NTDB

Overview

Name	comGF/cglF	Type	Machinery gene
Locus tag	U0449_RS06195	Genome accession	NZ_CP139862
Coordinates	1231586..1232047 (+)	Length	153 a.a.
NCBI ID	WP_000250540.1	Uniprot ID	-
Organism	Streptococcus pneumoniae strain 05H0020-2
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1226586..1237047

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U0449_RS06160	-	1227078..1228136 (+)	1059	WP_000649473.1	zinc-dependent alcohol dehydrogenase family protein	-
U0449_RS06165	-	1228287..1228652 (+)	366	WP_000286415.1	DUF1033 family protein	-
U0449_RS06170	comGA/cglA/cilD	1228728..1229669 (+)	942	WP_000249567.1	competence type IV pilus ATPase ComGA	Machinery gene
U0449_RS06175	comGB/cglB	1229617..1230633 (+)	1017	WP_078136505.1	competence type IV pilus assembly protein ComGB	Machinery gene
U0449_RS06180	comGC/cglC	1230635..1230961 (+)	327	WP_000738630.1	comG operon protein ComGC	Machinery gene
U0449_RS06185	comGD/cglD	1230954..1231358 (+)	405	WP_322398090.1	competence type IV pilus minor pilin ComGD	Machinery gene
U0449_RS06190	comGE/cglE	1231321..1231623 (+)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
U0449_RS06195	comGF/cglF	1231586..1232047 (+)	462	WP_000250540.1	competence type IV pilus minor pilin ComGF	Machinery gene
U0449_RS06200	comGG/cglG	1232025..1232438 (+)	414	WP_000265620.1	competence type IV pilus minor pilin ComGG	Machinery gene
U0449_RS06205	-	1232575..1233169 (+)	595	Protein_1212	class I SAM-dependent methyltransferase	-
U0449_RS06210	comYH	1233230..1234183 (+)	954	WP_050219032.1	class I SAM-dependent methyltransferase	Machinery gene
U0449_RS06215	-	1234234..1235424 (+)	1191	WP_000167753.1	acetate kinase	-
U0449_RS06220	-	1235425..1235556 (+)	132	WP_000768904.1	hypothetical protein	-
U0449_RS06225	rnpA	1235573..1235944 (+)	372	WP_000739246.1	ribonuclease P protein component	-
U0449_RS06230	-	1235919..1236743 (+)	825	WP_000727904.1	membrane protein insertase YidC	-

Sequence

Protein

Download Length: 153 a.a. Molecular weight: 17850.40 Da Isoelectric Point: 8.0016

                                >NTDB_id=911497 U0449_RS06195 WP_000250540.1 1231586..1232047(+) (comGF/cglF) [Streptococcus pneumoniae strain 05H0020-2]

MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN

RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEKS

Nucleotide

Download Length: 462 bp

                                >NTDB_id=911497 U0449_RS06195 WP_000250540.1 1231586..1232047(+) (comGF/cglF) [Streptococcus pneumoniae strain 05H0020-2]

ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTACTTGCCCTCAT

TGTCATCAGTGGGGGATTACTCCTTTTTCAAACTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG

AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT

CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG

TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT

TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGF/cglF	Streptococcus pneumoniae Rx1	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae D39	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae R6	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae TIGR4	94.771	100	0.948
comGF/cglF	Streptococcus mitis SK321	93.464	100	0.935
comGF/cglF	Streptococcus mitis NCTC 12261	92.157	100	0.922

Detailed information NTG0911497