Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   R4701_RS06290 Genome accession   NZ_CP137114
Coordinates   1250318..1251259 (+) Length   313 a.a.
NCBI ID   WP_000249571.1    Uniprot ID   A0A0B7M3Q7
Organism   Streptococcus pneumoniae strain 15P3054     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1245318..1256259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4701_RS06270 - 1245384..1247201 (+) 1818 WP_001220871.1 acyltransferase family protein -
  R4701_RS06275 nagA 1247354..1248505 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  R4701_RS06280 - 1248668..1249726 (+) 1059 WP_000649466.1 zinc-dependent alcohol dehydrogenase family protein -
  R4701_RS06285 - 1249877..1250242 (+) 366 WP_000286415.1 DUF1033 family protein -
  R4701_RS06290 comGA/cglA/cilD 1250318..1251259 (+) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  R4701_RS06295 comGB/cglB 1251207..1252223 (+) 1017 WP_075218762.1 competence type IV pilus assembly protein ComGB Machinery gene
  R4701_RS06300 comGC/cglC 1252225..1252551 (+) 327 WP_000738630.1 comG operon protein ComGC Machinery gene
  R4701_RS06305 comGD/cglD 1252544..1252948 (+) 405 WP_000588023.1 competence type IV pilus minor pilin ComGD Machinery gene
  R4701_RS06310 comGE/cglE 1252911..1253213 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R4701_RS06315 comGF/cglF 1253176..1253637 (+) 462 WP_000250540.1 competence type IV pilus minor pilin ComGF Machinery gene
  R4701_RS06320 comGG/cglG 1253615..1254028 (+) 414 WP_000265620.1 competence type IV pilus minor pilin ComGG Machinery gene
  R4701_RS06325 - 1254165..1254759 (+) 595 Protein_1234 class I SAM-dependent methyltransferase -
  R4701_RS06330 comYH 1254820..1255773 (+) 954 WP_000345126.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35591.51 Da        Isoelectric Point: 6.0083

>NTDB_id=896184 R4701_RS06290 WP_000249571.1 1250318..1251259(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 15P3054]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=896184 R4701_RS06290 WP_000249571.1 1250318..1251259(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 15P3054]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATTTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGACATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGCCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7M3Q7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.042

100

0.99
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.805

100

0.968
  comYA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

42.642

84.665

0.361