Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   Q9312_RS07020 Genome accession   NZ_CP133548
Coordinates   1581267..1581707 (+) Length   146 a.a.
NCBI ID   WP_309203878.1    Uniprot ID   A0AA51X876
Organism   Pleionea litopenaei strain HL-JVS1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1576267..1586707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q9312_RS07005 (Q9312_07005) ampD 1576684..1577283 (-) 600 WP_309203875.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  Q9312_RS07010 (Q9312_07010) - 1577386..1579632 (-) 2247 WP_309203876.1 DUF1631 domain-containing protein -
  Q9312_RS07015 (Q9312_07015) nadC 1580000..1580857 (+) 858 WP_309203877.1 carboxylating nicotinate-nucleotide diphosphorylase -
  Q9312_RS07020 (Q9312_07020) pilA2 1581267..1581707 (+) 441 WP_309203878.1 pilin Machinery gene
  Q9312_RS07025 (Q9312_07025) pilB 1581873..1583573 (+) 1701 WP_309203879.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q9312_RS07030 (Q9312_07030) pilC 1583634..1584827 (+) 1194 WP_309203880.1 type II secretion system F family protein Machinery gene
  Q9312_RS07035 (Q9312_07035) - 1584828..1585727 (+) 900 WP_309203881.1 A24 family peptidase -
  Q9312_RS07040 (Q9312_07040) coaE 1585739..1586338 (+) 600 WP_309203882.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 14693.88 Da        Isoelectric Point: 4.9499

>NTDB_id=874799 Q9312_RS07020 WP_309203878.1 1581267..1581707(+) (pilA2) [Pleionea litopenaei strain HL-JVS1]
MKKTNSGFTLIELMIVVAIIGILAAIALPAYQDYTIRSKVSEGLALGAEAKTAVAETAASLGGLANVTAANTGYTFPAGG
TDYVNSIAITDATGVITITMGGTQGTGATTEPVITLTPNQAAPEAPITWVCATTAGLPKHVPANCR

Nucleotide


Download         Length: 441 bp        

>NTDB_id=874799 Q9312_RS07020 WP_309203878.1 1581267..1581707(+) (pilA2) [Pleionea litopenaei strain HL-JVS1]
ATGAAAAAAACTAACTCAGGTTTTACCTTAATCGAATTAATGATCGTTGTTGCGATCATCGGTATTCTTGCAGCTATCGC
TCTTCCTGCGTACCAAGATTACACTATCCGTTCAAAAGTATCTGAAGGTTTAGCATTAGGTGCTGAAGCGAAAACTGCTG
TTGCAGAAACTGCTGCATCTTTAGGTGGTCTAGCTAACGTAACTGCAGCTAACACTGGTTATACTTTCCCAGCGGGTGGT
ACTGATTATGTAAACAGCATCGCTATCACTGACGCTACTGGCGTAATCACTATTACTATGGGTGGAACTCAAGGTACTGG
TGCAACAACTGAGCCAGTCATCACTTTGACTCCTAACCAAGCAGCTCCAGAAGCGCCAATCACTTGGGTTTGTGCGACTA
CTGCTGGTCTTCCAAAGCACGTTCCAGCTAACTGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-146)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

54.93

97.26

0.534
  pilA2 Legionella pneumophila str. Paris

54.93

97.26

0.534
  pilA Ralstonia pseudosolanacearum GMI1000

40.782

100

0.5
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.095

100

0.493
  comP Acinetobacter baylyi ADP1

43.791

100

0.459
  pilA/pilA1 Eikenella corrodens VA1

41.026

100

0.438
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.958

97.945

0.411
  pilA Haemophilus influenzae Rd KW20

40.816

100

0.411
  pilE Neisseria gonorrhoeae strain FA1090

35.583

100

0.397
  pilE Neisseria gonorrhoeae MS11

34.132

100

0.39
  pilA Haemophilus influenzae 86-028NP

38.732

97.26

0.377