Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   U0449_RS06170 Genome accession   NZ_CP139862
Coordinates   1228728..1229669 (+) Length   313 a.a.
NCBI ID   WP_000249567.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 05H0020-2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1223728..1234669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0449_RS06150 - 1223794..1225611 (+) 1818 WP_001220843.1 acyltransferase family protein -
  U0449_RS06155 nagA 1225764..1226915 (+) 1152 WP_050087279.1 N-acetylglucosamine-6-phosphate deacetylase -
  U0449_RS06160 - 1227078..1228136 (+) 1059 WP_000649473.1 zinc-dependent alcohol dehydrogenase family protein -
  U0449_RS06165 - 1228287..1228652 (+) 366 WP_000286415.1 DUF1033 family protein -
  U0449_RS06170 comGA/cglA/cilD 1228728..1229669 (+) 942 WP_000249567.1 competence type IV pilus ATPase ComGA Machinery gene
  U0449_RS06175 comGB/cglB 1229617..1230633 (+) 1017 WP_078136505.1 competence type IV pilus assembly protein ComGB Machinery gene
  U0449_RS06180 comGC/cglC 1230635..1230961 (+) 327 WP_000738630.1 comG operon protein ComGC Machinery gene
  U0449_RS06185 comGD/cglD 1230954..1231358 (+) 405 WP_322398090.1 competence type IV pilus minor pilin ComGD Machinery gene
  U0449_RS06190 comGE/cglE 1231321..1231623 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  U0449_RS06195 comGF/cglF 1231586..1232047 (+) 462 WP_000250540.1 competence type IV pilus minor pilin ComGF Machinery gene
  U0449_RS06200 comGG/cglG 1232025..1232438 (+) 414 WP_000265620.1 competence type IV pilus minor pilin ComGG Machinery gene
  U0449_RS06205 - 1232575..1233169 (+) 595 Protein_1212 class I SAM-dependent methyltransferase -
  U0449_RS06210 comYH 1233230..1234183 (+) 954 WP_050219032.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35573.48 Da        Isoelectric Point: 6.0083

>NTDB_id=911492 U0449_RS06170 WP_000249567.1 1228728..1229669(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 05H0020-2]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=911492 U0449_RS06170 WP_000249567.1 1228728..1229669(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 05H0020-2]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATCTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACCTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTTTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.361

100

0.994
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.486

100

0.965
  comYA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

42.642

84.665

0.361