Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   NYE79_RS01040 Genome accession   NZ_CP150168
Coordinates   182515..183531 (+) Length   338 a.a.
NCBI ID   WP_216728921.1    Uniprot ID   -
Organism   Streptococcus sp. FSL W8-0197     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 177515..188531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYE79_RS01020 (NYE79_01020) - 177932..179749 (+) 1818 WP_216728917.1 acyltransferase family protein -
  NYE79_RS01025 (NYE79_01025) nagA 179890..181041 (+) 1152 WP_216728918.1 N-acetylglucosamine-6-phosphate deacetylase -
  NYE79_RS01030 (NYE79_01030) - 181196..181561 (+) 366 WP_216728919.1 DUF1033 family protein -
  NYE79_RS01035 (NYE79_01035) comGA/cglA/cilD 181626..182567 (+) 942 WP_216728920.1 competence type IV pilus ATPase ComGA Machinery gene
  NYE79_RS01040 (NYE79_01040) comGB/cglB 182515..183531 (+) 1017 WP_216728921.1 competence type IV pilus assembly protein ComGB Machinery gene
  NYE79_RS01045 (NYE79_01045) comGC/cglC 183533..183856 (+) 324 WP_000735800.1 competence type IV pilus major pilin ComGC Machinery gene
  NYE79_RS01050 (NYE79_01050) comGD/cglD 183819..184253 (+) 435 WP_216728922.1 competence type IV pilus minor pilin ComGD Machinery gene
  NYE79_RS01055 (NYE79_01055) comGE/cglE 184216..184518 (+) 303 WP_200370534.1 competence type IV pilus minor pilin ComGE Machinery gene
  NYE79_RS01060 (NYE79_01060) comGF/cglF 184481..184942 (+) 462 WP_070568120.1 competence type IV pilus minor pilin ComGF Machinery gene
  NYE79_RS01065 (NYE79_01065) comGG/cglG 184920..185333 (+) 414 WP_070568123.1 competence type IV pilus minor pilin ComGG Machinery gene
  NYE79_RS01070 (NYE79_01070) - 185366..185956 (+) 591 WP_216728923.1 class I SAM-dependent methyltransferase -
  NYE79_RS01075 (NYE79_01075) comYH 186015..186968 (+) 954 WP_216728924.1 class I SAM-dependent methyltransferase Machinery gene
  NYE79_RS01080 (NYE79_01080) - 187019..188209 (+) 1191 WP_000167790.1 acetate kinase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38449.59 Da        Isoelectric Point: 9.5636

>NTDB_id=964579 NYE79_RS01040 WP_216728921.1 182515..183531(+) (comGB/cglB) [Streptococcus sp. FSL W8-0197]
MDISQVFRLKRKKLATAKQKKIITLFNNLFSSGFHLVEIISFLGRSALLEKDYVAQMHQGLAQGKSFSEMMDSLGFSSAI
VTQLSLAEVHGNLHLSLGKIEEYLENLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLGSSNIATQIIGNLPQIF
LGLVLVCSLSLLLALTFYKRSSKMRVFSMLARIPFLGIFVQTYLTAYYAREWGNMISQGMELTQIFQIMQEQGSQLFKEI
GQDLAQALQNGHEFSQTIATYPFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEVFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=964579 NYE79_RS01040 WP_216728921.1 182515..183531(+) (comGB/cglB) [Streptococcus sp. FSL W8-0197]
ATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAAGCAGAAGAAAATCATCACTTTGTTTAA
CAACCTCTTCTCCAGTGGCTTTCATTTGGTGGAAATTATTTCTTTCTTGGGAAGAAGTGCCCTGCTAGAAAAGGACTATG
TGGCCCAGATGCACCAAGGCTTGGCTCAGGGGAAATCATTCTCAGAGATGATGGACAGCTTGGGCTTTTCAAGTGCTATT
GTGACCCAATTATCTCTAGCTGAAGTGCATGGAAATCTCCACCTGAGTCTGGGAAAAATAGAAGAATATCTGGAAAATCT
GGCCAAGGTCAAGAAGAAGTTAATCGAAGTGGCGACCTATCCCCTGATTTTGCTGGGATTTCTCCTGCTAATCATGCTGG
GGCTCAGGAATTATTTGCTCCCTCAACTGGGCAGTAGCAATATCGCCACTCAAATCATCGGCAATCTGCCACAAATTTTT
CTGGGGCTAGTGTTGGTTTGCTCTCTATCTTTACTTTTAGCCCTCACTTTCTACAAAAGAAGTTCCAAGATGCGGGTCTT
TTCGATGTTAGCGCGGATTCCCTTCCTAGGAATCTTTGTCCAGACCTATCTGACAGCCTATTACGCGCGTGAATGGGGCA
ATATGATTTCGCAGGGGATGGAGCTGACGCAGATTTTTCAGATCATGCAGGAACAAGGCTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAGCCCTGCAAAATGGCCACGAATTTTCTCAAACCATAGCGACCTATCCTTTCTTTAAAAAGGA
GTTGAGTCTCATCATCGAGTATGGAGAAGTCAAGTCCAAGCTGGGGAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGTCTTTTTTACCCGAGTTAATCGTACCATGAATCTGGTACAGCCACTGGTTTTTATCTTTGTGGCCCTGATTATCGTT
TTACTTTATGCGGCAATGCTTATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis SK321

92.604

100

0.926
  comGB/cglB Streptococcus mitis NCTC 12261

92.012

100

0.92
  comGB/cglB Streptococcus pneumoniae Rx1

89.941

100

0.899
  comGB/cglB Streptococcus pneumoniae D39

89.941

100

0.899
  comGB/cglB Streptococcus pneumoniae R6

89.941

100

0.899
  comGB/cglB Streptococcus pneumoniae TIGR4

89.941

100

0.899
  comYB Streptococcus gordonii str. Challis substr. CH1

73.81

99.408

0.734
  comYB Streptococcus mutans UA140

59.248

94.379

0.559
  comYB Streptococcus mutans UA159

59.248

94.379

0.559
  comGB Lactococcus lactis subsp. cremoris KW2

51.479

100

0.515

Multiple sequence alignment