Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   R4710_RS04585 Genome accession   NZ_CP137101
Coordinates   853381..854322 (-) Length   313 a.a.
NCBI ID   WP_000249571.1    Uniprot ID   A0A0B7M3Q7
Organism   Streptococcus pneumoniae strain 16P2012-2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 848381..859322
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4710_RS04545 comYH 848867..849820 (-) 954 WP_000345130.1 class I SAM-dependent methyltransferase Machinery gene
  R4710_RS04550 - 849881..850475 (-) 595 Protein_869 class I SAM-dependent methyltransferase -
  R4710_RS04555 comGG/cglG 850612..851025 (-) 414 WP_000265631.1 competence type IV pilus minor pilin ComGG Machinery gene
  R4710_RS04560 comGF/cglF 851003..851464 (-) 462 WP_000250531.1 competence type IV pilus minor pilin ComGF Machinery gene
  R4710_RS04565 comGE/cglE 851427..851729 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R4710_RS04570 comGD/cglD 851692..852096 (-) 405 WP_000588020.1 competence type IV pilus minor pilin ComGD Machinery gene
  R4710_RS04575 comGC/cglC 852089..852415 (-) 327 WP_000738632.1 comG operon protein ComGC Machinery gene
  R4710_RS04580 comGB/cglB 852417..853433 (-) 1017 WP_075218762.1 competence type IV pilus assembly protein ComGB Machinery gene
  R4710_RS04585 comGA/cglA/cilD 853381..854322 (-) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  R4710_RS04590 - 854398..854763 (-) 366 WP_000286415.1 DUF1033 family protein -
  R4710_RS04595 - 854914..855972 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  R4710_RS04600 nagA 856135..857286 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  R4710_RS04605 - 857439..859256 (-) 1818 WP_317805251.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35591.51 Da        Isoelectric Point: 6.0083

>NTDB_id=895181 R4710_RS04585 WP_000249571.1 853381..854322(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 16P2012-2]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=895181 R4710_RS04585 WP_000249571.1 853381..854322(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 16P2012-2]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATTTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGACATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGCCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7M3Q7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.042

100

0.99
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.805

100

0.968
  comYA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

42.642

84.665

0.361