Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   R4710_RS04580 Genome accession   NZ_CP137101
Coordinates   852417..853433 (-) Length   338 a.a.
NCBI ID   WP_075218762.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 16P2012-2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 847417..858433
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4710_RS04535 - 847494..847625 (-) 132 WP_000768904.1 hypothetical protein -
  R4710_RS04540 - 847626..848816 (-) 1191 WP_000167766.1 acetate kinase -
  R4710_RS04545 comYH 848867..849820 (-) 954 WP_000345130.1 class I SAM-dependent methyltransferase Machinery gene
  R4710_RS04550 - 849881..850475 (-) 595 Protein_869 class I SAM-dependent methyltransferase -
  R4710_RS04555 comGG/cglG 850612..851025 (-) 414 WP_000265631.1 competence type IV pilus minor pilin ComGG Machinery gene
  R4710_RS04560 comGF/cglF 851003..851464 (-) 462 WP_000250531.1 competence type IV pilus minor pilin ComGF Machinery gene
  R4710_RS04565 comGE/cglE 851427..851729 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R4710_RS04570 comGD/cglD 851692..852096 (-) 405 WP_000588020.1 competence type IV pilus minor pilin ComGD Machinery gene
  R4710_RS04575 comGC/cglC 852089..852415 (-) 327 WP_000738632.1 comG operon protein ComGC Machinery gene
  R4710_RS04580 comGB/cglB 852417..853433 (-) 1017 WP_075218762.1 competence type IV pilus assembly protein ComGB Machinery gene
  R4710_RS04585 comGA/cglA/cilD 853381..854322 (-) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  R4710_RS04590 - 854398..854763 (-) 366 WP_000286415.1 DUF1033 family protein -
  R4710_RS04595 - 854914..855972 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  R4710_RS04600 nagA 856135..857286 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38478.51 Da        Isoelectric Point: 9.6073

>NTDB_id=895180 R4710_RS04580 WP_075218762.1 852417..853433(-) (comGB/cglB) [Streptococcus pneumoniae strain 16P2012-2]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVIQMRAGLSQGKSFSEMMESLGFSSTI
ITQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=895180 R4710_RS04580 WP_075218762.1 852417..853433(-) (comGB/cglB) [Streptococcus pneumoniae strain 16P2012-2]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGATCCAGATGCGTGCGGGCTTGTCTCAAGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATTTTCAAGCACCATT
ATCACTCAGTTATCCCTAGCTGAAGTTCATGGAAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCTAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae D39

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae R6

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae TIGR4

98.225

100

0.982
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

71.131

99.408

0.707
  comYB Streptococcus mutans UA140

57.862

94.083

0.544
  comYB Streptococcus mutans UA159

57.862

94.083

0.544
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503