Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SIB66_RS02550 Genome accession   NZ_CP138958
Coordinates   470853..471905 (-) Length   350 a.a.
NCBI ID   WP_063407502.1    Uniprot ID   -
Organism   Prochlorococcus sp. MIT 1318     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 465853..476905
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB66_RS02525 - 465949..466365 (+) 417 WP_063421863.1 hypothetical protein -
  SIB66_RS02530 - 466375..466584 (+) 210 WP_063421861.1 hypothetical protein -
  SIB66_RS02535 - 466587..467156 (+) 570 WP_063421859.1 hypothetical protein -
  SIB66_RS02540 pilM 467168..469558 (+) 2391 WP_063421857.1 pilus assembly protein PilM -
  SIB66_RS02545 - 469690..470850 (-) 1161 WP_063421855.1 type II secretion system F family protein -
  SIB66_RS02550 pilT 470853..471905 (-) 1053 WP_063407502.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB66_RS02555 - 471935..473578 (-) 1644 WP_063421853.1 GspE/PulE family protein -
  SIB66_RS02560 - 473657..475009 (-) 1353 WP_063421851.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SIB66_RS02565 - 475216..475701 (-) 486 WP_063421849.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38394.19 Da        Isoelectric Point: 6.5876

>NTDB_id=906245 SIB66_RS02550 WP_063407502.1 470853..471905(-) (pilT) [Prochlorococcus sp. MIT 1318]
MSIARQIIGFAIKAGSSDIHLEEGSPIAIRVHSDIKILENELKPAAMDKLLLEILGQQKVEEFNKTGDLDTSIGLEGLSR
IRINAYVASEKRCLTLRILPENLPKWQDLGLPQPFVDLSEKHRGLVLCTGPTGSGKSTTLAAFVNCILETQKRHILTIED
PIEFEFHHSANSIIHQREVKRDTTSFATALRAALREDPDVIYIGEMRDLETIQLAITAAETGHLVLGTLHTSSAAKTVER
IVDVFPGDQQEQARLQVSTSLLGIMSQTLCKNTAGKRSLAYELLINTPAIGNLIRERKVSQIYSQIQTGSKDGMNTLEQC
LSDLVIKGKISKEEAIGKASIPKAILTEAS

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=906245 SIB66_RS02550 WP_063407502.1 470853..471905(-) (pilT) [Prochlorococcus sp. MIT 1318]
ATGTCTATCGCACGCCAGATTATCGGATTTGCCATCAAGGCTGGCTCCTCCGATATTCACCTCGAAGAAGGCTCACCGAT
TGCGATCCGCGTGCATTCAGATATCAAGATTCTAGAAAATGAGCTTAAGCCTGCCGCTATGGATAAATTACTGCTAGAAA
TTTTAGGTCAGCAGAAAGTAGAGGAGTTCAATAAAACTGGAGATCTTGATACATCGATTGGGCTTGAAGGATTATCAAGA
ATCAGGATTAATGCCTATGTCGCCAGCGAAAAACGCTGTTTAACGTTGCGAATCCTGCCGGAAAACCTGCCAAAATGGCA
AGATTTAGGCCTTCCCCAACCTTTTGTAGACCTTAGTGAAAAACATCGTGGCCTGGTGCTTTGTACAGGCCCAACTGGGT
CAGGTAAGTCAACAACCTTGGCAGCATTTGTGAACTGTATTCTTGAAACACAAAAACGACATATCCTCACGATTGAAGAT
CCGATTGAATTTGAATTTCACCATTCCGCCAACTCCATCATTCATCAACGGGAGGTGAAGCGAGATACAACATCATTCGC
CACAGCGCTGCGCGCCGCATTACGCGAAGATCCAGATGTAATTTACATCGGCGAGATGCGCGATCTGGAAACGATTCAAC
TAGCGATTACAGCAGCAGAAACGGGGCATCTTGTTTTAGGAACACTGCATACCTCATCAGCCGCCAAGACAGTGGAACGC
ATTGTTGATGTCTTCCCCGGCGATCAACAAGAACAAGCCCGTTTACAAGTTTCTACTTCACTGCTCGGAATCATGTCTCA
GACGCTTTGCAAAAACACTGCCGGCAAACGTTCCTTGGCTTATGAATTACTGATTAATACACCTGCTATTGGTAATCTGA
TTCGTGAACGTAAGGTTAGCCAGATCTACTCACAAATCCAAACAGGTAGCAAAGATGGCATGAATACATTGGAGCAATGC
CTTTCTGATCTAGTCATCAAAGGCAAGATCAGCAAAGAAGAAGCGATTGGCAAAGCATCGATTCCTAAGGCAATCCTTAC
TGAAGCGAGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

49.558

96.857

0.48
  pilT Pseudomonas stutzeri DSM 10701

48.688

98

0.477
  pilT Legionella pneumophila strain ERS1305867

47.262

99.143

0.469
  pilT Legionella pneumophila strain Lp02

47.262

99.143

0.469
  pilT Acinetobacter nosocomialis M2

47.661

97.714

0.466
  pilT Acinetobacter baumannii D1279779

47.661

97.714

0.466
  pilT Acinetobacter baumannii strain A118

47.661

97.714

0.466
  pilT Neisseria meningitidis 8013

47.463

95.714

0.454
  pilT Acinetobacter baylyi ADP1

46.087

98.571

0.454
  pilT Neisseria gonorrhoeae MS11

47.164

95.714

0.451
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

46.313

96.857

0.449
  pilT Vibrio cholerae strain A1552

46.313

96.857

0.449
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.018

96.857

0.446