Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SIB41_RS10485 Genome accession   NZ_CP138947
Coordinates   2156452..2157504 (-) Length   350 a.a.
NCBI ID   WP_413358749.1    Uniprot ID   -
Organism   Prochlorococcus sp. MIT 1201     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2151452..2162504
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB41_RS10460 - 2151675..2152091 (+) 417 WP_413358736.1 hypothetical protein -
  SIB41_RS10465 - 2152101..2152310 (+) 210 WP_413358738.1 hypothetical protein -
  SIB41_RS10470 - 2152373..2152882 (+) 510 WP_413358741.1 hypothetical protein -
  SIB41_RS10475 pilM 2152894..2155155 (+) 2262 WP_413358743.1 pilus assembly protein PilM -
  SIB41_RS10480 - 2155289..2156449 (-) 1161 WP_413358746.1 type II secretion system F family protein -
  SIB41_RS10485 pilT 2156452..2157504 (-) 1053 WP_413358749.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB41_RS10490 - 2157534..2159177 (-) 1644 WP_413358752.1 GspE/PulE family protein -
  SIB41_RS10495 - 2159203..2160540 (-) 1338 WP_413358755.1 type IV pilin protein -
  SIB41_RS10500 - 2160676..2161164 (-) 489 WP_413358757.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38457.26 Da        Isoelectric Point: 6.4172

>NTDB_id=906144 SIB41_RS10485 WP_413358749.1 2156452..2157504(-) (pilT) [Prochlorococcus sp. MIT 1201]
MSIARQIIGFAIKAGSSDIHLEEGSPIAIRVHSDIKILENELKPADMDKLMLEILGKEKVEEFNKTGDLDTSIGLEGLSR
IRINAYVASEKRCLTLRILPENLPKWQDLGLPQPFVDLSEKHRGLVLCTGPTGSGKSTTLAAFVNCILETQKRHILTIED
PIEFEFHHSANSIIHQREVKRDTTSFATALRAALREDPDVIYIGEMRDLETIQLAITAAETGHLVLGTLHTSSAAKTVER
IVDVFPGDQQEQARLQVSTSLLGIMSQTLCKNTAGKRSLAYELLINTPAIGNLIRERKVSQIYSQIQTGSKDGMNTLEQC
LSDLVIKGKISKEEAIGKASIPKAILTEAS

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=906144 SIB41_RS10485 WP_413358749.1 2156452..2157504(-) (pilT) [Prochlorococcus sp. MIT 1201]
ATGTCTATCGCACGCCAGATTATCGGATTTGCCATCAAAGCTGGCTCCTCAGATATCCACCTAGAAGAAGGCTCACCGAT
TGCGATCCGCGTGCATTCAGATATCAAGATTCTAGAAAATGAGCTTAAGCCTGCAGATATGGATAAATTAATGCTTGAAA
TTTTAGGTAAGGAGAAAGTAGAGGAGTTCAATAAAACGGGTGATCTCGATACATCGATTGGGCTTGAAGGCTTATCAAGA
ATCAGGATTAATGCCTATGTCGCTAGCGAAAAACGTTGTTTAACATTGCGAATCCTGCCGGAAAACCTGCCAAAATGGCA
GGATTTAGGCCTTCCCCAACCTTTTGTTGACCTTAGTGAGAAACATCGTGGCCTTGTGCTTTGTACAGGCCCAACTGGGT
CGGGTAAGTCAACAACCTTGGCAGCATTTGTGAACTGTATTCTTGAAACACAAAAACGGCATATCCTCACGATTGAAGAT
CCGATTGAATTTGAATTTCATCATTCCGCCAACTCCATCATTCATCAACGGGAGGTAAAGCGCGATACAACATCATTCGC
CACAGCGCTGCGTGCCGCATTACGGGAAGATCCAGATGTGATCTACATCGGCGAGATGCGCGATCTTGAAACGATTCAAC
TCGCGATTACAGCAGCAGAAACGGGGCATCTTGTTTTAGGCACACTGCATACCTCATCCGCCGCCAAGACAGTGGAACGC
ATTGTTGATGTTTTCCCCGGCGATCAACAAGAACAAGCCCGTTTGCAAGTTTCCACTTCACTGCTCGGAATCATGTCTCA
GACGCTTTGCAAAAACACTGCGGGCAAACGCTCCTTGGCTTACGAATTACTCATTAACACCCCTGCTATTGGTAATCTGA
TTCGTGAGCGTAAGGTTAGCCAGATCTACTCACAAATCCAAACAGGTAGTAAAGATGGCATGAATACATTGGAGCAATGC
CTTTCTGATCTAGTCATCAAAGGCAAGATCAGCAAAGAAGAAGCGATTGGCAAAGCATCGATTCCCAAGGCAATCCTTAC
AGAAGCGAGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

49.558

96.857

0.48
  pilT Legionella pneumophila strain ERS1305867

47.55

99.143

0.471
  pilT Legionella pneumophila strain Lp02

47.55

99.143

0.471
  pilT Pseudomonas stutzeri DSM 10701

48.378

96.857

0.469
  pilT Acinetobacter nosocomialis M2

47.953

97.714

0.469
  pilT Acinetobacter baumannii D1279779

47.953

97.714

0.469
  pilT Acinetobacter baumannii strain A118

47.953

97.714

0.469
  pilT Neisseria meningitidis 8013

47.463

95.714

0.454
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

46.608

96.857

0.451
  pilT Vibrio cholerae strain A1552

46.608

96.857

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.608

96.857

0.451
  pilT Neisseria gonorrhoeae MS11

47.164

95.714

0.451
  pilT Acinetobacter baylyi ADP1

45.748

97.429

0.446