Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCU49_RS01905 Genome accession   NZ_AP025282
Coordinates   437412..438575 (+) Length   387 a.a.
NCBI ID   WP_261843341.1    Uniprot ID   -
Organism   Aliamphritea ceti strain KCTC 42154     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 432412..443575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU49_RS01875 rdgB 432651..433247 (-) 597 WP_261843335.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  OCU49_RS01880 - 433271..433732 (-) 462 WP_261843336.1 DUF4426 domain-containing protein -
  OCU49_RS01885 - 433908..434465 (-) 558 WP_261843337.1 YggT family protein -
  OCU49_RS01890 proC 434548..435372 (-) 825 WP_261843338.1 pyrroline-5-carboxylate reductase -
  OCU49_RS01895 - 435425..436129 (-) 705 WP_261843339.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU49_RS01900 pilT 436246..437280 (+) 1035 WP_261843340.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU49_RS01905 pilU 437412..438575 (+) 1164 WP_261843341.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU49_RS01910 - 438640..439254 (-) 615 WP_261843342.1 TetR/AcrR family transcriptional regulator -
  OCU49_RS01915 - 439435..440415 (+) 981 WP_261843343.1 fatty acid desaturase -
  OCU49_RS01920 - 440438..440749 (+) 312 WP_261843344.1 MocE family 2Fe-2S type ferredoxin -
  OCU49_RS01925 - 440875..441432 (+) 558 WP_261843345.1 hypothetical protein -
  OCU49_RS01930 - 441496..441744 (-) 249 WP_261843346.1 YfhL family 4Fe-4S dicluster ferredoxin -
  OCU49_RS01935 coaD 441771..442250 (-) 480 WP_261845298.1 pantetheine-phosphate adenylyltransferase -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43084.13 Da        Isoelectric Point: 5.1530

>NTDB_id=91010 OCU49_RS01905 WP_261843341.1 437412..438575(+) (pilU) [Aliamphritea ceti strain KCTC 42154]
MDITQLLKIMNDRNASDLFVTAGARPSIKVDGTLKPLTKEALKPDQARKLTCSTMNDQQLVEFESTRECNFAISAQGLGR
YRVSAFYQRNSPGMVLRRINTSIPSMEDLSLPPVLKDLAMAKRGLILFVGGTSTGKSTSLASMIDYRNTNSGGHIITIED
PIEYIHDHKQSLITQREVGLDTDSFEVALKNTLRQAPDVILMGEIRTSETMGYGLTFAETGHLCMATMHANNANQALDRI
ISFFPPESHAQVWMDLSLNLKAIVAQQLLPTRDGKGRRAVIEVMINTPLIQDLIRKGEVHELKEVIKKSSNLGMQTFDQA
LYDTYKEGIINYDVALAHADSANDLRLMIKLNASENEVVPDDDTMNFSLQDEDDYIANEGNIQVNKM

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=91010 OCU49_RS01905 WP_261843341.1 437412..438575(+) (pilU) [Aliamphritea ceti strain KCTC 42154]
GTGGATATTACTCAGCTCTTAAAAATTATGAATGATCGCAATGCCTCTGACTTGTTTGTGACCGCAGGTGCCAGGCCGAG
TATCAAGGTGGATGGTACGCTTAAACCATTGACTAAAGAGGCGTTAAAGCCTGACCAGGCCAGAAAGCTGACATGTAGTA
CGATGAATGATCAGCAGCTGGTTGAATTCGAAAGTACCCGTGAGTGTAACTTTGCCATCAGTGCGCAGGGGCTGGGTCGA
TACCGCGTAAGTGCTTTTTATCAGCGGAACTCGCCGGGAATGGTATTGCGTCGGATTAATACCAGTATTCCGAGCATGGA
AGATCTTTCTTTACCGCCGGTGCTGAAAGATCTGGCGATGGCTAAGCGCGGATTGATTCTATTTGTAGGTGGTACCAGTA
CCGGTAAATCCACGTCACTGGCTTCCATGATTGATTACCGTAATACCAATAGCGGTGGTCACATTATTACCATTGAAGAT
CCGATTGAATATATCCATGACCATAAGCAGAGTCTGATTACTCAGCGGGAAGTAGGTCTGGATACAGACTCTTTTGAAGT
CGCGCTGAAGAATACCCTGCGTCAGGCTCCTGATGTTATTTTGATGGGTGAGATTCGTACATCTGAGACGATGGGGTATG
GTCTGACTTTTGCTGAAACCGGTCATCTGTGTATGGCCACCATGCATGCTAACAATGCTAACCAGGCACTGGACCGCATT
ATTAGTTTTTTCCCGCCTGAAAGTCATGCTCAGGTGTGGATGGATTTATCGCTGAACCTTAAGGCTATTGTTGCTCAGCA
GTTGTTGCCAACCCGCGATGGTAAGGGGCGCCGGGCTGTAATTGAAGTAATGATTAATACACCTTTGATTCAAGACCTGA
TCCGTAAAGGCGAAGTACATGAGCTGAAGGAAGTCATTAAAAAGTCTTCTAATCTGGGTATGCAGACCTTTGATCAGGCG
CTGTACGATACCTACAAAGAAGGCATTATTAATTACGATGTGGCGCTGGCGCACGCGGATTCAGCTAATGACCTGCGTTT
GATGATTAAGCTGAATGCGTCTGAGAATGAAGTTGTCCCTGATGACGATACAATGAATTTCTCGTTGCAGGATGAAGATG
ATTACATCGCCAATGAAGGCAATATCCAAGTAAACAAAATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(105-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.839

91.214

0.628
  pilU Acinetobacter baylyi ADP1

59.673

94.832

0.566
  pilU Vibrio cholerae strain A1552

57.143

90.439

0.517
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.953

87.597

0.385
  pilT Legionella pneumophila strain Lp02

42.985

86.563

0.372
  pilT Legionella pneumophila strain ERS1305867

42.985

86.563

0.372
  pilT Acinetobacter baylyi ADP1

41.935

88.114

0.37
  pilT Pseudomonas stutzeri DSM 10701

42.09

86.563

0.364
  pilT Acinetobacter nosocomialis M2

42.09

86.563

0.364
  pilT Acinetobacter baumannii D1279779

42.09

86.563

0.364
  pilT Acinetobacter baumannii strain A118

42.09

86.563

0.364
  pilT Pseudomonas aeruginosa PAK

41.791

86.563

0.362

Multiple sequence alignment