Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   R4706_RS06320 Genome accession   NZ_CP137111
Coordinates   1261412..1262353 (+) Length   313 a.a.
NCBI ID   WP_317815369.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 17023     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1256412..1267353
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4706_RS06300 - 1256482..1258299 (+) 1818 WP_044727684.1 acyltransferase family protein -
  R4706_RS06305 nagA 1258452..1259603 (+) 1152 WP_044727685.1 N-acetylglucosamine-6-phosphate deacetylase -
  R4706_RS06310 - 1259762..1260820 (+) 1059 WP_044727686.1 zinc-dependent alcohol dehydrogenase family protein -
  R4706_RS06315 - 1260971..1261336 (+) 366 WP_000286415.1 DUF1033 family protein -
  R4706_RS06320 comGA/cglA/cilD 1261412..1262353 (+) 942 WP_317815369.1 competence type IV pilus ATPase ComGA Machinery gene
  R4706_RS06325 comGB/cglB 1262301..1263317 (+) 1017 WP_078102664.1 competence type IV pilus assembly protein ComGB Machinery gene
  R4706_RS06330 comGC/cglC 1263319..1263645 (+) 327 WP_000738627.1 comG operon protein ComGC Machinery gene
  R4706_RS06335 comGD/cglD 1263638..1264042 (+) 405 WP_000588018.1 competence type IV pilus minor pilin ComGD Machinery gene
  R4706_RS06340 comGE/cglE 1264005..1264307 (+) 303 WP_257890291.1 competence type IV pilus minor pilin ComGE Machinery gene
  R4706_RS06345 comGF/cglF 1264270..1264731 (+) 462 WP_044727689.1 competence type IV pilus minor pilin ComGF Machinery gene
  R4706_RS06350 comGG/cglG 1264709..1265122 (+) 414 WP_000265624.1 competence type IV pilus minor pilin ComGG Machinery gene
  R4706_RS06355 - 1265259..1265853 (+) 595 Protein_1240 class I SAM-dependent methyltransferase -
  R4706_RS06360 comYH 1265914..1266867 (+) 954 WP_044727690.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35604.49 Da        Isoelectric Point: 5.8185

>NTDB_id=895953 R4706_RS06320 WP_317815369.1 1261412..1262353(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 17023]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTDATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=895953 R4706_RS06320 WP_317815369.1 1261412..1262353(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 17023]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATCTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATAGCGTCTCTACGTTTATCTACTGTAGGTGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACCTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGATGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.361

100

0.994
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

95.847

100

0.958
  comYA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comYA Streptococcus mutans UA159

65.595

99.361

0.652
  comYA Streptococcus mutans UA140

65.595

99.361

0.652
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.258

99.042

0.617
  comGA Lactococcus lactis subsp. cremoris KW2

54.487

99.681

0.543