Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WMB06_RS02780 Genome accession   NZ_CP151275
Coordinates   568189..569322 (+) Length   377 a.a.
NCBI ID   WP_341677555.1    Uniprot ID   -
Organism   Niveibacterium sp. SC-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 563189..574322
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WMB06_RS02750 (WMB06_02750) - 563681..564349 (+) 669 WP_341677549.1 NAD(P)-binding domain-containing protein -
  WMB06_RS02755 (WMB06_02755) - 564373..564672 (-) 300 WP_341677550.1 DUF167 domain-containing protein -
  WMB06_RS02760 (WMB06_02760) - 564686..565258 (-) 573 WP_341677551.1 YggT family protein -
  WMB06_RS02765 (WMB06_02765) proC 565466..566278 (-) 813 WP_341677552.1 pyrroline-5-carboxylate reductase -
  WMB06_RS02770 (WMB06_02770) - 566329..567030 (-) 702 WP_341677553.1 YggS family pyridoxal phosphate-dependent enzyme -
  WMB06_RS02775 (WMB06_02775) pilT 567114..568157 (+) 1044 WP_341677554.1 type IV pilus twitching motility protein PilT Machinery gene
  WMB06_RS02780 (WMB06_02780) pilU 568189..569322 (+) 1134 WP_341677555.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WMB06_RS02785 (WMB06_02785) - 569423..570085 (-) 663 WP_341677556.1 hypothetical protein -
  WMB06_RS02790 (WMB06_02790) - 570094..571083 (-) 990 WP_341677557.1 5'/3'-nucleotidase SurE -
  WMB06_RS02795 (WMB06_02795) - 571204..571803 (+) 600 WP_341677558.1 TetR/AcrR family transcriptional regulator -
  WMB06_RS02800 (WMB06_02800) - 571822..573522 (-) 1701 WP_341677560.1 FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42308.79 Da        Isoelectric Point: 6.5882

>NTDB_id=976045 WMB06_RS02780 WP_341677555.1 568189..569322(+) (pilU) [Niveibacterium sp. SC-1]
MERDQALKFMHDLLKLMLSKRGSDLFITAGFPPAIKIDGKIIPQSNQVLTPQHTAELVRAVMNDRQAAEFESTKECNFAI
SPAGIGRFRCNAFVQQSRIGLVCRTIAQKIPTLEELGMPPVLKDIALAKRGLVIFVGGTGTGKTTSLAALVDYRNENSYG
HIITLEDPIEYVHAHKNCVVTQREVGIDTDTWEAGLKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQAIDRIINFFPEERRHQLLMDLSLNLRGMISQRLIPLKDRKGRVPAVEIMLNSPLMADLIFKGEVAELKDLMKRSRELG
MQTFDQSLFDIYENNLISYEDALRNADSINDLRLQIKLNSKHSDRDLNAGIQNLDIV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=976045 WMB06_RS02780 WP_341677555.1 568189..569322(+) (pilU) [Niveibacterium sp. SC-1]
ATGGAACGGGACCAGGCACTCAAGTTCATGCACGACCTGCTCAAGCTGATGCTGAGCAAGCGTGGGTCAGACCTCTTCAT
CACGGCCGGTTTTCCGCCCGCGATCAAGATCGACGGCAAGATCATTCCGCAGTCCAATCAGGTGCTCACGCCGCAGCACA
CCGCCGAGCTGGTGCGCGCGGTGATGAACGACCGCCAGGCGGCGGAATTCGAGTCCACCAAGGAGTGTAATTTCGCGATC
TCGCCCGCGGGCATCGGTCGCTTCCGCTGCAATGCCTTCGTGCAGCAGAGCCGCATCGGCCTCGTGTGCCGGACCATCGC
GCAGAAGATCCCCACGCTGGAAGAGCTCGGCATGCCTCCGGTGTTGAAGGACATCGCGCTCGCCAAGCGCGGCCTGGTGA
TCTTCGTCGGCGGTACCGGCACCGGCAAGACGACCTCGCTCGCCGCGCTGGTCGACTACCGCAACGAGAACAGCTACGGC
CACATCATCACGCTGGAAGATCCGATCGAATACGTCCACGCGCACAAGAACTGCGTGGTGACCCAGCGCGAGGTCGGGAT
CGACACCGATACTTGGGAAGCGGGCCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGTGAAATCCGCG
ACCGCGAGACCATGGACTATGCGATCGCCTTCGCCGAGACCGGCCACCTGTGTCTCGCCACCCTGCACGCGAACAGCGCC
AACCAGGCGATCGACCGGATCATCAACTTCTTCCCGGAAGAACGTCGTCACCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCGGCATGATCTCGCAGCGCCTGATCCCGCTGAAGGACCGCAAGGGTCGGGTGCCGGCGGTGGAGATCATGCTCAACT
CGCCGCTGATGGCCGACCTGATCTTCAAGGGCGAGGTCGCCGAGCTCAAGGACCTGATGAAGCGCTCGCGCGAACTGGGC
ATGCAGACCTTCGACCAGTCGCTCTTCGACATCTACGAGAACAATCTCATCAGCTACGAAGACGCGCTGCGCAACGCCGA
CTCGATCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCAAGCACTCGGATCGCGACCTGAACGCCGGCATCCAGAACC
TGGATATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(126-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.363

96.552

0.602
  pilU Acinetobacter baylyi ADP1

57.265

93.103

0.533
  pilU Vibrio cholerae strain A1552

53.889

95.491

0.515
  pilT Pseudomonas aeruginosa PAK

43.284

88.859

0.385
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.09

88.859

0.374
  pilT Acinetobacter baylyi ADP1

41.543

89.39

0.371
  pilT Pseudomonas stutzeri DSM 10701

41.791

88.859

0.371
  pilT Legionella pneumophila strain Lp02

42.296

87.798

0.371
  pilT Acinetobacter baumannii strain A118

42.296

87.798

0.371
  pilT Acinetobacter baumannii D1279779

42.296

87.798

0.371
  pilT Legionella pneumophila strain ERS1305867

42.296

87.798

0.371
  pilT Acinetobacter nosocomialis M2

42.296

87.798

0.371

Multiple sequence alignment