Detailed information NTG0097673

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: R8522_RS10435
• Genome accession: NZ_AP026916
• Coordinates: 2020010..2020471 (-)
• Length: 153 a.a.
• NCBI ID: WP_000250548.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain PZ900700006
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2015010..2025471

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R8522_RS10400 (PC0006_20780)	-	2015321..2016145 (-)	825	WP_209022237.1	membrane protein insertase YidC	-
R8522_RS10405 (PC0006_20790)	rnpA	2016120..2016491 (-)	372	WP_000739246.1	ribonuclease P protein component	-
R8522_RS10410	-	2016508..2016639 (-)	132	WP_000768904.1	hypothetical protein	-
R8522_RS10415 (PC0006_20800)	-	2016640..2017830 (-)	1191	WP_000167759.1	acetate kinase	-
R8522_RS10420 (PC0006_20810)	comYH	2017881..2018834 (-)	954	WP_000345123.1	class I SAM-dependent methyltransferase	Machinery gene
R8522_RS10425 (PC0006_20820)	-	2018895..2019482 (-)	588	WP_000679774.1	class I SAM-dependent methyltransferase	-
R8522_RS10430 (PC0006_20830)	comGG/cglG	2019619..2020032 (-)	414	WP_000265630.1	competence type IV pilus minor pilin ComGG	Machinery gene
R8522_RS10435	comGF/cglF	2020010..2020471 (-)	462	WP_000250548.1	competence type IV pilus minor pilin ComGF	Machinery gene
R8522_RS10440 (PC0006_20850)	comGE/cglE	2020434..2020736 (-)	303	WP_000413380.1	competence type IV pilus minor pilin ComGE	Machinery gene
R8522_RS10445 (PC0006_20860)	comGD/cglD	2020699..2021103 (-)	405	WP_000588007.1	competence type IV pilus minor pilin ComGD	Machinery gene
R8522_RS10450 (PC0006_20870)	comGC/cglC	2021096..2021422 (-)	327	WP_000738624.1	competence type IV pilus major pilin ComGC	Machinery gene
R8522_RS10455 (PC0006_20880)	comGB/cglB	2021424..2022440 (-)	1017	WP_078140087.1	competence type IV pilus assembly protein ComGB	Machinery gene
R8522_RS10460 (PC0006_20890)	comGA/cglA/cilD	2022388..2023329 (-)	942	WP_000249549.1	competence type IV pilus ATPase ComGA	Machinery gene
R8522_RS10465 (PC0006_20900)	-	2023405..2023770 (-)	366	WP_000286415.1	DUF1033 family protein	-
R8522_RS10470 (PC0006_20910)	-	2023921..2024979 (-)	1059	WP_000649471.1	zinc-dependent alcohol dehydrogenase family protein	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17851.34 Da
• Isoelectric Point: 6.4832

>NTDB_id=97673 R8522_RS10435 WP_000250548.1 2020010..2020471(-) (comGF/cglF) [Streptococcus pneumoniae strain PZ900700006]
MVQNSCWQSKSHKVKAFTLLESLLALLVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVESN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide

• Length: 462 bp

>NTDB_id=97673 R8522_RS10435 WP_000250548.1 2020010..2020471(-) (comGF/cglF) [Streptococcus pneumoniae strain PZ900700006]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTGCT
CGTCATCAGTGGAGGCTTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGATCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAAGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	93.464	100	0.935
comGF/cglF	Streptococcus pneumoniae D39	93.464	100	0.935
comGF/cglF	Streptococcus pneumoniae R6	93.464	100	0.935
comGF/cglF	Streptococcus pneumoniae TIGR4	93.464	100	0.935
comGF/cglF	Streptococcus mitis SK321	92.157	100	0.922
comGF/cglF	Streptococcus mitis NCTC 12261	90.85	100	0.909
comYF	Streptococcus mutans UA159	41.791	87.582	0.366
comYF	Streptococcus mutans UA140	41.791	87.582	0.366