Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WIR04_RS08110 Genome accession   NZ_CP149130
Coordinates   1746020..1747159 (-) Length   379 a.a.
NCBI ID   WP_338891805.1    Uniprot ID   -
Organism   Aeromonas rivipollensis strain BE1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1741020..1752159
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WIR04_RS08085 - 1741104..1741433 (-) 330 WP_338891796.1 translation initiation factor -
  WIR04_RS08090 - 1741554..1741781 (+) 228 WP_338891798.1 FeoA family protein -
  WIR04_RS08095 feoB 1741820..1744090 (+) 2271 WP_338891800.1 Fe(2+) transporter permease subunit FeoB -
  WIR04_RS08100 - 1744134..1744376 (+) 243 WP_106886441.1 FeoC-like transcriptional regulator -
  WIR04_RS08105 glgC 1744481..1745752 (-) 1272 WP_338891803.1 glucose-1-phosphate adenylyltransferase -
  WIR04_RS08110 pilU 1746020..1747159 (-) 1140 WP_338891805.1 type IVa pilus ATPase TapW Machinery gene
  WIR04_RS08115 - 1747215..1747571 (-) 357 WP_025327402.1 DMT family protein -
  WIR04_RS08120 - 1747684..1748433 (+) 750 WP_025327401.1 substrate-binding periplasmic protein -
  WIR04_RS08125 btuC 1748521..1749528 (+) 1008 WP_338891808.1 vitamin B12 ABC transporter permease BtuC -
  WIR04_RS08130 - 1749515..1750300 (+) 786 WP_338891810.1 ATP-binding cassette domain-containing protein -
  WIR04_RS08135 - 1750534..1751601 (-) 1068 WP_307764418.1 3-deoxy-7-phosphoheptulonate synthase -
  WIR04_RS08140 - 1751743..1752123 (+) 381 WP_005328065.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42327.32 Da        Isoelectric Point: 6.4282

>NTDB_id=956765 WIR04_RS08110 WP_338891805.1 1746020..1747159(-) (pilU) [Aeromonas rivipollensis strain BE1]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGALRPLDEVPLEPGEVARIAADLMDSEQKQQFEKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEVMLGTHTVRDLIKRGEFGGLKEIMEKSRALGMVTFDSTL
FDLVVEGVIDEEEAVKNADSQNNLRLKIKLWREKGQIGNSAAASGWSLEPTKDDNNDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=956765 WIR04_RS08110 WP_338891805.1 1746020..1747159(-) (pilU) [Aeromonas rivipollensis strain BE1]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCAGACCTCTATCTCTCCACAGGGGCGCCCCCCTG
CGCCAAGTTCAACGGCGCCCTGCGCCCCCTGGACGAGGTGCCGCTCGAGCCGGGGGAGGTAGCCAGGATAGCCGCCGATC
TCATGGACTCGGAGCAGAAGCAGCAGTTCGAGAAGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGGCGC
TTTCGGGTCAACATCTTCAAGCAGCGCAACGAGGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTCAAACTCCCCCCTGTGCTGCTCGACACCATCATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGCACCGGCT
CTGGCAAGTCCACGTCGCTCGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGTGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGACACCCGCAGCTTCCACGC
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTCATCCTCATCGGTGAAATTCGCGACCGGGAGACCATGGAGCATG
CGCTGGCCTTCTCCGAGACCGGGCATCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCCGAGGAGCGCAGGCCCCAGCTGCTCAACGATCTGGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
GCTGGTGAAGACCACGGACGGCGGGCGCCGGGCGGCGGTGGAGGTGATGCTCGGCACCCACACTGTGCGCGATCTCATCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGATCATGGAGAAATCCAGGGCCCTTGGCATGGTCACCTTCGACAGCACCCTG
TTCGATCTGGTGGTGGAGGGGGTGATAGACGAAGAAGAGGCGGTCAAGAACGCAGACTCCCAGAACAACCTGCGCCTCAA
GATCAAGCTGTGGCGGGAGAAGGGGCAGATAGGCAACAGTGCGGCGGCCAGCGGCTGGAGCCTGGAGCCCACCAAGGATG
ATAACAACGATCTCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.4

98.945

0.538
  pilU Acinetobacter baylyi ADP1

52.676

93.668

0.493
  pilU Vibrio cholerae strain A1552

49.858

93.14

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

89.446

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.171

92.612

0.372
  pilT Vibrio cholerae strain A1552

40.171

92.612

0.372
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Legionella pneumophila strain ERS1305867

41.916

88.127

0.369
  pilT Legionella pneumophila strain Lp02

41.916

88.127

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361