Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SBP18_RS19635 Genome accession   NZ_CP138198
Coordinates   4259707..4260843 (-) Length   378 a.a.
NCBI ID   WP_318666199.1    Uniprot ID   -
Organism   Rhodoferax ferrireducens strain YZ-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4254707..4265843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SBP18_RS19600 (SBP18_19600) - 4255268..4255591 (-) 324 WP_011466132.1 EthD family reductase -
  SBP18_RS19605 (SBP18_19605) - 4255684..4256037 (-) 354 WP_318666194.1 MerR family transcriptional regulator -
  SBP18_RS19610 (SBP18_19610) rsmI 4256148..4257083 (-) 936 WP_318666195.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  SBP18_RS19615 (SBP18_19615) - 4257083..4257469 (+) 387 WP_318666196.1 YraN family protein -
  SBP18_RS19620 (SBP18_19620) - 4257506..4258099 (+) 594 WP_011466136.1 phosphoheptose isomerase -
  SBP18_RS19625 (SBP18_19625) - 4258102..4258740 (+) 639 WP_404818502.1 BON domain-containing protein -
  SBP18_RS19630 (SBP18_19630) - 4258784..4259698 (-) 915 WP_318666198.1 NAD(P)-dependent oxidoreductase -
  SBP18_RS19635 (SBP18_19635) pilU 4259707..4260843 (-) 1137 WP_318666199.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SBP18_RS19640 (SBP18_19640) pilT 4260916..4261959 (-) 1044 WP_011466140.1 type IV pilus twitching motility protein PilT Machinery gene
  SBP18_RS19645 (SBP18_19645) - 4261995..4262714 (+) 720 WP_318666200.1 YggS family pyridoxal phosphate-dependent enzyme -
  SBP18_RS19650 (SBP18_19650) metK 4262776..4263957 (-) 1182 WP_318666201.1 methionine adenosyltransferase -
  SBP18_RS19655 (SBP18_19655) - 4264148..4264978 (+) 831 WP_011466143.1 lysophospholipid acyltransferase family protein -
  SBP18_RS19660 (SBP18_19660) - 4265056..4265481 (-) 426 WP_318666202.1 Lrp/AsnC family transcriptional regulator -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41828.21 Da        Isoelectric Point: 6.9020

>NTDB_id=901301 SBP18_RS19635 WP_318666199.1 4259707..4260843(-) (pilU) [Rhodoferax ferrireducens strain YZ-1]
MERDQATKFINDLLKLMISRNGSDLFLTADFPPAVKVDGKVTKVSPQALTAAHTMSLARSIMSDKQVAEFERTKECNFAI
APPGVGRFRVSAFIQQGRVGLVLRVIPLSVPTIDGLGVPVVLKEVATSKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITIEDPIEFVHPHKNCVVTQREVGIDTDSWEAALKNCLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLKALVSQRLIPKQDGRGRYAVVEIMLNTPLVSDMIFKGEVSEIKEIMKKSRNLG
MQTFDQALYDAYEANLITYEDALRNADSLNDLRLQIKLNSHRGKAQDLNAGTENFSIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=901301 SBP18_RS19635 WP_318666199.1 4259707..4260843(-) (pilU) [Rhodoferax ferrireducens strain YZ-1]
ATGGAACGCGATCAAGCCACCAAATTCATCAACGATCTGCTCAAGCTGATGATCAGCCGCAACGGCAGCGATTTGTTCCT
CACGGCCGATTTCCCGCCTGCCGTCAAGGTGGACGGCAAGGTCACCAAGGTCTCGCCCCAAGCGCTTACCGCGGCGCACA
CCATGTCCTTGGCACGCTCCATCATGAGCGACAAGCAGGTGGCCGAGTTTGAACGCACCAAAGAATGCAACTTTGCCATC
GCACCGCCCGGTGTTGGCCGTTTTCGCGTCAGCGCCTTTATCCAGCAGGGCCGGGTTGGCTTGGTGCTGCGGGTCATTCC
GCTGAGCGTGCCCACTATTGACGGCTTGGGTGTGCCTGTGGTGCTCAAGGAGGTGGCTACGTCCAAGCGGGGTCTGTGCA
TTTTGGTGGGAGCCACCGGCTCCGGCAAATCGACCACCCTGGCCGCGATGGTTGATTGGCGCAACGAAAACTCGTTTGGT
CACATCATCACGATTGAAGACCCGATCGAGTTTGTGCATCCGCACAAAAACTGTGTGGTGACCCAGCGCGAGGTTGGTAT
CGACACCGACAGCTGGGAAGCGGCGCTCAAGAATTGCCTGCGCCAGGCGCCCGATGTCATTCTGATGGGCGAGATTCGGG
ACCGCGAAACCATGGAGCATGCGGTCGCGTTTGCCGAGACCGGGCATTTGTGTCTCGCCACCTTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAATTTTTTTCCTGAAGAGCGCCGTGCGCAGTTGCTGATGGACTTGTCGCTCAACCT
GAAGGCGCTGGTGTCGCAGCGGCTGATTCCGAAACAGGATGGGCGCGGGCGCTATGCGGTGGTCGAGATCATGCTCAACA
CGCCGCTCGTCTCCGACATGATCTTCAAGGGCGAAGTCTCCGAGATCAAGGAGATCATGAAGAAAAGCCGCAACCTGGGC
ATGCAGACTTTCGACCAGGCTTTGTACGACGCTTACGAAGCCAACCTGATCACCTATGAGGACGCCCTGCGCAACGCCGA
TTCACTCAATGACCTGCGCCTGCAGATCAAGCTCAACAGCCATCGCGGCAAAGCCCAGGACCTGAACGCCGGTACCGAAA
ATTTCTCCATCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.793

92.063

0.587
  pilU Acinetobacter baylyi ADP1

58.217

94.974

0.553
  pilU Vibrio cholerae strain A1552

53.736

92.063

0.495
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

89.153

0.402
  pilT Pseudomonas aeruginosa PAK

44.214

89.153

0.394
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Vibrio cholerae strain A1552

44.479

83.862

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.479

83.862

0.373
  pilT Acinetobacter baumannii strain A118

43.574

84.392

0.368
  pilT Acinetobacter baumannii D1279779

43.574

84.392

0.368
  pilT Acinetobacter nosocomialis M2

43.574

84.392

0.368
  pilT Legionella pneumophila strain Lp02

41.441

88.095

0.365
  pilT Legionella pneumophila strain ERS1305867

41.441

88.095

0.365