Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M2Z51_RS02470 Genome accession   NZ_AP024967
Coordinates   522260..523948 (-) Length   562 a.a.
NCBI ID   WP_153733690.1    Uniprot ID   -
Organism   Vibrio cholerae strain V130003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 517260..528948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2Z51_RS02445 (V130003_04760) yacG 518342..518539 (-) 198 WP_000162863.1 DNA gyrase inhibitor YacG -
  M2Z51_RS02450 (V130003_04770) zapD 518681..519421 (-) 741 WP_000207194.1 cell division protein ZapD -
  M2Z51_RS02455 (V130003_04780) coaE 519453..520061 (-) 609 WP_248532070.1 dephospho-CoA kinase -
  M2Z51_RS02460 (V130003_04790) pilD 520058..520933 (-) 876 WP_248532071.1 A24 family peptidase Machinery gene
  M2Z51_RS02465 (V130003_04800) pilC 520991..522217 (-) 1227 WP_248532072.1 type II secretion system F family protein Machinery gene
  M2Z51_RS02470 (V130003_04810) pilB 522260..523948 (-) 1689 WP_153733690.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M2Z51_RS02475 (V130003_04820) pilA 523953..524363 (-) 411 WP_148525586.1 pilin Machinery gene
  M2Z51_RS02480 (V130003_04830) nadC 524608..525498 (-) 891 WP_153733689.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M2Z51_RS02485 (V130003_04840) ampD 525796..526341 (+) 546 WP_000567314.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M2Z51_RS02490 (V130003_04850) fldB 526491..527009 (-) 519 WP_000690112.1 flavodoxin FldB -
  M2Z51_RS02495 (V130003_04860) xerD 527147..528055 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  M2Z51_RS02500 (V130003_04870) dsbC 528072..528824 (+) 753 WP_000115049.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62166.31 Da        Isoelectric Point: 6.2600

>NTDB_id=89787 M2Z51_RS02470 WP_153733690.1 522260..523948(-) (pilB) [Vibrio cholerae strain V130003]
MLTNLVAILRQAELISAMQEQAVVTQVSASGTSVPEALLGLGFFHPRELTEQLSHIFGLPETDLSRYDYANLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAN
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=89787 M2Z51_RS02470 WP_153733690.1 522260..523948(-) (pilB) [Vibrio cholerae strain V130003]
ATGCTCACCAACCTTGTTGCTATCTTGCGTCAGGCTGAGTTAATCAGCGCGATGCAAGAACAAGCGGTGGTTACCCAGGT
TAGCGCTTCGGGGACGTCAGTACCCGAGGCGCTGCTTGGTTTAGGTTTTTTTCACCCCCGAGAACTCACCGAACAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGACCTTAGCCGCTACGACTACGCCAACTTGTGCCAACAGCTCGGGCTGCGTGAA
CTGATCACCCGCTACGATGCCTTGCCGATTGCCAAGCAAGGCAATTTACTGCTGCTGGCGGTCTCTGACCCAACCTTACT
GCAAGCCGAAGAAGAGTTTCGCTTTGCCACAGGATTACAAGTCGAACTGGCACTGGCCGATCACCGCGCGCTGCAAGCCG
CGATTCGCCGTTTGTATGGCAGATCTATTCAAGGCGCAGCCAACCAAGGGAAAGAGATCAGCCAAGATGAGCTCGCCAAT
CTGGTTAAAGTCAGTGATGACGAGCTGCAATCGATTGAAGATCTCAGCCAAGATGACTCGCCGGTTAGCCGCTTTATCAA
CCAAGTGCTGCTCGATGCGGTACGTAAAGGTGCCTCGGATATTCATTTTGAGCCTTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGGATCTTGGTCGAAACTCAGCAACCGGCTAGCCATTTAAGCCGCCGTTTAGCCGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGCTTGCCACAAGATGGCCGGATTAAACTGCGCCTAAGCCGCGATACCGCCAT
TGATATGCGTGTATCCACGCTTCCCACTTTATGGGGAGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTATCTCAATGCCCTCAAAAGACCGCAAGGGATGATC
TTGATGACCGGCCCCACCGGCAGCGGCAAAACCGTTTCGCTCTATACTGGGCTACGCATTCTCAACACGTCACAGATCAA
TATCTCCACCGCGGAAGATCCGGTAGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAGCCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTGCGCCAAGACCCGGATGTGGTGATGGTCGGCGAAATACGCGATCTGGAAACC
GCCGAAATCGCGGTCAAAGCCGCGCAAACCGGCCACTTGGTGCTTTCAACACTGCACACCAACTCGGCCGCTGAAACCGT
GATTCGCTTAGCCAATATGGGGGTGGAGCCGTTTAACCTCGCGTCATCACTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCTTGTGTAAACATTGCAAAATCGCGGTGCGCCCTTCCGCCCTATTGCAAAGCCAATTTGCATTTCAGCCCAATGAA
ATCTTGTATGAAGCCAATGCGGCGGGGTGTAACGAGTGTACGGGCGGCTATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAACGCGCCAGCATTCATCAAATTGAACGTTTAGCTAAAGCCAATG
GTATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTGCAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

98.932

100

0.989
  pilB Vibrio parahaemolyticus RIMD 2210633

74.021

100

0.74
  pilB Vibrio campbellii strain DS40M4

73.843

100

0.738
  pilB Legionella pneumophila strain ERS1305867

52.838

90.925

0.48
  pilB Acinetobacter baylyi ADP1

50.385

92.527

0.466
  pilB Acinetobacter baumannii D1279779

50.192

92.527

0.464
  pilF Neisseria gonorrhoeae MS11

45.73

100

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

36.65

100

0.393
  pilF Thermus thermophilus HB27

39.668

96.441

0.383
  pilB Haemophilus influenzae 86-028NP

39.469

93.772

0.37

Multiple sequence alignment