Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LDN97_RS08700 Genome accession   NZ_AP024940
Coordinates   1786143..1787279 (-) Length   378 a.a.
NCBI ID   WP_161653005.1    Uniprot ID   -
Organism   Aeromonas caviae strain KAM339     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1781143..1792279
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDN97_RS08675 (KAM339_016720) - 1782038..1782595 (-) 558 WP_010675206.1 TMEM165/GDT1 family protein -
  LDN97_RS08680 (KAM339_016730) - 1782849..1783178 (-) 330 WP_042864056.1 translation initiation factor -
  LDN97_RS08685 (KAM339_016740) - 1783298..1783525 (+) 228 WP_010675204.1 FeoA family protein -
  LDN97_RS08690 (KAM339_016750) feoB 1783522..1785792 (+) 2271 WP_223956518.1 Fe(2+) transporter permease subunit FeoB -
  LDN97_RS08695 (KAM339_016760) - 1785839..1786081 (+) 243 WP_039041210.1 FeoC-like transcriptional regulator -
  LDN97_RS08700 (KAM339_016770) pilU 1786143..1787279 (-) 1137 WP_161653005.1 type IVa pilus ATPase TapW Machinery gene
  LDN97_RS08705 (KAM339_016780) - 1787336..1787692 (-) 357 WP_010675200.1 DMT family protein -
  LDN97_RS08710 (KAM339_016790) - 1787805..1788536 (+) 732 WP_223956519.1 substrate-binding periplasmic protein -
  LDN97_RS08715 (KAM339_016800) btuC 1788612..1789619 (+) 1008 WP_223956520.1 vitamin B12 ABC transporter permease BtuC -
  LDN97_RS08720 (KAM339_016810) - 1789606..1790379 (+) 774 WP_223956718.1 ATP-binding cassette domain-containing protein -
  LDN97_RS08725 (KAM339_016820) - 1790620..1791687 (-) 1068 WP_041213104.1 3-deoxy-7-phosphoheptulonate synthase -
  LDN97_RS08730 (KAM339_016830) - 1791829..1792209 (+) 381 WP_041213103.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42275.33 Da        Isoelectric Point: 6.4280

>NTDB_id=89586 LDN97_RS08700 WP_161653005.1 1786143..1787279(-) (pilU) [Aeromonas caviae strain KAM339]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGALRPLTQEPLEPGEVARIAYDIMDLEQKQQFEKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHQKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEVMLGTHTVRDLIKRGEFGGLKEIMEKSRALGMVTFDSTL
FDLVVEGVIDEEEAVKNADSQNNLRLKIKLWREKGQIGNSAAASGWSLEPTKDEGDLF

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=89586 LDN97_RS08700 WP_161653005.1 1786143..1787279(-) (pilU) [Aeromonas caviae strain KAM339]
ATGGAATTGAGAGACATGCTGCAGATCCTGGCCAAACAGGACGGTTCCGATCTCTATCTCTCCACCGGCGCCCCCCCCTG
CGCCAAGTTCAACGGCGCTCTGCGCCCCCTCACCCAGGAGCCCCTGGAGCCGGGCGAGGTGGCCCGCATCGCCTACGACA
TCATGGATCTCGAGCAGAAACAGCAGTTCGAGAAAGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGCCGT
TTTCGGGTCAACATCTTCAAGCAGCGCAATGAAGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTCAAACTCCCTCCGGTGCTCCTCGACACCATCATGGAGAAGCGGGGTCTGGTGCTCTTCGTCGGCGGCACGGGGT
CCGGCAAGTCCACGTCGCTCGCGGCCCTCATCGATCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGCCACCAGAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTCATCCTCATCGGTGAAATCCGCGATCGGGAGACCATGGAGCATG
CCCTCGCCTTCTCGGAAACCGGGCATCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGGAAGAGCGCAGACCCCAACTGCTCAACGACCTTGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
GCTGGTGAAGACCACGGACGGCGGACGCCGGGCGGCGGTGGAGGTGATGCTCGGAACCCACACGGTGCGCGATCTCATCA
AGCGCGGCGAGTTTGGTGGCCTCAAGGAGATCATGGAAAAATCCAGGGCCCTTGGCATGGTGACCTTCGACAGTACCCTG
TTCGATCTGGTGGTCGAGGGGGTGATCGACGAGGAGGAGGCGGTGAAGAACGCGGACTCCCAGAACAACCTGCGCCTCAA
GATCAAACTCTGGCGGGAGAAGGGCCAGATTGGCAACAGCGCGGCGGCGAGCGGCTGGAGCCTGGAGCCCACCAAGGACG
AGGGCGACCTCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.4

99.206

0.54
  pilU Acinetobacter baylyi ADP1

52.514

94.709

0.497
  pilU Vibrio cholerae strain A1552

49.858

93.386

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.478

89.683

0.381
  pilT Vibrio cholerae strain A1552

40.456

92.857

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.456

92.857

0.376
  pilT Legionella pneumophila strain ERS1305867

42.515

88.36

0.376
  pilT Legionella pneumophila strain Lp02

42.515

88.36

0.376
  pilT Pseudomonas aeruginosa PAK

41.279

91.005

0.376
  pilT Pseudomonas stutzeri DSM 10701

40.698

91.005

0.37
  pilT Acinetobacter baumannii strain A118

41.617

88.36

0.368
  pilT Acinetobacter nosocomialis M2

41.617

88.36

0.368
  pilT Acinetobacter baumannii D1279779

41.617

88.36

0.368

Multiple sequence alignment