NTDB

Overview

Name	comGF/cglF	Type	Machinery gene
Locus tag	R4701_RS06315	Genome accession	NZ_CP137114
Coordinates	1253176..1253637 (+)	Length	153 a.a.
NCBI ID	WP_000250540.1	Uniprot ID	-
Organism	Streptococcus pneumoniae strain 15P3054
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1248176..1258637

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R4701_RS06280	-	1248668..1249726 (+)	1059	WP_000649466.1	zinc-dependent alcohol dehydrogenase family protein	-
R4701_RS06285	-	1249877..1250242 (+)	366	WP_000286415.1	DUF1033 family protein	-
R4701_RS06290	comGA/cglA/cilD	1250318..1251259 (+)	942	WP_000249571.1	competence type IV pilus ATPase ComGA	Machinery gene
R4701_RS06295	comGB/cglB	1251207..1252223 (+)	1017	WP_075218762.1	competence type IV pilus assembly protein ComGB	Machinery gene
R4701_RS06300	comGC/cglC	1252225..1252551 (+)	327	WP_000738630.1	comG operon protein ComGC	Machinery gene
R4701_RS06305	comGD/cglD	1252544..1252948 (+)	405	WP_000588023.1	competence type IV pilus minor pilin ComGD	Machinery gene
R4701_RS06310	comGE/cglE	1252911..1253213 (+)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
R4701_RS06315	comGF/cglF	1253176..1253637 (+)	462	WP_000250540.1	competence type IV pilus minor pilin ComGF	Machinery gene
R4701_RS06320	comGG/cglG	1253615..1254028 (+)	414	WP_000265620.1	competence type IV pilus minor pilin ComGG	Machinery gene
R4701_RS06325	-	1254165..1254759 (+)	595	Protein_1234	class I SAM-dependent methyltransferase	-
R4701_RS06330	comYH	1254820..1255773 (+)	954	WP_000345126.1	class I SAM-dependent methyltransferase	Machinery gene
R4701_RS06335	-	1255824..1257014 (+)	1191	WP_000167757.1	acetate kinase	-
R4701_RS06340	-	1257015..1257146 (+)	132	WP_000768904.1	hypothetical protein	-
R4701_RS06345	rnpA	1257163..1257534 (+)	372	WP_000739246.1	ribonuclease P protein component	-
R4701_RS06350	-	1257509..1258333 (+)	825	WP_000727904.1	membrane protein insertase YidC	-

Sequence

Protein

Download Length: 153 a.a. Molecular weight: 17850.40 Da Isoelectric Point: 8.0016

                                >NTDB_id=896189 R4701_RS06315 WP_000250540.1 1253176..1253637(+) (comGF/cglF) [Streptococcus pneumoniae strain 15P3054]

MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN

RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEKS

Nucleotide

Download Length: 462 bp

                                >NTDB_id=896189 R4701_RS06315 WP_000250540.1 1253176..1253637(+) (comGF/cglF) [Streptococcus pneumoniae strain 15P3054]

ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTACTTGCCCTCAT

TGTCATCAGTGGGGGATTACTCCTTTTTCAAACTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG

AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT

CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG

TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT

TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGF/cglF	Streptococcus pneumoniae Rx1	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae D39	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae R6	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae TIGR4	94.771	100	0.948
comGF/cglF	Streptococcus mitis SK321	93.464	100	0.935
comGF/cglF	Streptococcus mitis NCTC 12261	92.157	100	0.922

Detailed information NTG0896189