Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OO995_RS05905 Genome accession   NZ_AP026732
Coordinates   1344143..1345180 (+) Length   345 a.a.
NCBI ID   WP_023266515.1    Uniprot ID   A0A5B8QWG8
Organism   Shewanella xiamenensis strain NUITM-VS2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1339143..1350180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO995_RS05870 (NUITMVS2_11260) rdgB 1340202..1340819 (-) 618 WP_037420793.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  OO995_RS05875 (NUITMVS2_11270) - 1340934..1341368 (-) 435 WP_037429443.1 DUF4426 domain-containing protein -
  OO995_RS05880 (NUITMVS2_11280) yggU 1341437..1341727 (-) 291 WP_011621978.1 DUF167 family protein YggU -
  OO995_RS05885 (NUITMVS2_11290) - 1341727..1342275 (-) 549 WP_023266519.1 YggT family protein -
  OO995_RS05890 (NUITMVS2_11300) proC 1342351..1343169 (-) 819 WP_037429440.1 pyrroline-5-carboxylate reductase -
  OO995_RS05895 - 1343266..1343394 (+) 129 WP_264889417.1 hypothetical protein -
  OO995_RS05900 (NUITMVS2_11310) - 1343391..1344107 (-) 717 WP_037429438.1 YggS family pyridoxal phosphate-dependent enzyme -
  OO995_RS05905 (NUITMVS2_11320) pilT 1344143..1345180 (+) 1038 WP_023266515.1 type IV pilus twitching motility protein PilT Machinery gene
  OO995_RS05910 (NUITMVS2_11330) pilU 1345190..1346302 (+) 1113 WP_037429434.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OO995_RS05915 (NUITMVS2_11340) - 1346474..1347007 (+) 534 WP_037429431.1 glutathione peroxidase -
  OO995_RS05920 (NUITMVS2_11350) hemH 1347076..1348059 (+) 984 WP_086024055.1 ferrochelatase -
  OO995_RS05925 (NUITMVS2_11360) ruvX 1348120..1348545 (-) 426 WP_055647823.1 Holliday junction resolvase RuvX -
  OO995_RS05930 (NUITMVS2_11370) - 1348575..1349138 (-) 564 WP_037429424.1 YqgE/AlgH family protein -
  OO995_RS05935 (NUITMVS2_11380) yciH 1349207..1349536 (-) 330 WP_037420757.1 stress response translation initiation inhibitor YciH -
  OO995_RS05940 (NUITMVS2_11390) - 1349699..1350013 (-) 315 WP_037420754.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38310.00 Da        Isoelectric Point: 6.9096

>NTDB_id=96751 OO995_RS05905 WP_023266515.1 1344143..1345180(+) (pilT) [Shewanella xiamenensis strain NUITM-VS2]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDFEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=96751 OO995_RS05905 WP_023266515.1 1344143..1345180(+) (pilT) [Shewanella xiamenensis strain NUITM-VS2]
ATGGAAATCACTGAGTTATTAGCCTTTAGTGTAAAACACAAAGCCTCGGATCTACACCTCTCTGCAGGGATATCTCCCAT
GATCCGTGTCGACGGTGAAGTGAGAAAAATTAACCTGCCCGCGCTCGATCATCAGGGTGTACACAGCCTTGTGTACGACA
TTATGAATGATAAACAGCGTAAGGACTTTGAAGAGCATTTAGAAATCGACTTTTCGTTCGAAGTCCCTAATTTAGCGCGT
TTCCGTGTGAACGCTTTTAACCAATCCCGCGGCGCGGCGGCGGTATTTCGTACCATTCCTAGTGATATATTGTCACTTGA
GCAGCTAGGTGCGCCTGAAATTTTTAAAAAAATCGCCAGTTTTCCCCGTGGCTTAGTGTTAGTCACTGGCCCTACTGGTT
CGGGTAAGAGTACCACACTTGCGGCCATGGTGGATTACATCAATGAGAACCGCCACGACCATATCTTAACCATTGAAGAT
CCTATCGAATTCGTTCACCAGAATAAGCAATGTTTGATTAACCAACGGGAAGTGCATCGTCATACCCACAGCTTTAACGC
GGCGCTACGTAGTGCACTGCGTGAAGACCCTGACGTTATCCTCGTCGGTGAGATGCGTGACCTTGAAACCATTCGTCTGG
CGATGACGGCTGCCGAAACGGGCCACTTAGTTTTTGGTACCTTGCACACCACCTCGGCGGCTAAGACCATCGACCGTGTG
GTTGACGTGTTCCCTGCCGGTGAAAAGGACATGGTGCGTACCATGTTGTCTGAATCATTACAGGCGGTTATTTCGCAAAC
TCTGATTAAGAAAATCGGTGGTGGCCGTGTGGCTGCCCACGAAATTATGATGGGAACGCCCGCCATCCGTAACCTTATCC
GTGAAGATAAAGTGGCGCAGATGTACTCAGCTATTCAAACGGGGATGGCCCATGGCATGCAAACGCTTGAACAGTGTCTG
CAAAACTTAGTCAACCGTGGTCTCATCACCCGTGAGGATGCCATGGCGAAGAGTTCAAATAAACAAGCGACATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B8QWG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

80.409

99.13

0.797
  pilT Acinetobacter baumannii strain A118

80.409

99.13

0.797
  pilT Acinetobacter nosocomialis M2

80.409

99.13

0.797
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Acinetobacter baylyi ADP1

77.843

99.42

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.681

0.707
  pilT Vibrio cholerae strain A1552

72.404

97.681

0.707
  pilT Neisseria meningitidis 8013

69.767

99.71

0.696
  pilT Neisseria gonorrhoeae MS11

69.477

99.71

0.693
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.261

0.493
  pilU Pseudomonas stutzeri DSM 10701

42.274

99.42

0.42
  pilU Vibrio cholerae strain A1552

41.691

99.42

0.414
  pilU Acinetobacter baylyi ADP1

40.059

97.681

0.391

Multiple sequence alignment