Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Aeroheme_RS07990 Genome accession   NZ_CP144265
Coordinates   1714250..1715389 (-) Length   379 a.a.
NCBI ID   WP_106886444.1    Uniprot ID   A0AAW9YFE9
Organism   Aeromonas sp. DSM 116730     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1709250..1720389
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Aeroheme_RS07965 (Aeroheme_01589) - 1709334..1709663 (-) 330 WP_163149208.1 translation initiation factor -
  Aeroheme_RS07970 (Aeroheme_01590) - 1709783..1710010 (+) 228 WP_025327407.1 FeoA family protein -
  Aeroheme_RS07975 (Aeroheme_01591) feoB 1710007..1712277 (+) 2271 WP_411167036.1 Fe(2+) transporter permease subunit FeoB -
  Aeroheme_RS07980 (Aeroheme_01592) - 1712321..1712563 (+) 243 WP_163153777.1 FeoC-like transcriptional regulator -
  Aeroheme_RS07985 (Aeroheme_01593) glgC 1712667..1713938 (-) 1272 WP_111912664.1 glucose-1-phosphate adenylyltransferase -
  Aeroheme_RS07990 (Aeroheme_01594) pilU 1714250..1715389 (-) 1140 WP_106886444.1 type IVa pilus ATPase TapW Machinery gene
  Aeroheme_RS07995 (Aeroheme_01595) - 1715445..1715801 (-) 357 WP_010675200.1 DMT family protein -
  Aeroheme_RS08000 (Aeroheme_01596) - 1715914..1716663 (+) 750 WP_168235349.1 substrate-binding periplasmic protein -
  Aeroheme_RS08005 (Aeroheme_01597) btuC 1716750..1717757 (+) 1008 WP_139745173.1 vitamin B12 ABC transporter permease BtuC -
  Aeroheme_RS08010 (Aeroheme_01598) - 1717744..1718529 (+) 786 WP_411167037.1 ATP-binding cassette domain-containing protein -
  Aeroheme_RS08015 (Aeroheme_01599) aac(6') 1718552..1719010 (-) 459 WP_408779299.1 aminoglycoside 6'-N-acetyltransferase -
  Aeroheme_RS08020 (Aeroheme_01600) - 1719017..1720081 (-) 1065 WP_408779300.1 3-deoxy-7-phosphoheptulonate synthase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42354.39 Da        Isoelectric Point: 6.6006

>NTDB_id=933072 Aeroheme_RS07990 WP_106886444.1 1714250..1715389(-) (pilU) [Aeromonas sp. DSM 116730]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGALRPLNEVPLEPGEVARIAAELMDSEQKQQFEKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEVMLGTHTIRDLIKRGEFGGLKEIMEKSRALGMVTFDSTL
FDLVVEGVIDEEEAVKNADSQNNLRLKIKLWREKGQIGNSAAASGWSLEPTKDDNNDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=933072 Aeroheme_RS07990 WP_106886444.1 1714250..1715389(-) (pilU) [Aeromonas sp. DSM 116730]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCCGACCTCTATCTCTCCACAGGGGCGCCCCCCTG
CGCCAAGTTCAACGGCGCCCTGCGCCCCCTGAACGAGGTGCCGCTCGAGCCGGGCGAGGTGGCCAGGATAGCCGCCGAGC
TCATGGACTCGGAGCAGAAGCAGCAGTTCGAGAAGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGCCGC
TTTCGGGTCAACATCTTCAAGCAGCGCAACGAGGTCTCCCTGGTGGCGCGCAACATCAAGACAGAGATCCCCCGCTTCGA
GGACTTGAAGCTTCCCCCGGTGCTGCTCGACACCATCATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGCACCGGCT
CGGGCAAGTCCACCTCGCTCGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTCATCCTCATAGGTGAAATTCGCGACCGGGAGACCATGGAGCATG
CGCTGGCCTTCTCCGAGACCGGGCATCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCCGAGGAGCGCAGGCCCCAGCTGCTCAACGATCTTGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
ACTGGTGAAGACCACAGACGGCGGCCGCCGGGCGGCGGTGGAGGTCATGCTCGGCACCCACACCATTCGCGATCTCATCA
AACGCGGCGAGTTTGGCGGCCTCAAGGAGATCATGGAGAAATCCAGGGCCCTTGGCATGGTCACCTTCGACAGCACCCTG
TTCGATCTGGTGGTGGAGGGGGTGATAGACGAAGAAGAGGCGGTCAAGAACGCAGATTCCCAGAACAACCTGCGCCTCAA
GATCAAGCTGTGGAGAGAGAAGGGGCAGATAGGCAACAGTGCGGCGGCCAGCGGCTGGAGCCTGGAACCCACCAAGGATG
ATAACAACGATCTCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.667

98.945

0.541
  pilU Acinetobacter baylyi ADP1

52.676

93.668

0.493
  pilU Vibrio cholerae strain A1552

50.142

93.14

0.467
  pilT Vibrio cholerae strain A1552

40.741

92.612

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.741

92.612

0.377
  pilT Legionella pneumophila strain ERS1305867

42.216

88.127

0.372
  pilT Legionella pneumophila strain Lp02

42.216

88.127

0.372
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.593

89.446

0.372
  pilT Pseudomonas aeruginosa PAK

40.988

90.765

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.407

90.765

0.367
  pilT Acinetobacter baumannii strain A118

41.317

88.127

0.364
  pilT Acinetobacter nosocomialis M2

41.317

88.127

0.364
  pilT Acinetobacter baumannii D1279779

41.317

88.127

0.364