Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   R4706_RS06325 Genome accession   NZ_CP137111
Coordinates   1262301..1263317 (+) Length   338 a.a.
NCBI ID   WP_078102664.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 17023     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1257301..1268317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4706_RS06305 nagA 1258452..1259603 (+) 1152 WP_044727685.1 N-acetylglucosamine-6-phosphate deacetylase -
  R4706_RS06310 - 1259762..1260820 (+) 1059 WP_044727686.1 zinc-dependent alcohol dehydrogenase family protein -
  R4706_RS06315 - 1260971..1261336 (+) 366 WP_000286415.1 DUF1033 family protein -
  R4706_RS06320 comGA/cglA/cilD 1261412..1262353 (+) 942 WP_317815369.1 competence type IV pilus ATPase ComGA Machinery gene
  R4706_RS06325 comGB/cglB 1262301..1263317 (+) 1017 WP_078102664.1 competence type IV pilus assembly protein ComGB Machinery gene
  R4706_RS06330 comGC/cglC 1263319..1263645 (+) 327 WP_000738627.1 comG operon protein ComGC Machinery gene
  R4706_RS06335 comGD/cglD 1263638..1264042 (+) 405 WP_000588018.1 competence type IV pilus minor pilin ComGD Machinery gene
  R4706_RS06340 comGE/cglE 1264005..1264307 (+) 303 WP_257890291.1 competence type IV pilus minor pilin ComGE Machinery gene
  R4706_RS06345 comGF/cglF 1264270..1264731 (+) 462 WP_044727689.1 competence type IV pilus minor pilin ComGF Machinery gene
  R4706_RS06350 comGG/cglG 1264709..1265122 (+) 414 WP_000265624.1 competence type IV pilus minor pilin ComGG Machinery gene
  R4706_RS06355 - 1265259..1265853 (+) 595 Protein_1240 class I SAM-dependent methyltransferase -
  R4706_RS06360 comYH 1265914..1266867 (+) 954 WP_044727690.1 class I SAM-dependent methyltransferase Machinery gene
  R4706_RS06365 - 1266918..1268108 (+) 1191 WP_000167765.1 acetate kinase -
  R4706_RS06370 - 1268109..1268240 (+) 132 WP_000768904.1 hypothetical protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38623.69 Da        Isoelectric Point: 9.4800

>NTDB_id=895954 R4706_RS06325 WP_078102664.1 1262301..1263317(+) (comGB/cglB) [Streptococcus pneumoniae strain 17023]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRIGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMIVFVSVGILLALTFYKRSSKMRVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLDSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=895954 R4706_RS06325 WP_078102664.1 1262301..1263317(+) (comGB/cglB) [Streptococcus pneumoniae strain 17023]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTATAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCATCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCCAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCACAAATCTTT
CTAGGAATGATAGTTTTTGTTTCAGTAGGTATCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGCGCGTCTT
TTCTATCTTAGCGCGCCTTCCTTTTATTGGAATCTTTGTGCAGACCTATTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGTTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGATAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

97.337

100

0.973
  comGB/cglB Streptococcus pneumoniae D39

97.337

100

0.973
  comGB/cglB Streptococcus pneumoniae R6

97.337

100

0.973
  comGB/cglB Streptococcus pneumoniae TIGR4

97.337

100

0.973
  comGB/cglB Streptococcus mitis NCTC 12261

94.97

100

0.95
  comGB/cglB Streptococcus mitis SK321

94.379

100

0.944
  comYB Streptococcus gordonii str. Challis substr. CH1

70.536

99.408

0.701
  comYB Streptococcus mutans UA140

58.307

94.379

0.55
  comYB Streptococcus mutans UA159

58.307

94.379

0.55
  comGB Lactococcus lactis subsp. cremoris KW2

50

98.817

0.494