Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WJL51_RS06880 Genome accession   NZ_CP149124
Coordinates   1573368..1574507 (+) Length   379 a.a.
NCBI ID   WP_139392833.1    Uniprot ID   A0AAD3YMJ9
Organism   Aeromonas hydrophila strain BE2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1568368..1579507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJL51_RS06850 - 1568675..1569739 (+) 1065 WP_045789462.1 3-deoxy-7-phosphoheptulonate synthase -
  WJL51_RS06855 aac(6') 1569746..1570204 (+) 459 WP_202004133.1 aminoglycoside 6'-N-acetyltransferase -
  WJL51_RS06860 - 1570226..1571011 (-) 786 WP_339331109.1 ATP-binding cassette domain-containing protein -
  WJL51_RS06865 btuC 1570998..1572005 (-) 1008 WP_339331110.1 vitamin B12 ABC transporter permease BtuC -
  WJL51_RS06870 - 1572106..1572837 (-) 732 WP_029303898.1 transporter substrate-binding domain-containing protein -
  WJL51_RS06875 - 1572951..1573307 (+) 357 WP_005302630.1 DMT family protein -
  WJL51_RS06880 pilU 1573368..1574507 (+) 1140 WP_139392833.1 type IVa pilus ATPase TapW Machinery gene
  WJL51_RS06885 glgC 1574790..1576061 (+) 1272 WP_011706551.1 glucose-1-phosphate adenylyltransferase -
  WJL51_RS06890 - 1576197..1576676 (+) 480 WP_339331111.1 WbuC family cupin fold metalloprotein -
  WJL51_RS06895 - 1576786..1577028 (-) 243 WP_011706553.1 FeoC-like transcriptional regulator -
  WJL51_RS06900 feoB 1577025..1579298 (-) 2274 WP_339331112.1 Fe(2+) transporter permease subunit FeoB -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42278.35 Da        Isoelectric Point: 6.4290

>NTDB_id=956651 WJL51_RS06880 WP_139392833.1 1573368..1574507(+) (pilU) [Aeromonas hydrophila strain BE2]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVARIADAIMDGEQKQQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=956651 WJL51_RS06880 WP_139392833.1 1573368..1574507(+) (pilU) [Aeromonas hydrophila strain BE2]
ATGGAATTGCGAGACATGCTACAGATCCTGGCTAAGCAGGATGGCTCCGACCTCTATCTGTCGACCGGCGCCCCCCCTTG
CGCCAAGTTCAACGGCGGCCTACGTCCGCTCAGTGAGACGCCGCTCGAACCCGGCGAGGTGGCCAGAATAGCCGACGCCA
TCATGGACGGCGAGCAAAAGCAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCGCTGCCCCAGATCGGCCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTGTCGCTGGTGGCCCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTGAAACTGCCCCCCGTGCTGCTCGACACCATCATGGAGAAGCGTGGCCTGGTGCTGTTCGTCGGCGGTACCGGCT
CGGGCAAGTCCACCTCGCTCGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTGCACCGTCACCGCAAGAGCATCATCAACCAGCGCGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
CGCCCTCAAGAACACCCTGCGCCAGGCGCCCGATGTCATCCTTATCGGCGAGATCCGCGATCGCGAGACCATGGAGCATG
CGCTGGCCTTCTCCGAGACCGGCCACCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCCCAGCTGCTCAACGACCTTGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTCAAAACCACGGACGGTGGCCGCCGGGCAGCGGTGGAGATCATGCTGGGTACCCACACCATCCGCGACATGATCA
AACGCGGCGAATTTGGCGGCCTCAAGGAGGTGATGGAGAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTAGTCGAGGGTGTGATCGACGAGGAGGAGGCGGTGAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATCGCGAGCAGCAGCGATGCCACTGGCTGGAGCCTCGAGCCCACCAAGGACG
AAAAAGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.133

98.945

0.536
  pilU Acinetobacter baylyi ADP1

53.521

93.668

0.501
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.076

93.14

0.383
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Legionella pneumophila strain ERS1305867

41.916

88.127

0.369
  pilT Legionella pneumophila strain Lp02

41.916

88.127

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361