Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   V6Z53_RS28725 Genome accession   NZ_CP145732
Coordinates   6270313..6272013 (+) Length   566 a.a.
NCBI ID   WP_338583098.1    Uniprot ID   -
Organism   Pseudomonas sp. MAG733B     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6265313..6277013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6Z53_RS28705 (V6Z53_28705) - 6265485..6268436 (+) 2952 WP_338583092.1 DUF748 domain-containing protein -
  V6Z53_RS28710 (V6Z53_28710) - 6268436..6268732 (+) 297 WP_338586586.1 DUF2845 domain-containing protein -
  V6Z53_RS28715 (V6Z53_28715) - 6269002..6269358 (-) 357 WP_338583094.1 BON domain-containing protein -
  V6Z53_RS28720 (V6Z53_28720) pilA 6269668..6270087 (-) 420 WP_338583097.1 pilin Machinery gene
  V6Z53_RS28725 (V6Z53_28725) pilB 6270313..6272013 (+) 1701 WP_338583098.1 type IV-A pilus assembly ATPase PilB Machinery gene
  V6Z53_RS28730 (V6Z53_28730) pilC 6272016..6273233 (+) 1218 WP_338583099.1 type II secretion system F family protein Machinery gene
  V6Z53_RS28735 (V6Z53_28735) pilD 6273235..6274107 (+) 873 WP_338583101.1 A24 family peptidase Machinery gene
  V6Z53_RS28740 (V6Z53_28740) coaE 6274104..6274727 (+) 624 WP_338583103.1 dephospho-CoA kinase -
  V6Z53_RS28745 (V6Z53_28745) yacG 6274724..6274924 (+) 201 WP_060543361.1 DNA gyrase inhibitor YacG -
  V6Z53_RS28750 (V6Z53_28750) - 6274960..6275649 (-) 690 WP_338583107.1 energy-coupling factor ABC transporter permease -
  V6Z53_RS28755 (V6Z53_28755) - 6275837..6276079 (+) 243 WP_338583108.1 DUF2790 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62855.00 Da        Isoelectric Point: 5.7003

>NTDB_id=939867 V6Z53_RS28725 WP_338583098.1 6270313..6272013(+) (pilB) [Pseudomonas sp. MAG733B]
MNDIALSGLAKQLVLAELLTDKSAQQASQQAQRNRISLVSYLVQNKLVKSRQVAEIASEHFGMALLDLHALDKEIQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFDTHGTGLEDMADVDLEG
LDIETIDDKRQDAIVGQDADDAPVVRFVHKMLLDAIRSGSSDLHFEPYEKIYRVRMRTDGMLREVAKPPIQLANRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQQELYMAALRQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGMDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKASQTGHLVLSTLHTNSAAQTLTRLQNMGIQGFNIATSVSLIIAQRLARKLCSHCRVPLDIPREALLKEGFPE
ERIGTFTIYEPVGCEQCNGGYKGRVGIYEVVKNTADLQRLIMAEGNSLDIDEQMRKDGFNDLRTSGLLKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=939867 V6Z53_RS28725 WP_338583098.1 6270313..6272013(+) (pilB) [Pseudomonas sp. MAG733B]
ATGAATGACATCGCCCTCAGCGGCCTGGCCAAGCAATTGGTCCTGGCCGAGCTGCTCACTGACAAAAGCGCGCAACAGGC
GTCCCAGCAAGCCCAACGCAACCGCATCTCCCTGGTCAGCTACCTGGTTCAGAACAAACTGGTCAAAAGCCGCCAGGTCG
CCGAGATCGCCTCGGAGCATTTCGGCATGGCCTTGCTGGACCTTCATGCCCTGGACAAGGAGATTCAGCCCAAGGGCCTG
GTCAGTGAGAAGCTGGTCCGCCAGCACCACGCGCTGCCCCTCTGGCGCCGAGGCAACAAACTGTTCGTGGGGATTTCCGA
CCCGACCAATCACCAGGCCATCAACGACATCCAGTTCAGTACCGGGCTTACGACCGAAGCCATCCTCGTGGAGGACGACA
AGCTCAGCGACGCCATCGAGAAGTTCTTCGACACCCATGGGACCGGCCTGGAAGACATGGCCGATGTCGACCTCGAAGGC
CTGGACATCGAGACCATCGATGACAAGCGTCAGGACGCCATCGTCGGCCAGGATGCCGATGACGCGCCGGTGGTGCGCTT
CGTCCACAAAATGCTGCTCGACGCGATCAGGAGCGGTTCTTCCGACCTGCATTTCGAGCCCTACGAGAAAATCTATCGCG
TGCGGATGCGCACCGACGGCATGCTGCGGGAAGTCGCCAAGCCGCCGATCCAATTGGCCAATCGAATCGCCGCGCGGCTG
AAGGTCATGGCCAGCCTCGACATCTCCGAACGCCGCAAGCCCCAGGACGGGCGGATCAAGATGCGCCTGTCCAAGAGCAA
ATCCATCGACTTTCGGGTCAACACCTTGCCGACCCTGTGGGGCGAGAAAGTGGTGATCCGGATTCTTGATCCCTCCAGCG
CGCAAATGGGCATCGACGCCCTCGGCTACGAGCCTGAACAACAAGAACTGTACATGGCCGCACTCAGGCAACCTCAAGGC
ATGATTCTGGTGACCGGCCCCACCGGCTCGGGCAAAACCGTTTCGCTCTACACCGGGCTGAACATCCTCAACACCGTGGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCGAAGCAAG
GGATGGACTTCGCCCAGGCGCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAAATCCGTGATCTG
GAAACCGCCGAAATCGCCATCAAGGCTTCGCAGACCGGGCACCTGGTGCTGTCGACCCTGCACACCAACAGTGCCGCACA
AACCCTGACCCGCTTGCAGAACATGGGCATCCAGGGTTTCAACATCGCCACGTCCGTCAGCCTGATCATCGCCCAGCGCC
TGGCGCGCAAACTGTGCAGCCATTGCCGGGTGCCGCTCGACATTCCCCGCGAGGCGCTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCACATTCACGATCTATGAGCCGGTCGGCTGCGAACAATGCAACGGCGGCTACAAAGGGCGAGTGGGGAT
TTATGAAGTGGTGAAGAACACAGCGGACTTGCAACGATTGATCATGGCCGAAGGCAACTCACTGGACATCGACGAGCAGA
TGCGCAAGGACGGCTTCAACGACCTGCGCACCTCGGGCCTGCTCAAAGCCATGCAGGGCATTACCAGCCTTGAAGAAATC
AACCGGGTTACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.23

99.823

0.581
  pilB Acinetobacter baumannii D1279779

57.27

99.647

0.571
  pilB Legionella pneumophila strain ERS1305867

55.83

100

0.558
  pilB Vibrio cholerae strain A1552

50.265

100

0.504
  pilB Vibrio campbellii strain DS40M4

49.123

100

0.495
  pilF Neisseria gonorrhoeae MS11

48.845

99.47

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

49.281

98.233

0.484
  pilF Thermus thermophilus HB27

36.283

99.823

0.362
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.563

93.463

0.36