Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   R8522_RS10460 Genome accession   NZ_AP026916
Coordinates   2022388..2023329 (-) Length   313 a.a.
NCBI ID   WP_000249549.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain PZ900700006     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2017388..2028329
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8522_RS10420 (PC0006_20810) comYH 2017881..2018834 (-) 954 WP_000345123.1 class I SAM-dependent methyltransferase Machinery gene
  R8522_RS10425 (PC0006_20820) - 2018895..2019482 (-) 588 WP_000679774.1 class I SAM-dependent methyltransferase -
  R8522_RS10430 (PC0006_20830) comGG/cglG 2019619..2020032 (-) 414 WP_000265630.1 competence type IV pilus minor pilin ComGG Machinery gene
  R8522_RS10435 comGF/cglF 2020010..2020471 (-) 462 WP_000250548.1 competence type IV pilus minor pilin ComGF Machinery gene
  R8522_RS10440 (PC0006_20850) comGE/cglE 2020434..2020736 (-) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  R8522_RS10445 (PC0006_20860) comGD/cglD 2020699..2021103 (-) 405 WP_000588007.1 competence type IV pilus minor pilin ComGD Machinery gene
  R8522_RS10450 (PC0006_20870) comGC/cglC 2021096..2021422 (-) 327 WP_000738624.1 competence type IV pilus major pilin ComGC Machinery gene
  R8522_RS10455 (PC0006_20880) comGB/cglB 2021424..2022440 (-) 1017 WP_078140087.1 competence type IV pilus assembly protein ComGB Machinery gene
  R8522_RS10460 (PC0006_20890) comGA/cglA/cilD 2022388..2023329 (-) 942 WP_000249549.1 competence type IV pilus ATPase ComGA Machinery gene
  R8522_RS10465 (PC0006_20900) - 2023405..2023770 (-) 366 WP_000286415.1 DUF1033 family protein -
  R8522_RS10470 (PC0006_20910) - 2023921..2024979 (-) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -
  R8522_RS10475 (PC0006_20920) nagA 2025142..2026293 (-) 1152 WP_001134454.1 N-acetylglucosamine-6-phosphate deacetylase -
  R8522_RS10480 (PC0006_20930) - 2026446..2028263 (-) 1818 WP_001220869.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35543.45 Da        Isoelectric Point: 6.0083

>NTDB_id=97678 R8522_RS10460 WP_000249549.1 2022388..2023329(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain PZ900700006]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=97678 R8522_RS10460 WP_000249549.1 2022388..2023329(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain PZ900700006]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCTAAGTT
AGATGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.042

100

0.99
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.805

100

0.968
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364

Multiple sequence alignment