Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   R8613_RS10235 Genome accession   NZ_AP026919
Coordinates   1946526..1947542 (-) Length   338 a.a.
NCBI ID   WP_078132972.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain PZ900700012     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1941526..1952542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8613_RS10190 - 1941611..1941742 (-) 132 WP_000768904.1 hypothetical protein -
  R8613_RS10195 (PC0012_19660) - 1941743..1942933 (-) 1191 WP_000167769.1 acetate kinase -
  R8613_RS10200 (PC0012_19670) comYH 1942984..1943937 (-) 954 WP_050072683.1 class I SAM-dependent methyltransferase Machinery gene
  R8613_RS10205 (PC0012_19680) - 1943998..1944585 (-) 588 WP_000679772.1 class I SAM-dependent methyltransferase -
  R8613_RS10210 (PC0012_19690) comGG/cglG 1944688..1945134 (-) 447 WP_001811371.1 competence type IV pilus minor pilin ComGG Machinery gene
  R8613_RS10215 comGF/cglF 1945112..1945573 (-) 462 WP_000250539.1 competence type IV pilus minor pilin ComGF Machinery gene
  R8613_RS10220 (PC0012_19710) comGE/cglE 1945536..1945838 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R8613_RS10225 (PC0012_19720) comGD/cglD 1945801..1946205 (-) 405 WP_000588030.1 competence type IV pilus minor pilin ComGD Machinery gene
  R8613_RS10230 (PC0012_19730) comGC/cglC 1946198..1946524 (-) 327 WP_219565693.1 competence type IV pilus major pilin ComGC Machinery gene
  R8613_RS10235 (PC0012_19740) comGB/cglB 1946526..1947542 (-) 1017 WP_078132972.1 competence type IV pilus assembly protein ComGB Machinery gene
  R8613_RS10240 (PC0012_19750) comGA/cglA/cilD 1947490..1948431 (-) 942 WP_001864563.1 competence type IV pilus ATPase ComGA Machinery gene
  R8613_RS10245 (PC0012_19760) - 1948507..1948872 (-) 366 WP_000286415.1 DUF1033 family protein -
  R8613_RS10250 (PC0012_19770) - 1949023..1950081 (-) 1059 WP_044812807.1 zinc-dependent alcohol dehydrogenase family protein -
  R8613_RS10255 (PC0012_19780) nagA 1950244..1951395 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38406.42 Da        Isoelectric Point: 9.4802

>NTDB_id=97910 R8613_RS10235 WP_078132972.1 1946526..1947542(-) (comGB/cglB) [Streptococcus pneumoniae strain PZ900700012]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=97910 R8613_RS10235 WP_078132972.1 1946526..1947542(-) (comGB/cglB) [Streptococcus pneumoniae strain PZ900700012]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCTAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGTTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

99.704

100

0.997
  comGB/cglB Streptococcus pneumoniae D39

99.704

100

0.997
  comGB/cglB Streptococcus pneumoniae R6

99.704

100

0.997
  comGB/cglB Streptococcus pneumoniae TIGR4

99.704

100

0.997
  comGB/cglB Streptococcus mitis SK321

95.562

100

0.956
  comGB/cglB Streptococcus mitis NCTC 12261

94.97

100

0.95
  comYB Streptococcus gordonii str. Challis substr. CH1

71.429

99.408

0.71
  comYB Streptococcus mutans UA140

57.862

94.083

0.544
  comYB Streptococcus mutans UA159

57.862

94.083

0.544
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment