Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R1T10_RS08460 Genome accession   NZ_CP136807
Coordinates   1683666..1684796 (-) Length   376 a.a.
NCBI ID   WP_002214012.1    Uniprot ID   X5EQV8
Organism   Neisseria meningitidis NMB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1678666..1689796
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R1T10_RS08420 - 1678964..1679386 (-) 423 WP_002226495.1 protein Gly1ORF1 -
  R1T10_RS08425 - 1679789..1680109 (-) 321 WP_002214001.1 DUF2322 family protein -
  R1T10_RS08430 upp 1680132..1680758 (-) 627 WP_002214002.1 uracil phosphoribosyltransferase -
  R1T10_RS08435 grxD 1680864..1681175 (-) 312 WP_002214004.1 Grx4 family monothiol glutaredoxin -
  R1T10_RS08440 - 1681276..1681491 (-) 216 WP_002219531.1 DUF333 domain-containing protein -
  R1T10_RS08445 - 1681517..1682296 (-) 780 WP_017201076.1 TatD family hydrolase -
  R1T10_RS08450 - 1682301..1682651 (-) 351 WP_002221202.1 PilZ domain-containing protein -
  R1T10_RS08455 - 1682655..1683632 (-) 978 WP_002226497.1 DNA polymerase III subunit delta' -
  R1T10_RS08460 pilU 1683666..1684796 (-) 1131 WP_002214012.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R1T10_RS08465 - 1684964..1685665 (+) 702 WP_002226498.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  R1T10_RS08470 lepA 1685807..1687600 (+) 1794 WP_002226499.1 translation elongation factor 4 -
  R1T10_RS08475 lepB 1687743..1688762 (+) 1020 WP_002217606.1 signal peptidase I -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42215.36 Da        Isoelectric Point: 6.4333

>NTDB_id=892863 R1T10_RS08460 WP_002214012.1 1683666..1684796(-) (pilU) [Neisseria meningitidis NMB]
MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGDISLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=892863 R1T10_RS08460 WP_002214012.1 1683666..1684796(-) (pilU) [Neisseria meningitidis NMB]
ATGTTTACCGATGAAAATATGACCGCAAAGGAAGAACTGTTCGCATGGCTGCGCCATATGAACAAAAACAAAGGTTCCGA
CCTGTTTGTGACGACCCATTTCCCGCCCGCGATGAAGCTGGACGGCAAAATCACCCGCATCACGGACGAACCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGCCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGCGCGACGGCGTTGGTATTCCG
TGCGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTCAACCTGCCGCCGGTCTTGAAGGATGTCGCGCTGAAAAAACGCG
GGCTGGTTATTTTTGTCGGCGGCACCGGCTCGGGCAAATCGACTTCGCTTGCCTCGCTTATCGATTACCGCAATGAAAAT
TCGTTCGGACACATTATCACCATCGAAGACCCGATCGAGTTTGTCCACGAACACAAAAACTGCATCATCACCCAGCGAGA
GGTCGGCGTGGATACGGAAAACTGGATGGCGGCGTTGAAAAACACGCTGCGTCAGGCGCCCGATGTCATCCTTATCGGCG
AAATCCGCGACCGCGAAACAATGGACTACGCCATTGCCTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCACTCGACCGCATCATCAACTTTTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCGTTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTGC
TGCTCAATTCGCCCCTGATTTCGGAGTTGATTCACAACGGCAACATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCCTGGGTATGCAGACCTTCGATCAACACCTTTACCAATTGTATGAAAAAGGCGATATTTCCCTGCAAGAAGCATTGAA
AAATGCCGATTCCGCACACGATTTGCGTTTGGCGGTACAGTTGCGCAGCCGCCGCGCGCAAAGTTCCAGCCCCGATTTGG
AACTGCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB X5EQV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.375

93.617

0.556
  pilU Acinetobacter baylyi ADP1

57.434

91.223

0.524
  pilU Vibrio cholerae strain A1552

54.366

94.415

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.343

93.883

0.407
  pilT Legionella pneumophila strain Lp02

43.827

86.17

0.378
  pilT Legionella pneumophila strain ERS1305867

43.827

86.17

0.378
  pilT Acinetobacter baylyi ADP1

42.727

87.766

0.375
  pilT Pseudomonas aeruginosa PAK

42.683

87.234

0.372
  pilT Acinetobacter nosocomialis M2

42.901

86.17

0.37
  pilT Acinetobacter baumannii D1279779

42.901

86.17

0.37
  pilT Acinetobacter baumannii strain A118

42.901

86.17

0.37
  pilT Neisseria meningitidis 8013

40.896

89.096

0.364
  pilT Pseudomonas stutzeri DSM 10701

41.768

87.234

0.364