Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RY972_RS14105 Genome accession   NZ_CP136584
Coordinates   3054842..3055981 (+) Length   379 a.a.
NCBI ID   WP_005353124.1    Uniprot ID   A0A0T6QZZ8
Organism   Aeromonas allosaccharophila strain BIM B-1809     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3049842..3060981
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RY972_RS14075 (RY972_14075) - 3050065..3051129 (+) 1065 WP_317102323.1 3-deoxy-7-phosphoheptulonate synthase -
  RY972_RS14080 (RY972_14080) aac(6') 3051136..3051594 (+) 459 WP_317102324.1 aminoglycoside 6'-N-acetyltransferase -
  RY972_RS14085 (RY972_14085) - 3051617..3052414 (-) 798 WP_317104319.1 ATP-binding cassette domain-containing protein -
  RY972_RS14090 (RY972_14090) btuC 3052480..3053487 (-) 1008 WP_317102325.1 vitamin B12 ABC transporter permease BtuC -
  RY972_RS14095 (RY972_14095) - 3053583..3054314 (-) 732 WP_185900002.1 ABC transporter substrate-binding protein -
  RY972_RS14100 (RY972_14100) - 3054427..3054783 (+) 357 WP_005337656.1 DMT family protein -
  RY972_RS14105 (RY972_14105) pilU 3054842..3055981 (+) 1140 WP_005353124.1 type IVa pilus ATPase TapW Machinery gene
  RY972_RS14110 (RY972_14110) - 3056076..3056525 (-) 450 WP_317102326.1 isoprenylcysteine carboxylmethyltransferase family protein -
  RY972_RS14115 (RY972_14115) alr 3056699..3057925 (+) 1227 WP_317102327.1 alanine racemase -
  RY972_RS14120 (RY972_14120) - 3057991..3058935 (-) 945 WP_317102328.1 TerC family protein -
  RY972_RS14125 (RY972_14125) oppF 3059238..3060233 (-) 996 WP_042057772.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42253.34 Da        Isoelectric Point: 6.6011

>NTDB_id=891746 RY972_RS14105 WP_005353124.1 3054842..3055981(+) (pilU) [Aeromonas allosaccharophila strain BIM B-1809]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLNETPLEPGEVARIAAEIMDGEQKQQFEKDLEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKGDFF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=891746 RY972_RS14105 WP_005353124.1 3054842..3055981(+) (pilU) [Aeromonas allosaccharophila strain BIM B-1809]
ATGGAATTGCGAGACATGTTACAGATCCTGGCCAAGCAGGATGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
CGCCAAATTTAACGGCGGCCTGCGCCCCCTCAACGAAACGCCGCTGGAACCGGGTGAAGTGGCGCGGATCGCCGCCGAGA
TCATGGATGGCGAGCAGAAGCAGCAGTTCGAGAAGGATCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATCGGCCGC
TTTCGGGTCAACATCTTCAAGCAGCGCAACGAAGTCTCTCTGGTGGCCCGCAACATCAAGACCGAAATCCCCCGCTTTGA
AGATCTCAAACTCCCGCCTGTGCTGCTCGACACCATCATGGAGAAACGTGGCCTGGTGCTGTTTGTCGGCGGTACCGGCT
CGGGCAAATCCACTTCGCTGGCGGCGCTGATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAAGAT
CCGGTGGAGTTCGTTCATCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTTGGCGTGGATACCCGCAGCTTCCACGC
TGCCCTGAAAAACACCCTGCGTCAGGCGCCGGATGTCATTCTTATCGGCGAGATCCGCGATCGCGAGACCATGGAACATG
CGCTGGCCTTCTCCGAAACCGGCCACCTTGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGTCCGCAACTGCTCAACGATCTGGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
GCTGGTGAAAACCACCGATGGCGGCCGCCGGGCCGCAGTGGAGATCATGCTTGGCACCCACACCATCCGCGACATGATCA
AGCGCGGCGAATTTGGCGGCCTCAAAGAGGTGATGGAGAAATCCAAAGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTTGATCTGGTGGTGGAAGGGGTAATTGAGGAAGAAGAGGCGGTGAAAAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGACAGATTGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAACCCACCAAGGACG
AGAAGGGCGATTTTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6QZZ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.474

100

0.546
  pilU Acinetobacter baylyi ADP1

53.239

93.668

0.499
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

89.446

0.375
  pilT Legionella pneumophila strain ERS1305867

42.515

88.127

0.375
  pilT Legionella pneumophila strain Lp02

42.515

88.127

0.375
  pilT Pseudomonas aeruginosa PAK

41.279

90.765

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.698

90.765

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.742

87.863

0.367
  pilT Vibrio cholerae strain A1552

41.742

87.863

0.367
  pilT Acinetobacter baumannii strain A118

41.617

88.127

0.367
  pilT Acinetobacter baumannii D1279779

41.617

88.127

0.367
  pilT Acinetobacter nosocomialis M2

41.617

88.127

0.367