Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   R8613_RS10240 Genome accession   NZ_AP026919
Coordinates   1947490..1948431 (-) Length   313 a.a.
NCBI ID   WP_001864563.1    Uniprot ID   A0A0T7STT8
Organism   Streptococcus pneumoniae strain PZ900700012     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1942490..1953431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8613_RS10200 (PC0012_19670) comYH 1942984..1943937 (-) 954 WP_050072683.1 class I SAM-dependent methyltransferase Machinery gene
  R8613_RS10205 (PC0012_19680) - 1943998..1944585 (-) 588 WP_000679772.1 class I SAM-dependent methyltransferase -
  R8613_RS10210 (PC0012_19690) comGG/cglG 1944688..1945134 (-) 447 WP_001811371.1 competence type IV pilus minor pilin ComGG Machinery gene
  R8613_RS10215 comGF/cglF 1945112..1945573 (-) 462 WP_000250539.1 competence type IV pilus minor pilin ComGF Machinery gene
  R8613_RS10220 (PC0012_19710) comGE/cglE 1945536..1945838 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R8613_RS10225 (PC0012_19720) comGD/cglD 1945801..1946205 (-) 405 WP_000588030.1 competence type IV pilus minor pilin ComGD Machinery gene
  R8613_RS10230 (PC0012_19730) comGC/cglC 1946198..1946524 (-) 327 WP_219565693.1 competence type IV pilus major pilin ComGC Machinery gene
  R8613_RS10235 (PC0012_19740) comGB/cglB 1946526..1947542 (-) 1017 WP_078132972.1 competence type IV pilus assembly protein ComGB Machinery gene
  R8613_RS10240 (PC0012_19750) comGA/cglA/cilD 1947490..1948431 (-) 942 WP_001864563.1 competence type IV pilus ATPase ComGA Machinery gene
  R8613_RS10245 (PC0012_19760) - 1948507..1948872 (-) 366 WP_000286415.1 DUF1033 family protein -
  R8613_RS10250 (PC0012_19770) - 1949023..1950081 (-) 1059 WP_044812807.1 zinc-dependent alcohol dehydrogenase family protein -
  R8613_RS10255 (PC0012_19780) nagA 1950244..1951395 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  R8613_RS10260 (PC0012_19790) - 1951548..1953365 (-) 1818 WP_001220858.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35565.43 Da        Isoelectric Point: 5.6206

>NTDB_id=97911 R8613_RS10240 WP_001864563.1 1947490..1948431(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain PZ900700012]
MVQEIAQEIIRSAREKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=97911 R8613_RS10240 WP_001864563.1 1947490..1948431(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain PZ900700012]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGGAAAAAGGGACGCAGGATATTTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAACAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T7STT8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.042

100

0.99
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.166

100

0.962
  comYA Streptococcus gordonii str. Challis substr. CH1

78.387

99.042

0.776
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55

Multiple sequence alignment