Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   U0449_RS06175 Genome accession   NZ_CP139862
Coordinates   1229617..1230633 (+) Length   338 a.a.
NCBI ID   WP_078136505.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 05H0020-2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1224617..1235633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0449_RS06155 nagA 1225764..1226915 (+) 1152 WP_050087279.1 N-acetylglucosamine-6-phosphate deacetylase -
  U0449_RS06160 - 1227078..1228136 (+) 1059 WP_000649473.1 zinc-dependent alcohol dehydrogenase family protein -
  U0449_RS06165 - 1228287..1228652 (+) 366 WP_000286415.1 DUF1033 family protein -
  U0449_RS06170 comGA/cglA/cilD 1228728..1229669 (+) 942 WP_000249567.1 competence type IV pilus ATPase ComGA Machinery gene
  U0449_RS06175 comGB/cglB 1229617..1230633 (+) 1017 WP_078136505.1 competence type IV pilus assembly protein ComGB Machinery gene
  U0449_RS06180 comGC/cglC 1230635..1230961 (+) 327 WP_000738630.1 comG operon protein ComGC Machinery gene
  U0449_RS06185 comGD/cglD 1230954..1231358 (+) 405 WP_322398090.1 competence type IV pilus minor pilin ComGD Machinery gene
  U0449_RS06190 comGE/cglE 1231321..1231623 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  U0449_RS06195 comGF/cglF 1231586..1232047 (+) 462 WP_000250540.1 competence type IV pilus minor pilin ComGF Machinery gene
  U0449_RS06200 comGG/cglG 1232025..1232438 (+) 414 WP_000265620.1 competence type IV pilus minor pilin ComGG Machinery gene
  U0449_RS06205 - 1232575..1233169 (+) 595 Protein_1212 class I SAM-dependent methyltransferase -
  U0449_RS06210 comYH 1233230..1234183 (+) 954 WP_050219032.1 class I SAM-dependent methyltransferase Machinery gene
  U0449_RS06215 - 1234234..1235424 (+) 1191 WP_000167753.1 acetate kinase -
  U0449_RS06220 - 1235425..1235556 (+) 132 WP_000768904.1 hypothetical protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38494.51 Da        Isoelectric Point: 9.6073

>NTDB_id=911493 U0449_RS06175 WP_078136505.1 1229617..1230633(+) (comGB/cglB) [Streptococcus pneumoniae strain 05H0020-2]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMESLGFSSTI
ITQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=911493 U0449_RS06175 WP_078136505.1 1229617..1230633(+) (comGB/cglB) [Streptococcus pneumoniae strain 05H0020-2]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATTTTCAAGCACCATT
ATCACTCAGTTATCCCTAGCTGAAGTTCATGGAAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCTAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae D39

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae R6

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae TIGR4

98.817

100

0.988
  comGB/cglB Streptococcus mitis SK321

95.266

100

0.953
  comGB/cglB Streptococcus mitis NCTC 12261

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

71.131

99.408

0.707
  comYB Streptococcus mutans UA140

57.862

94.083

0.544
  comYB Streptococcus mutans UA159

57.862

94.083

0.544
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503