Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   NYE79_RS01035 Genome accession   NZ_CP150168
Coordinates   181626..182567 (+) Length   313 a.a.
NCBI ID   WP_216728920.1    Uniprot ID   -
Organism   Streptococcus sp. FSL W8-0197     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 176626..187567
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYE79_RS01015 (NYE79_01015) tgt 176656..177798 (+) 1143 WP_001285237.1 tRNA guanosine(34) transglycosylase Tgt -
  NYE79_RS01020 (NYE79_01020) - 177932..179749 (+) 1818 WP_216728917.1 acyltransferase family protein -
  NYE79_RS01025 (NYE79_01025) nagA 179890..181041 (+) 1152 WP_216728918.1 N-acetylglucosamine-6-phosphate deacetylase -
  NYE79_RS01030 (NYE79_01030) - 181196..181561 (+) 366 WP_216728919.1 DUF1033 family protein -
  NYE79_RS01035 (NYE79_01035) comGA/cglA/cilD 181626..182567 (+) 942 WP_216728920.1 competence type IV pilus ATPase ComGA Machinery gene
  NYE79_RS01040 (NYE79_01040) comGB/cglB 182515..183531 (+) 1017 WP_216728921.1 competence type IV pilus assembly protein ComGB Machinery gene
  NYE79_RS01045 (NYE79_01045) comGC/cglC 183533..183856 (+) 324 WP_000735800.1 competence type IV pilus major pilin ComGC Machinery gene
  NYE79_RS01050 (NYE79_01050) comGD/cglD 183819..184253 (+) 435 WP_216728922.1 competence type IV pilus minor pilin ComGD Machinery gene
  NYE79_RS01055 (NYE79_01055) comGE/cglE 184216..184518 (+) 303 WP_200370534.1 competence type IV pilus minor pilin ComGE Machinery gene
  NYE79_RS01060 (NYE79_01060) comGF/cglF 184481..184942 (+) 462 WP_070568120.1 competence type IV pilus minor pilin ComGF Machinery gene
  NYE79_RS01065 (NYE79_01065) comGG/cglG 184920..185333 (+) 414 WP_070568123.1 competence type IV pilus minor pilin ComGG Machinery gene
  NYE79_RS01070 (NYE79_01070) - 185366..185956 (+) 591 WP_216728923.1 class I SAM-dependent methyltransferase -
  NYE79_RS01075 (NYE79_01075) comYH 186015..186968 (+) 954 WP_216728924.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35506.42 Da        Isoelectric Point: 5.2695

>NTDB_id=964578 NYE79_RS01035 WP_216728920.1 181626..182567(+) (comGA/cglA/cilD) [Streptococcus sp. FSL W8-0197]
MVQEIAQKIIATAKEKKAQDIYFIPKEKSYELHMRVGDERCLVDSYEFDVLAAVISHFKFVAGMNVGEKRRSQLGSCDYQ
HGEKVSSLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDMNELGEQYRQRGLYLFAGPVGSGKTTLMHELAKSLFMGQQ
VMSIEDPVEIKQEDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVTEEELAVVLQGVCYQRLIGGGGIVDFANKDFQEHQPTSWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=964578 NYE79_RS01035 WP_216728920.1 181626..182567(+) (comGA/cglA/cilD) [Streptococcus sp. FSL W8-0197]
ATGGTACAAGAAATTGCACAGAAAATTATTGCTACTGCGAAAGAAAAGAAGGCTCAGGATATCTATTTTATTCCCAAGGA
AAAATCCTATGAGCTTCACATGCGGGTTGGAGACGAGCGGTGTCTCGTTGACTCCTATGAGTTTGATGTTTTAGCTGCTG
TGATTAGTCATTTTAAGTTTGTAGCGGGTATGAATGTAGGGGAGAAGAGACGTAGTCAGCTGGGCTCTTGCGACTATCAG
CATGGGGAGAAGGTGTCTTCTCTTCGTTTGTCTACCGTAGGAGATTATCGGGGACATGAGAGTTTGGTTATTCGTTTGTT
GCACGACGAAGAGCAGGACCTGCATTTCTGGTTTCAGGATATGAACGAACTGGGTGAACAGTACCGGCAACGGGGGCTCT
ATCTCTTTGCAGGTCCAGTTGGAAGTGGCAAGACGACTCTGATGCACGAATTAGCCAAGTCTCTGTTTATGGGGCAACAG
GTTATGTCCATTGAAGATCCTGTCGAGATCAAGCAGGAAGACATGCTCCAGTTGCAGTTGAATGAGGCGATTGGCTTGAC
CTATGAAAATCTGATCAAACTGTCTCTCCGGCATCGTCCGGACCTCTTGATTATCGGTGAAATTCGTGATAGCGAGACGG
CGCGTGCAGTGGTTAGAGCTAGTTTGACAGGGGCGACAGTCTTTTCAACCATTCATGCCAAGAGTATCCGAGGAGTTTAT
GAACGCCTTCTGGAGTTGGGTGTGACGGAGGAGGAACTAGCAGTTGTCCTGCAAGGCGTCTGCTACCAGAGATTAATTGG
GGGAGGAGGAATCGTTGACTTTGCAAACAAAGACTTTCAAGAACACCAGCCAACTAGCTGGAATGAACAGATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

89.137

100

0.891
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

88.179

100

0.882
  comGA/cglA/cilD Streptococcus pneumoniae D39

88.179

100

0.882
  comGA/cglA/cilD Streptococcus pneumoniae R6

88.179

100

0.882
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

88.179

100

0.882
  comYA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.968

99.042

0.604
  comGA Lactococcus lactis subsp. cremoris KW2

55.449

99.681

0.553
  comGA Latilactobacillus sakei subsp. sakei 23K

43.726

84.026

0.367

Multiple sequence alignment