Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   U3H55_RS07750 Genome accession   NZ_CP140792
Coordinates   1647643..1648782 (-) Length   379 a.a.
NCBI ID   WP_322863867.1    Uniprot ID   -
Organism   Aeromonas allosaccharophila strain M01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1642643..1653782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U3H55_RS07725 oppF 1643127..1644122 (+) 996 WP_042057772.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -
  U3H55_RS07730 - 1644425..1645369 (+) 945 WP_033137996.1 TerC family protein -
  U3H55_RS07735 - 1645497..1645631 (+) 135 WP_290439296.1 hypothetical protein -
  U3H55_RS07740 alr 1645697..1646923 (-) 1227 WP_322863865.1 alanine racemase -
  U3H55_RS07745 - 1647097..1647546 (+) 450 WP_322863866.1 isoprenylcysteine carboxylmethyltransferase family protein -
  U3H55_RS07750 pilU 1647643..1648782 (-) 1140 WP_322863867.1 type IVa pilus ATPase TapW Machinery gene
  U3H55_RS07755 - 1648841..1649197 (-) 357 WP_005353120.1 DMT family protein -
  U3H55_RS07760 - 1649311..1650042 (+) 732 WP_322863868.1 transporter substrate-binding domain-containing protein -
  U3H55_RS07765 btuC 1650142..1651149 (+) 1008 WP_322863869.1 vitamin B12 ABC transporter permease BtuC -
  U3H55_RS07770 - 1651136..1651933 (+) 798 WP_322863870.1 ATP-binding cassette domain-containing protein -
  U3H55_RS07775 - 1652091..1653158 (-) 1068 WP_322863871.1 3-deoxy-7-phosphoheptulonate synthase -
  U3H55_RS07780 - 1653303..1653683 (+) 381 WP_005337641.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42308.41 Da        Isoelectric Point: 6.5128

>NTDB_id=915601 U3H55_RS07750 WP_322863867.1 1647643..1648782(-) (pilU) [Aeromonas allosaccharophila strain M01]
MELRDMLQILAQQDGSDLYLSTGAPPCAKFHGGMRPLNDTPLAPGDVARIAAEIMDGEQKQQFEKELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGSLKEVMEKSKALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDMF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=915601 U3H55_RS07750 WP_322863867.1 1647643..1648782(-) (pilU) [Aeromonas allosaccharophila strain M01]
ATGGAATTGCGAGACATGCTACAGATCCTTGCCCAGCAAGATGGCTCGGACCTTTATCTCTCAACCGGAGCCCCTCCCTG
CGCCAAATTCCACGGCGGCATGCGGCCTCTGAACGACACGCCACTGGCGCCCGGCGACGTGGCCAGGATCGCCGCCGAGA
TCATGGATGGCGAGCAGAAACAACAGTTCGAGAAGGAGCTGGAGATGAACCTTGCCATCTCCCTGCCCCAGATCGGCCGT
TTCCGGATCAACATCTTCAAGCAGCGTAACGAAGTCTCCCTGGTGGCCCGTAATATCAAGACCGAGATCCCCCGCTTTGA
AGATCTCAAACTGCCACCCGTGTTGCTCGATACCATCATGGAGAAGCGGGGACTGGTGCTGTTTGTCGGCGGCACCGGCT
CGGGCAAGTCCACCTCGCTGGCCGCCCTTATCGATCACCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAAGAT
CCGGTGGAGTTCGTGCATCGTCACCGCAAGAGCATCATCAACCAGCGGGAAGTTGGTGTGGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGTCAGGCGCCGGACGTCATCCTTATCGGCGAGATCCGCGATCGCGAGACCATGGAGCACG
CGCTGGCCTTCTCCGAAACCGGCCACCTTGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGTCCGCAACTGCTCAACGATCTGGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
GCTGGTGAAAACCACCGATGGCGGCCGCCGGGCGGCGGTGGAGATCATGCTGGGCACCCATACCATCCGCGACATGATCA
AACGTGGTGAATTTGGCAGCCTCAAAGAGGTGATGGAAAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATTGAGGAGGAAGAAGCAGTGAAGAACGCTGATTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGGCAAATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAACCCACCAAGGACG
AGAAGGACGATATGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.684

100

0.538
  pilU Acinetobacter baylyi ADP1

52.394

93.668

0.491
  pilU Vibrio cholerae strain A1552

49.714

92.348

0.459
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

89.446

0.375
  pilT Legionella pneumophila strain ERS1305867

41.617

88.127

0.367
  pilT Legionella pneumophila strain Lp02

41.617

88.127

0.367
  pilT Pseudomonas aeruginosa PAK

40.407

90.765

0.367
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361