Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R2B70_RS12295 Genome accession   NZ_CP137630
Coordinates   2676101..2677237 (+) Length   378 a.a.
NCBI ID   WP_209792896.1    Uniprot ID   -
Organism   Aeromonas sp. XH     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2671101..2682237
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2B70_RS12265 (R2B70_12265) - 2671178..2671558 (-) 381 WP_042078148.1 diacylglycerol kinase -
  R2B70_RS12270 (R2B70_12270) - 2671700..2672767 (+) 1068 WP_209792890.1 3-deoxy-7-phosphoheptulonate synthase -
  R2B70_RS12275 (R2B70_12275) - 2672978..2673745 (-) 768 WP_412055169.1 ATP-binding cassette domain-containing protein -
  R2B70_RS12280 (R2B70_12280) btuC 2673732..2674736 (-) 1005 WP_318161600.1 vitamin B12 ABC transporter permease BtuC -
  R2B70_RS12285 (R2B70_12285) - 2674813..2675577 (-) 765 WP_318161601.1 transporter substrate-binding domain-containing protein -
  R2B70_RS12290 (R2B70_12290) - 2675688..2676044 (+) 357 WP_010675200.1 DMT family protein -
  R2B70_RS12295 (R2B70_12295) pilU 2676101..2677237 (+) 1137 WP_209792896.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R2B70_RS12300 (R2B70_12300) glgC 2677472..2678746 (+) 1275 WP_318161602.1 glucose-1-phosphate adenylyltransferase -
  R2B70_RS12305 (R2B70_12305) - 2678863..2679105 (-) 243 WP_216990539.1 FeoC-like transcriptional regulator -
  R2B70_RS12310 (R2B70_12310) feoB 2679102..2681372 (-) 2271 WP_216990540.1 Fe(2+) transporter permease subunit FeoB -
  R2B70_RS12315 (R2B70_12315) - 2681369..2681596 (-) 228 WP_041213109.1 FeoA family protein -
  R2B70_RS12320 (R2B70_12320) - 2681716..2682045 (+) 330 WP_318161603.1 stress response translation initiation inhibitor YciH -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42139.15 Da        Isoelectric Point: 6.2536

>NTDB_id=899528 R2B70_RS12295 WP_209792896.1 2676101..2677237(+) (pilU) [Aeromonas sp. XH]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGALRPLTQEPLEPGEVARIADGIMDLEQKQQFEKELEMNLAISLPQTGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHQKCIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLQAFVSQRLVKTTDGGRRAAVEVMLGTHTVRDLIKRGEFGGLKEIMEKSRALGMVTFDSTL
FDLVVEGVIDEEEAVKNADSQNNLRLKIKLWREKGQIGNSAAASGWSLEPTKDEGDLI

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=899528 R2B70_RS12295 WP_209792896.1 2676101..2677237(+) (pilU) [Aeromonas sp. XH]
ATGGAATTGAGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCCGACCTTTATCTCTCGACCGGCGCCCCCCCCTG
CGCCAAGTTCAACGGCGCCCTGCGCCCCCTCACTCAGGAACCGCTCGAACCCGGTGAGGTGGCCCGCATCGCCGACGGCA
TCATGGATCTCGAGCAGAAGCAGCAGTTCGAGAAGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGACAGGCCGT
TTCCGGGTCAACATCTTCAAGCAGCGCAACGAAGTGTCGCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTGAAACTGCCCCCCGTGCTGCTCGACACCATCATGGAGAAGCGCGGCCTGGTGCTGTTCGTCGGCGGCACCGGCT
CGGGCAAGTCTACCTCGCTTGCGGCGCTGATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGCCACCAGAAGTGCATCATCAACCAGCGGGAGGTGGGGGTGGACACCCGCAGCTTCCACGC
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTCATCCTGATCGGCGAGATCCGCGATCGGGAGACCATGGAGCATG
CCCTTGCCTTCTCCGAGACCGGGCATCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGGAGGAGCGCAGGCCCCAGCTCCTCAACGATCTTGGCAACAACCTCCAGGCCTTCGTCTCCCAGCG
GCTGGTGAAGACCACGGATGGGGGGCGCCGGGCGGCGGTGGAGGTCATGCTCGGCACCCACACGGTGCGCGATCTCATCA
AGCGTGGCGAGTTTGGCGGCCTCAAGGAGATCATGGAGAAATCCAGGGCCCTTGGCATGGTCACCTTCGACAGCACCCTG
TTCGATCTGGTGGTGGAGGGGGTGATCGACGAGGAGGAGGCGGTGAAGAACGCCGACTCCCAGAACAACCTGCGCCTCAA
GATCAAGCTGTGGCGAGAGAAGGGACAGATAGGCAACAGCGCGGCGGCCAGCGGCTGGAGCCTGGAGCCCACCAAGGACG
AAGGGGATCTCATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.4

99.206

0.54
  pilU Acinetobacter baylyi ADP1

52.793

94.709

0.5
  pilU Vibrio cholerae strain A1552

49.858

93.386

0.466
  pilT Vibrio cholerae strain A1552

40.741

92.857

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.741

92.857

0.378
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

89.683

0.376
  pilT Legionella pneumophila strain Lp02

42.515

88.36

0.376
  pilT Legionella pneumophila strain ERS1305867

42.515

88.36

0.376
  pilT Pseudomonas aeruginosa PAK

41.279

91.005

0.376
  pilT Pseudomonas stutzeri DSM 10701

40.698

91.005

0.37
  pilT Acinetobacter baumannii strain A118

41.617

88.36

0.368
  pilT Acinetobacter nosocomialis M2

41.617

88.36

0.368
  pilT Acinetobacter baumannii D1279779

41.617

88.36

0.368