Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   V6090_RS06325 Genome accession   NZ_CP145798
Coordinates   1369297..1370436 (-) Length   379 a.a.
NCBI ID   WP_005347150.1    Uniprot ID   K1IT58
Organism   Aeromonas veronii strain CARB25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1364297..1375436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6090_RS06300 (V6090_06305) oppF 1364878..1365873 (+) 996 WP_064339830.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -
  V6090_RS06305 (V6090_06310) - 1366176..1367120 (+) 945 WP_005337664.1 TerC family protein -
  V6090_RS06310 (V6090_06315) - 1367117..1367251 (+) 135 WP_272936816.1 hypothetical protein -
  V6090_RS06315 (V6090_06320) alr 1367317..1368543 (-) 1227 WP_227096226.1 alanine racemase -
  V6090_RS06320 (V6090_06325) - 1368717..1369166 (+) 450 WP_064339832.1 isoprenylcysteine carboxylmethyltransferase family protein -
  V6090_RS06325 (V6090_06330) pilU 1369297..1370436 (-) 1140 WP_005347150.1 type IVa pilus ATPase TapW Machinery gene
  V6090_RS06330 (V6090_06335) - 1370495..1370851 (-) 357 WP_005353120.1 DMT family protein -
  V6090_RS06335 (V6090_06340) - 1370964..1371695 (+) 732 WP_013723600.1 ABC transporter substrate-binding protein -
  V6090_RS06340 (V6090_06345) btuC 1371796..1372803 (+) 1008 WP_043825636.1 vitamin B12 ABC transporter permease BtuC -
  V6090_RS06345 (V6090_06350) - 1372869..1373666 (+) 798 WP_064339833.1 ATP-binding cassette domain-containing protein -
  V6090_RS06350 (V6090_06355) - 1373783..1374844 (-) 1062 WP_005347142.1 3-deoxy-7-phosphoheptulonate synthase -
  V6090_RS06355 (V6090_06360) - 1374989..1375369 (+) 381 WP_005337641.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42311.38 Da        Isoelectric Point: 6.4293

>NTDB_id=939883 V6090_RS06325 WP_005347150.1 1369297..1370436(-) (pilU) [Aeromonas veronii strain CARB25]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLNETPLEPGEVARIAAEIMDGEQKQQFEKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDFF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=939883 V6090_RS06325 WP_005347150.1 1369297..1370436(-) (pilU) [Aeromonas veronii strain CARB25]
ATGGAATTGCGAGACATGTTACAGATCCTGGCCAAGCAGGATGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
CGCCAAATTTAACGGCGGCCTGCGCCCCCTCAACGAAACGCCGCTGGAACCGGGTGAAGTGGCGCGGATCGCCGCCGAGA
TCATGGATGGCGAGCAGAAGCAGCAGTTCGAGAAGGAGTTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATCGGCCGC
TTTCGGGTCAACATCTTCAAGCAGCGCAACGAAGTCTCTCTGGTGGCCCGCAACATCAAGACCGAGATCCCCCGTTTTGA
AGATCTCAAACTCCCGCCTGTGCTGCTCGACACCATCATGGAGAAACGTGGCCTGGTGCTGTTTGTCGGCGGTACCGGCT
CGGGCAAGTCCACCTCGCTCGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTTCATCGCCATCGCAAGAGCATAATCAACCAGCGGGAGGTTGGCGTGGATACCCGCAGCTTCCATGC
GGCCCTGAAAAACACCCTGCGTCAGGCGCCGGACGTCATTCTCATCGGCGAGATCCGTGATCGCGAGACCATGGAACATG
CGCTGGCCTTCTCCGAAACCGGCCACCTTGCCATCTCCACCCTTCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCCGAGGAACGCCGCCCCCAGCTGCTCAACGACCTTGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
GCTGGTGAAAACCACCGATGGCGGCCGCCGAGCCGCGGTGGAGATCATGCTGGGCACCCACACCATCCGCGACATGATCA
AGCGCGGCGAGTTTGGCGGCCTCAAAGAGGTGATGGAAAAATCCAAAGCCCTCGGCATGGTCACCTTCGACAGTGCCCTG
TTTGATCTGGTGGTGGAAGGCGTGATCGACGAGGAGGAGGCAGTGAAGAACGCCGATTCGGCCAACAACCTGCGTCTCAA
GATCAAACTGTGGAAGGAGAAGGGGCAGATCGCCAGCAGCAGTGATGCCACCGGCTGGAGTCTCGAACCCACCAAGGACG
AGAAAGACGATTTTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K1IT58

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.211

100

0.544
  pilU Acinetobacter baylyi ADP1

53.239

93.668

0.499
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.36

93.14

0.385
  pilT Legionella pneumophila strain ERS1305867

42.515

88.127

0.375
  pilT Legionella pneumophila strain Lp02

42.515

88.127

0.375
  pilT Pseudomonas aeruginosa PAK

41.279

90.765

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.698

90.765

0.369
  pilT Vibrio cholerae strain A1552

42.042

87.863

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.042

87.863

0.369
  pilT Acinetobacter baumannii D1279779

41.617

88.127

0.367
  pilT Acinetobacter baumannii strain A118

41.617

88.127

0.367
  pilT Acinetobacter nosocomialis M2

41.617

88.127

0.367