Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M2Z51_RS02475 Genome accession   NZ_AP024967
Coordinates   523953..524363 (-) Length   136 a.a.
NCBI ID   WP_148525586.1    Uniprot ID   -
Organism   Vibrio cholerae strain V130003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 518953..529363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2Z51_RS02455 (V130003_04780) coaE 519453..520061 (-) 609 WP_248532070.1 dephospho-CoA kinase -
  M2Z51_RS02460 (V130003_04790) pilD 520058..520933 (-) 876 WP_248532071.1 A24 family peptidase Machinery gene
  M2Z51_RS02465 (V130003_04800) pilC 520991..522217 (-) 1227 WP_248532072.1 type II secretion system F family protein Machinery gene
  M2Z51_RS02470 (V130003_04810) pilB 522260..523948 (-) 1689 WP_153733690.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M2Z51_RS02475 (V130003_04820) pilA 523953..524363 (-) 411 WP_148525586.1 pilin Machinery gene
  M2Z51_RS02480 (V130003_04830) nadC 524608..525498 (-) 891 WP_153733689.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M2Z51_RS02485 (V130003_04840) ampD 525796..526341 (+) 546 WP_000567314.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M2Z51_RS02490 (V130003_04850) fldB 526491..527009 (-) 519 WP_000690112.1 flavodoxin FldB -
  M2Z51_RS02495 (V130003_04860) xerD 527147..528055 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  M2Z51_RS02500 (V130003_04870) dsbC 528072..528824 (+) 753 WP_000115049.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 13943.92 Da        Isoelectric Point: 9.4846

>NTDB_id=89788 M2Z51_RS02475 WP_148525586.1 523953..524363(-) (pilA) [Vibrio cholerae strain V130003]
MKAYKNKQQQGFTLIELMIVVAVIGVLAAIAVPQYQNYVKKSALGTALASASAYKTIVEDSIATKNQFPFISASFGIGTI
RATSGSIATGTDTNDIVASVTQGSGTGSSVTLKRSANGEWKCALSGATDITLGGCS

Nucleotide


Download         Length: 411 bp        

>NTDB_id=89788 M2Z51_RS02475 WP_148525586.1 523953..524363(-) (pilA) [Vibrio cholerae strain V130003]
ATGAAAGCGTATAAAAACAAACAACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTGGTTGCTGTGATTGGTGTCTT
GGCAGCGATTGCAGTGCCTCAATATCAGAACTATGTCAAGAAAAGTGCTTTAGGAACCGCATTGGCTTCAGCATCTGCCT
ATAAAACGATTGTTGAAGACAGTATTGCAACAAAAAATCAGTTTCCATTCATATCGGCATCATTTGGTATAGGTACAATC
CGTGCAACGTCAGGATCTATTGCAACAGGGACTGACACTAACGATATCGTTGCAAGTGTTACACAAGGCTCTGGTACTGG
CTCTAGCGTTACTTTAAAACGGAGCGCAAATGGTGAATGGAAATGTGCTCTATCCGGTGCAACCGATATTACTTTAGGTG
GATGTTCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(41-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

48.993

100

0.537
  pilA Vibrio cholerae strain A1552

48.993

100

0.537
  pilA Vibrio cholerae C6706

48.993

100

0.537
  pilA Pseudomonas aeruginosa PAK

41.176

100

0.463
  pilA Vibrio parahaemolyticus RIMD 2210633

42.759

100

0.456
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.406

100

0.39
  pilA Acinetobacter baumannii strain A118

37.956

100

0.382
  pilA2 Legionella pneumophila str. Paris

41.667

88.235

0.368
  pilA2 Legionella pneumophila strain ERS1305867

40.833

88.235

0.36
  pilA Glaesserella parasuis strain SC1401

36.296

99.265

0.36

Multiple sequence alignment