Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   R8522_RS10455 Genome accession   NZ_AP026916
Coordinates   2021424..2022440 (-) Length   338 a.a.
NCBI ID   WP_078140087.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain PZ900700006     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2016424..2027440
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8522_RS10410 - 2016508..2016639 (-) 132 WP_000768904.1 hypothetical protein -
  R8522_RS10415 (PC0006_20800) - 2016640..2017830 (-) 1191 WP_000167759.1 acetate kinase -
  R8522_RS10420 (PC0006_20810) comYH 2017881..2018834 (-) 954 WP_000345123.1 class I SAM-dependent methyltransferase Machinery gene
  R8522_RS10425 (PC0006_20820) - 2018895..2019482 (-) 588 WP_000679774.1 class I SAM-dependent methyltransferase -
  R8522_RS10430 (PC0006_20830) comGG/cglG 2019619..2020032 (-) 414 WP_000265630.1 competence type IV pilus minor pilin ComGG Machinery gene
  R8522_RS10435 comGF/cglF 2020010..2020471 (-) 462 WP_000250548.1 competence type IV pilus minor pilin ComGF Machinery gene
  R8522_RS10440 (PC0006_20850) comGE/cglE 2020434..2020736 (-) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  R8522_RS10445 (PC0006_20860) comGD/cglD 2020699..2021103 (-) 405 WP_000588007.1 competence type IV pilus minor pilin ComGD Machinery gene
  R8522_RS10450 (PC0006_20870) comGC/cglC 2021096..2021422 (-) 327 WP_000738624.1 competence type IV pilus major pilin ComGC Machinery gene
  R8522_RS10455 (PC0006_20880) comGB/cglB 2021424..2022440 (-) 1017 WP_078140087.1 competence type IV pilus assembly protein ComGB Machinery gene
  R8522_RS10460 (PC0006_20890) comGA/cglA/cilD 2022388..2023329 (-) 942 WP_000249549.1 competence type IV pilus ATPase ComGA Machinery gene
  R8522_RS10465 (PC0006_20900) - 2023405..2023770 (-) 366 WP_000286415.1 DUF1033 family protein -
  R8522_RS10470 (PC0006_20910) - 2023921..2024979 (-) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -
  R8522_RS10475 (PC0006_20920) nagA 2025142..2026293 (-) 1152 WP_001134454.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38596.70 Da        Isoelectric Point: 9.7256

>NTDB_id=97677 R8522_RS10455 WP_078140087.1 2021424..2022440(-) (comGB/cglB) [Streptococcus pneumoniae strain PZ900700006]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVIQMRAGLSQGKSFSEMMESLGFSSTI
ITQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=97677 R8522_RS10455 WP_078140087.1 2021424..2022440(-) (comGB/cglB) [Streptococcus pneumoniae strain PZ900700006]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGATCCAGATGCGTGCGGGCTTGTCTCAAGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATTTTCAAGCACCATT
ATCACTCAGTTATCCCTAGCTGAAGTTCATGGAAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCTAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGTTTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTTTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

97.633

100

0.976
  comGB/cglB Streptococcus pneumoniae D39

97.633

100

0.976
  comGB/cglB Streptococcus pneumoniae R6

97.633

100

0.976
  comGB/cglB Streptococcus pneumoniae TIGR4

97.633

100

0.976
  comGB/cglB Streptococcus mitis SK321

94.379

100

0.944
  comGB/cglB Streptococcus mitis NCTC 12261

94.083

100

0.941
  comYB Streptococcus gordonii str. Challis substr. CH1

70.536

99.408

0.701
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment