Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   V6Z53_RS28720 Genome accession   NZ_CP145732
Coordinates   6269668..6270087 (-) Length   139 a.a.
NCBI ID   WP_338583097.1    Uniprot ID   -
Organism   Pseudomonas sp. MAG733B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6264668..6275087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6Z53_RS28705 (V6Z53_28705) - 6265485..6268436 (+) 2952 WP_338583092.1 DUF748 domain-containing protein -
  V6Z53_RS28710 (V6Z53_28710) - 6268436..6268732 (+) 297 WP_338586586.1 DUF2845 domain-containing protein -
  V6Z53_RS28715 (V6Z53_28715) - 6269002..6269358 (-) 357 WP_338583094.1 BON domain-containing protein -
  V6Z53_RS28720 (V6Z53_28720) pilA 6269668..6270087 (-) 420 WP_338583097.1 pilin Machinery gene
  V6Z53_RS28725 (V6Z53_28725) pilB 6270313..6272013 (+) 1701 WP_338583098.1 type IV-A pilus assembly ATPase PilB Machinery gene
  V6Z53_RS28730 (V6Z53_28730) pilC 6272016..6273233 (+) 1218 WP_338583099.1 type II secretion system F family protein Machinery gene
  V6Z53_RS28735 (V6Z53_28735) pilD 6273235..6274107 (+) 873 WP_338583101.1 A24 family peptidase Machinery gene
  V6Z53_RS28740 (V6Z53_28740) coaE 6274104..6274727 (+) 624 WP_338583103.1 dephospho-CoA kinase -
  V6Z53_RS28745 (V6Z53_28745) yacG 6274724..6274924 (+) 201 WP_060543361.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14176.34 Da        Isoelectric Point: 8.1062

>NTDB_id=939866 V6Z53_RS28720 WP_338583097.1 6269668..6270087(-) (pilA) [Pseudomonas sp. MAG733B]
MNTQKGFSLIELLIVVAIIGILATFAIPAYSKYQARAKVTAGLAEISALKVPFEDIINQGTDPDLTKVGGTAATSNCTTT
ASGKAADGTGTIGCTILNAPGPVLNKTITLSRTVTGWTCATTVDQEYAPKGCTGVAPKA

Nucleotide


Download         Length: 420 bp        

>NTDB_id=939866 V6Z53_RS28720 WP_338583097.1 6269668..6270087(-) (pilA) [Pseudomonas sp. MAG733B]
ATGAACACTCAAAAAGGTTTTTCCCTGATCGAGCTGCTGATCGTGGTGGCGATCATCGGGATTCTCGCGACGTTCGCGAT
ACCGGCTTACTCCAAGTATCAGGCGCGGGCCAAGGTGACGGCGGGGCTGGCCGAGATTTCGGCGTTGAAGGTGCCGTTCG
AGGACATTATCAATCAAGGGACCGACCCTGATTTGACCAAGGTTGGCGGCACAGCAGCCACCTCGAACTGCACCACCACG
GCCTCGGGTAAAGCCGCTGATGGCACCGGGACTATTGGTTGCACGATCCTTAATGCGCCGGGTCCGGTACTGAATAAAAC
CATTACCCTTTCGCGTACGGTGACTGGCTGGACGTGTGCCACAACTGTGGATCAGGAATACGCGCCGAAGGGCTGCACGG
GGGTTGCTCCGAAAGCTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

48.592

100

0.496
  pilA/pilAI Pseudomonas stutzeri DSM 10701

48.905

98.561

0.482
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

45.39

100

0.46
  pilA Vibrio cholerae strain A1552

45.39

100

0.46
  pilA Vibrio cholerae C6706

45.39

100

0.46
  pilA Pseudomonas aeruginosa PAK

41.611

100

0.446
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.185

97.122

0.439
  pilA2 Legionella pneumophila str. Paris

43.704

97.122

0.424
  pilA2 Legionella pneumophila strain ERS1305867

41.844

100

0.424
  comP Acinetobacter baylyi ADP1

38.095

100

0.403