Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OO995_RS05910 Genome accession   NZ_AP026732
Coordinates   1345190..1346302 (+) Length   370 a.a.
NCBI ID   WP_037429434.1    Uniprot ID   A0AAE4TNT5
Organism   Shewanella xiamenensis strain NUITM-VS2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1340190..1351302
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO995_RS05870 (NUITMVS2_11260) rdgB 1340202..1340819 (-) 618 WP_037420793.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  OO995_RS05875 (NUITMVS2_11270) - 1340934..1341368 (-) 435 WP_037429443.1 DUF4426 domain-containing protein -
  OO995_RS05880 (NUITMVS2_11280) yggU 1341437..1341727 (-) 291 WP_011621978.1 DUF167 family protein YggU -
  OO995_RS05885 (NUITMVS2_11290) - 1341727..1342275 (-) 549 WP_023266519.1 YggT family protein -
  OO995_RS05890 (NUITMVS2_11300) proC 1342351..1343169 (-) 819 WP_037429440.1 pyrroline-5-carboxylate reductase -
  OO995_RS05895 - 1343266..1343394 (+) 129 WP_264889417.1 hypothetical protein -
  OO995_RS05900 (NUITMVS2_11310) - 1343391..1344107 (-) 717 WP_037429438.1 YggS family pyridoxal phosphate-dependent enzyme -
  OO995_RS05905 (NUITMVS2_11320) pilT 1344143..1345180 (+) 1038 WP_023266515.1 type IV pilus twitching motility protein PilT Machinery gene
  OO995_RS05910 (NUITMVS2_11330) pilU 1345190..1346302 (+) 1113 WP_037429434.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OO995_RS05915 (NUITMVS2_11340) - 1346474..1347007 (+) 534 WP_037429431.1 glutathione peroxidase -
  OO995_RS05920 (NUITMVS2_11350) hemH 1347076..1348059 (+) 984 WP_086024055.1 ferrochelatase -
  OO995_RS05925 (NUITMVS2_11360) ruvX 1348120..1348545 (-) 426 WP_055647823.1 Holliday junction resolvase RuvX -
  OO995_RS05930 (NUITMVS2_11370) - 1348575..1349138 (-) 564 WP_037429424.1 YqgE/AlgH family protein -
  OO995_RS05935 (NUITMVS2_11380) yciH 1349207..1349536 (-) 330 WP_037420757.1 stress response translation initiation inhibitor YciH -
  OO995_RS05940 (NUITMVS2_11390) - 1349699..1350013 (-) 315 WP_037420754.1 hypothetical protein -
  OO995_RS05945 (NUITMVS2_11400) - 1350192..1350752 (+) 561 WP_037420751.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41131.35 Da        Isoelectric Point: 6.3728

>NTDB_id=96752 OO995_RS05910 WP_037429434.1 1345190..1346302(+) (pilU) [Shewanella xiamenensis strain NUITM-VS2]
MDVRPFLKVMVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESVMTEAQKKEFHTTRECNFAFAVKELGR
FRVSAFWQRESPGCVMRRIETKIPEVEDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVAKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLQLYVDGEISYADALHHADSPNDLRLMIKLQNKEPASSSFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=96752 OO995_RS05910 WP_037429434.1 1345190..1346302(+) (pilU) [Shewanella xiamenensis strain NUITM-VS2]
ATGGATGTTCGTCCGTTTTTAAAAGTCATGGTAGAGCGTAAGGCCTCTGACTTGTTTATTACCGCAGGTTTTCCGCCGAG
TGCCAAAATTGATGGTGAGTTACGCCCATTAGCCGAGAGTGCCTTCACGCCCGCGCAGTCTTTGGATTTTGTCGAGTCCG
TGATGACCGAGGCACAAAAGAAGGAGTTTCACACGACTCGCGAGTGTAACTTTGCTTTTGCAGTGAAGGAGTTAGGTCGT
TTTCGCGTGAGTGCATTCTGGCAGCGTGAATCTCCAGGATGCGTGATGCGCCGGATTGAGACCAAAATCCCTGAGGTGGA
AGACTTAAAACTGCCACCGATTTTAAAAGATCTGGTGATGAGTAAACGTGGTCTTATCATCATGGTTGGGGGAACGGGAA
CCGGTAAGTCGACCTCCTTGGCGGCGTTAGTGGGTTATCGTAATGCCCATGCCCGTGGTCATATCCTCACCATCGAAGAC
CCGGTAGAATTTGTGCACGACCATCGCAAAAGCATCATCACTCAACGTGAAGTGGGGATAGATACAGAGTCCTTTGATGC
GGCGCTGAAGAGTTCGCTGCGTCAGGCGCCCGATGTGATTTTGATTGGTGAGATCCGTACTCAAGAAACCATGGAGTTTG
CGCTTTCCTTCGCCGAAACGGGTCACCTCTGTATGGCGACACTGCATGCTAACAATGCTAACCAAGCATTAGACCGGATT
ATGCACTTAGTGCCAGAGAGTAAACATCAACAGCTATTATTCGATTTGTCACTCAACCTGCGCGGTATTGTGGCGCAGCA
GCTGGTGGCTAAAGTTGATGGCACAGGGCGCCGGGCGGCGATTGAAGTCTTGATCAATACGCCACGTGTCGCGAGTTTGA
TTGCTAAAAACGAGCTGCATTTGCTCAAAGAAACCATGGCCAAATCGAATGAGCAGGGCATGCAGACCTTTGACCAAGCA
CTGTTACAGCTCTATGTCGATGGGGAGATCAGTTATGCCGACGCGCTTCACCATGCTGACTCACCCAACGACTTGCGGTT
AATGATTAAATTGCAAAATAAAGAACCCGCCAGTTCGAGCTTTATGGAAGGCGTGACCTTGGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.377

100

0.605
  pilU Vibrio cholerae strain A1552

59.189

100

0.592
  pilU Acinetobacter baylyi ADP1

60.511

95.135

0.576
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.598

88.649

0.386
  pilT Vibrio cholerae strain A1552

43.598

88.649

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.29

93.243

0.376
  pilT Legionella pneumophila strain Lp02

41.088

89.459

0.368
  pilT Legionella pneumophila strain ERS1305867

41.088

89.459

0.368
  pilT Acinetobacter baumannii D1279779

39.766

92.432

0.368
  pilT Acinetobacter baumannii strain A118

39.766

92.432

0.368
  pilT Acinetobacter nosocomialis M2

39.766

92.432

0.368
  pilT Acinetobacter baylyi ADP1

39.474

92.432

0.365

Multiple sequence alignment