Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCU49_RS01900 Genome accession   NZ_AP025282
Coordinates   436246..437280 (+) Length   344 a.a.
NCBI ID   WP_261843340.1    Uniprot ID   -
Organism   Aliamphritea ceti strain KCTC 42154     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 431246..442280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU49_RS01870 hemW 431446..432603 (-) 1158 WP_261843334.1 radical SAM family heme chaperone HemW -
  OCU49_RS01875 rdgB 432651..433247 (-) 597 WP_261843335.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  OCU49_RS01880 - 433271..433732 (-) 462 WP_261843336.1 DUF4426 domain-containing protein -
  OCU49_RS01885 - 433908..434465 (-) 558 WP_261843337.1 YggT family protein -
  OCU49_RS01890 proC 434548..435372 (-) 825 WP_261843338.1 pyrroline-5-carboxylate reductase -
  OCU49_RS01895 - 435425..436129 (-) 705 WP_261843339.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU49_RS01900 pilT 436246..437280 (+) 1035 WP_261843340.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU49_RS01905 pilU 437412..438575 (+) 1164 WP_261843341.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU49_RS01910 - 438640..439254 (-) 615 WP_261843342.1 TetR/AcrR family transcriptional regulator -
  OCU49_RS01915 - 439435..440415 (+) 981 WP_261843343.1 fatty acid desaturase -
  OCU49_RS01920 - 440438..440749 (+) 312 WP_261843344.1 MocE family 2Fe-2S type ferredoxin -
  OCU49_RS01925 - 440875..441432 (+) 558 WP_261843345.1 hypothetical protein -
  OCU49_RS01930 - 441496..441744 (-) 249 WP_261843346.1 YfhL family 4Fe-4S dicluster ferredoxin -
  OCU49_RS01935 coaD 441771..442250 (-) 480 WP_261845298.1 pantetheine-phosphate adenylyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38051.75 Da        Isoelectric Point: 6.1127

>NTDB_id=91009 OCU49_RS01900 WP_261843340.1 436246..437280(+) (pilT) [Aliamphritea ceti strain KCTC 42154]
MDITELLSFTVQQGASDLHISAGMPPVIRVDGEVRRIKLPSLDHKQVQTLIYDIMNDKQRKDYEDLLETDFSFEVPKLAR
FRVNAFNQNRGASAVFRTIPSKVLTMEDLGLGKVFCDLAEKPRGLVLVTGPTGSGKSTTLAALVDYINETRQDHILTIED
PIEFVHESKKSLINQREVHRDTLGFDEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTVDRI
VDVFPAAEKAMVRSMLSESLQGVISQTLLKKPQGGRVAAHEIMIGTPAIRNLIREDKIAQMYSAIQTGAAFGMVTLDQSL
TDLLSKGLISREAAREKALNPMNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=91009 OCU49_RS01900 WP_261843340.1 436246..437280(+) (pilT) [Aliamphritea ceti strain KCTC 42154]
ATGGATATTACCGAATTACTTTCTTTCACTGTTCAGCAAGGCGCTTCTGATCTACATATTTCTGCAGGCATGCCACCGGT
AATTCGGGTCGATGGTGAAGTCCGGCGTATCAAGCTGCCTTCCCTCGATCATAAACAGGTCCAAACGCTGATTTACGACA
TAATGAATGATAAACAGCGTAAGGACTACGAAGATCTGCTGGAAACAGATTTTTCTTTTGAGGTACCAAAACTGGCCCGT
TTTCGTGTTAACGCTTTTAACCAAAATCGTGGCGCTTCAGCTGTTTTCCGGACCATTCCTTCTAAAGTTTTGACCATGGA
AGACCTGGGGTTGGGTAAAGTATTCTGCGATCTGGCAGAAAAGCCCCGTGGACTGGTATTGGTAACAGGGCCTACTGGCT
CAGGTAAAAGTACCACTTTGGCGGCTTTGGTGGATTATATTAATGAGACCCGTCAGGATCACATTCTTACCATCGAAGAT
CCGATTGAATTTGTTCACGAAAGTAAAAAGAGTCTGATTAACCAGCGGGAAGTACATCGGGATACGTTAGGTTTTGACGA
AGCACTGCGTTCTGCGTTGCGTGAAGACCCTGACGTGATTCTGGTGGGCGAGATGCGGGATTTGGAAACTATTCGTCTGG
CGCTGACTGCCGCGGAAACCGGTCATTTAGTGTTTGGTACCTTGCATACAACGTCTGCAGCCAAAACTGTTGACCGTATT
GTAGATGTGTTCCCTGCGGCGGAGAAAGCCATGGTGCGTTCGATGCTGTCAGAGTCTCTGCAAGGCGTTATCTCTCAGAC
GTTGTTGAAAAAACCACAAGGTGGCCGGGTTGCCGCTCATGAAATAATGATAGGTACTCCGGCAATTCGTAACCTGATTC
GGGAAGACAAAATCGCACAAATGTATTCAGCAATACAAACCGGTGCGGCTTTTGGTATGGTTACTCTTGATCAGTCGCTG
ACTGATTTGCTTAGTAAAGGCCTGATCAGTCGCGAAGCGGCACGTGAAAAGGCACTTAATCCAATGAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

82.267

100

0.823
  pilT Pseudomonas stutzeri DSM 10701

81.686

100

0.817
  pilT Acinetobacter nosocomialis M2

80.814

100

0.808
  pilT Acinetobacter baumannii D1279779

80.523

100

0.805
  pilT Acinetobacter baumannii strain A118

80.523

100

0.805
  pilT Acinetobacter baylyi ADP1

80.233

100

0.802
  pilT Legionella pneumophila strain Lp02

73.256

100

0.733
  pilT Legionella pneumophila strain ERS1305867

73.256

100

0.733
  pilT Neisseria gonorrhoeae MS11

68.328

99.128

0.677
  pilT Neisseria meningitidis 8013

68.328

99.128

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.469

97.965

0.651
  pilT Vibrio cholerae strain A1552

66.469

97.965

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Vibrio cholerae strain A1552

40.708

98.547

0.401
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

40

97.384

0.39
  pilB Acinetobacter baylyi ADP1

32.208

100

0.36

Multiple sequence alignment