCGACTTCGTCGCA
CGCTTCCCGCAGGGCGAGCAAGGGCTGACGACGCAAGCGCAATGGCGCGCGGTGATCGACCGGATCGCCGATCGCCGCGA
TCCGTTCTCGGCATTGCTCGCGCGCGTCGATCGCGAATTCGAATCCGAGCGCGACGACACGCTGCCGCCGTGGCTACGGT
TCGTGCGCACGACATCGCACATGCTCGCCCCCGCGATGCTCGCGCCCGCGCGCCTGCCCGCGGCGAGCGGCGGCCTGGGC
GCCGCGCTCGGCTCGATCTCGCGCAGCGGCGGACGCGCGCTGCGCGAAGCGCTCGGCGGCGCACCCGAGCAAGGCAGGCT
CACGCTCGAGCGCGACGCCGCGCTGCGCGACGCGCTTGTCGACTACGAGCGGCGCGTCGCCGCGCTCGCCGCCGACGCGC
TTGCGGGCCCCGGCGCCGCGTACCGGCTCGCGGCCGATTTCCACGGCTTCGGCGTCGATCCGTCCGTCCAGGCTTCCGCG
ATGCGCGCGGCCGACAGCGCGCTGCGCGACGTCAAGCGGCTCGCGGGCGATCGCGACGTCGGCGGCGACGTCGTCTGGAG
CCTCGTCGGCGGCCCGCTGCGCGCGCTGATCGCGTATGTCGAGCGGCAGGCGTCGTGCGCGCTGCAGGACGACTGGGAGC
GCGACGTGCTGTGGCCGCTCAGGCGCGCGGCGACGCGCGACGACGTGGAGGGTCTGCTGTACGGCCCGCAAGGCGCGATC
TGGGCGTTCGTCGACGGCCCGGCGAAGCCGTTCGTGCGTGTCGGCGCGGCGCGTGCATCGGCGCTCGACACGCTCGGCTA
CCGGCTGCCGTTCACCGACGCGTTCCTGCCGCTCATCGACGACGCGGCCGCCCGGCGCGTCGCGCAAGCGCGGCGCGACG
CCGAGCGGCGCGCGCAACAGCAGGCGGCGCTCGAGCTGGACGAGCGGATCGCATCGCTCGGCAAGCAGATCGACGCGCTG
CGCGCGCAAACGGTGCGCTTCGGGATCGTCGCGCAGCCGACCGACGTGAACCCGGATGCGCAGGCCAAGCCGTTCGAAAC
CGTGCTGACGCTGCAATGCGCGCCGCAGGCGCGCACGCTGACGAACTACAACCTGCGCGTGTCCGAGCAGATCGACTGGC
AACCCGATCGATGCGGCGACGCGACGCTGCGCATCTCGCTCGGCGGCGTCACGCTCGTGCGCCGTTACGCGGGATCGCTC
GGCGTCGCGCGCTTCGTCCAGGACTTCCGCTACGGCGTGCGCCGCTTCACGCCCCGCGATTTCCCGGACGCGAAAGCGCA
GCTTCAACGTCTCGGCGTGCGCCACATCGACGTGCGTTACGACTTCTCCGGGCATGACGCGCTGCTCGCGCACGTCGGTC
GGATCGACGCGCTCGAACGCGCGCGCCGCGACGACCTCGCGCGGCAGCGGCGCGCCGCGAACCGGCAGGACGACGACGCG
GGCGGCGCGACGGCGATCGCGCGGGCGGCGGCGTCCGTCGCGAATGCGCCGGGCGCCGCGCTGCCCCGCCGCATCGGCGT
GTGTTGGGGGGACCCGGCGCACGACAGGCCCGATGGCGACGGCGCGCAACCGTAA