Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   ABFU18_RS08315 Genome accession   NZ_CP155930
Coordinates   1961601..1961705 (-) Length   34 a.a.
NCBI ID   WP_259739915.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain CFBP 4954     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1953408..1976399 1961601..1961705 within 0
IScluster/Tn 1960408..1961540 1961601..1961705 flank 61


Gene organization within MGE regions


Location: 1953408..1976399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU18_RS08275 (ABFU18_08280) pilV 1953408..1953881 (+) 474 WP_011037628.1 type IV pilus modification protein PilV -
  ABFU18_RS08280 (ABFU18_08285) - 1953885..1955039 (+) 1155 WP_011037627.1 PilW family protein -
  ABFU18_RS08285 (ABFU18_08290) - 1955042..1955560 (+) 519 WP_011037626.1 PilX N-terminal domain-containing pilus assembly protein -
  ABFU18_RS08290 (ABFU18_08295) - 1955573..1959301 (+) 3729 WP_075287462.1 pilus assembly protein -
  ABFU18_RS08295 (ABFU18_08300) - 1959388..1959744 (+) 357 WP_230309684.1 type IV pilin protein -
  ABFU18_RS08305 (ABFU18_08310) - 1959925..1960296 (-) 372 WP_349745497.1 GspH/FimT family protein -
  ABFU18_RS08315 (ABFU18_08320) pilA 1961601..1961705 (-) 105 WP_259739915.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ABFU18_RS08320 (ABFU18_08325) uvrB 1961845..1963866 (+) 2022 WP_057672119.1 excinuclease ABC subunit UvrB -
  ABFU18_RS08330 (ABFU18_08335) - 1964441..1964683 (+) 243 WP_011269633.1 hypothetical protein -
  ABFU18_RS08335 (ABFU18_08340) - 1964717..1966390 (+) 1674 WP_011037620.1 type IV secretory system conjugative DNA transfer family protein -
  ABFU18_RS08340 (ABFU18_08345) - 1966719..1967129 (+) 411 WP_068574839.1 TcpQ domain-containing protein -
  ABFU18_RS08345 (ABFU18_08350) - 1967255..1968283 (+) 1029 WP_019237536.1 virB8 family protein -
  ABFU18_RS08350 (ABFU18_08355) - 1968280..1969047 (+) 768 WP_011037617.1 TrbG/VirB9 family P-type conjugative transfer protein -
  ABFU18_RS08355 (ABFU18_08360) - 1969044..1970225 (+) 1182 WP_011037616.1 TrbI/VirB10 family protein -
  ABFU18_RS08360 (ABFU18_08365) virB11 1970240..1971280 (+) 1041 WP_029628928.1 P-type DNA transfer ATPase VirB11 -
  ABFU18_RS08365 (ABFU18_08370) - 1971338..1972061 (+) 724 Protein_1624 lytic transglycosylase domain-containing protein -
  ABFU18_RS08370 (ABFU18_08375) - 1972222..1972626 (+) 405 WP_011037613.1 TrbC/VirB2 family protein -
  ABFU18_RS08375 (ABFU18_08380) - 1972619..1972930 (+) 312 WP_011037612.1 type IV secretion system protein VirB3 -
  ABFU18_RS08380 (ABFU18_08385) - 1973038..1975491 (+) 2454 WP_011037611.1 VirB4 family type IV secretion/conjugal transfer ATPase -
  ABFU18_RS08385 (ABFU18_08390) - 1975641..1976002 (-) 362 Protein_1628 integrase core domain-containing protein -

Sequence


Protein


Download         Length: 34 a.a.        Molecular weight: 3427.12 Da        Isoelectric Point: 5.8607

>NTDB_id=999813 ABFU18_RS08315 WP_259739915.1 1961601..1961705(-) (pilA) [Xanthomonas campestris pv. campestris strain CFBP 4954]
MQTGPQSPAKGYTATELLIVMAVLGLLAAIALPS

Nucleotide


Download         Length: 105 bp        

>NTDB_id=999813 ABFU18_RS08315 WP_259739915.1 1961601..1961705(-) (pilA) [Xanthomonas campestris pv. campestris strain CFBP 4954]
ATGCAGACAGGACCTCAGTCACCCGCAAAAGGGTACACGGCAACCGAGCTACTCATCGTGATGGCAGTGCTCGGCCTGCT
TGCCGCAATTGCGCTGCCGAGCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae C6706

51.515

97.059

0.5

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

51.515

97.059

0.5

  pilA Vibrio cholerae strain A1552

51.515

97.059

0.5

  pilA Vibrio campbellii strain DS40M4

47.059

100

0.471

  pilA Vibrio parahaemolyticus RIMD 2210633

44.118

100

0.441

  pilH Neisseria gonorrhoeae MS11

60

73.529

0.441

  pilE Neisseria gonorrhoeae MS11

60

73.529

0.441

  pilE Neisseria gonorrhoeae strain FA1090

60

73.529

0.441

  pilA Ralstonia pseudosolanacearum GMI1000

60

73.529

0.441

  pilA/pilA1 Synechocystis sp. PCC 6803

58.333

70.588

0.412

  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.828

85.294

0.382

  ctsG Campylobacter jejuni subsp. jejuni 81-176

54.167

70.588

0.382

  pilL Neisseria gonorrhoeae MS11

52

73.529

0.382

  pilX Neisseria meningitidis 8013

52

73.529

0.382


Multiple sequence alignment