Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q9312_RS07025 Genome accession   NZ_CP133548
Coordinates   1581873..1583573 (+) Length   566 a.a.
NCBI ID   WP_309203879.1    Uniprot ID   A0AA51X904
Organism   Pleionea litopenaei strain HL-JVS1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1576873..1588573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q9312_RS07010 (Q9312_07010) - 1577386..1579632 (-) 2247 WP_309203876.1 DUF1631 domain-containing protein -
  Q9312_RS07015 (Q9312_07015) nadC 1580000..1580857 (+) 858 WP_309203877.1 carboxylating nicotinate-nucleotide diphosphorylase -
  Q9312_RS07020 (Q9312_07020) pilA2 1581267..1581707 (+) 441 WP_309203878.1 pilin Machinery gene
  Q9312_RS07025 (Q9312_07025) pilB 1581873..1583573 (+) 1701 WP_309203879.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q9312_RS07030 (Q9312_07030) pilC 1583634..1584827 (+) 1194 WP_309203880.1 type II secretion system F family protein Machinery gene
  Q9312_RS07035 (Q9312_07035) - 1584828..1585727 (+) 900 WP_309203881.1 A24 family peptidase -
  Q9312_RS07040 (Q9312_07040) coaE 1585739..1586338 (+) 600 WP_309203882.1 dephospho-CoA kinase -
  Q9312_RS07045 (Q9312_07045) zapD 1586342..1587094 (+) 753 WP_309203884.1 cell division protein ZapD -
  Q9312_RS07050 (Q9312_07050) - 1587193..1587381 (+) 189 WP_309203885.1 DNA gyrase inhibitor YacG -
  Q9312_RS07055 (Q9312_07055) mutT 1587427..1587819 (-) 393 WP_309203886.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62247.20 Da        Isoelectric Point: 4.8118

>NTDB_id=874800 Q9312_RS07025 WP_309203879.1 1581873..1583573(+) (pilB) [Pleionea litopenaei strain HL-JVS1]
MSGTFVSGLTRRLVNEGLISETQAAEAGQAAQKTSTPIVKYLIDNQIIPSDVLVNLASVEFGVPIFDLSSLDYDQIPKGV
VDEKLIAKHQATPIFKRGNRLFVAVSDPTNLIALDEIQFHSKLTVEAVLVEQKKLEPLIDKIIEEQDSQNLAEFDDADLE
NLDVESGDTKPADDDDASGDDAPIVRFVNKILLDAIKKGASDLHFEPYEKNYRVRFRIDGILHEVSSPPVNLSTRISARL
KVMSRLDISEKRKPQDGRIKLKLSKNKAIDFRVSTLPTLFGEKIVMRILDPSSAMLGIEVLGYEPEQRKLYEECLFKPQG
MVLVTGPTGSGKTVSLYTGVNMLNQPERNISTAEDPVEINLSGINQVNVNPKAGLDFSSALRAFLRQDPDVILVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLVNMGVAPFNIATSVNLVIAQRLARRLCSHCKRVIDIPKEAQLELGFTE
EQIGTFEIYEPVGCDNCTQGYKGRVGIYQVMPVSETMGRIIMEGGNAIDIADQAAKEGVNDLRKSGLLKVIQGVTSLEEV
NRVTQD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=874800 Q9312_RS07025 WP_309203879.1 1581873..1583573(+) (pilB) [Pleionea litopenaei strain HL-JVS1]
ATGTCTGGAACTTTTGTTAGTGGACTCACCCGACGCTTAGTCAATGAAGGCCTCATCAGCGAAACACAAGCAGCCGAGGC
CGGACAAGCTGCGCAAAAAACATCGACGCCAATCGTCAAGTATTTGATTGATAATCAAATAATTCCATCAGATGTTCTGG
TTAACTTGGCTTCCGTTGAATTCGGTGTACCTATTTTCGACCTAAGTTCACTCGATTATGACCAAATTCCCAAAGGCGTA
GTCGATGAGAAGCTTATCGCCAAGCATCAAGCAACCCCTATTTTCAAACGTGGAAATCGCTTATTCGTTGCTGTTTCAGA
CCCGACCAACTTAATCGCCCTTGATGAAATTCAGTTTCACAGCAAACTGACCGTTGAGGCCGTTCTAGTCGAGCAGAAAA
AGCTCGAGCCGCTGATTGATAAAATCATTGAAGAACAAGACAGTCAAAACCTTGCTGAGTTTGACGACGCCGATCTCGAA
AACCTCGATGTTGAAAGCGGAGACACTAAACCAGCCGACGACGACGATGCTAGCGGTGACGATGCGCCTATTGTTCGTTT
CGTGAATAAGATTTTACTCGATGCGATTAAAAAAGGCGCTTCAGATTTACACTTCGAACCCTACGAGAAAAACTATCGCG
TCCGCTTTCGTATCGATGGCATCTTACATGAAGTGTCTAGCCCTCCAGTAAATCTGTCGACCCGCATTTCAGCACGTTTA
AAAGTCATGTCTCGACTCGACATTTCAGAAAAGCGAAAACCACAAGATGGTCGGATTAAGTTAAAACTGTCAAAGAACAA
AGCCATCGACTTTCGTGTTAGCACTCTGCCAACACTTTTCGGCGAAAAAATCGTTATGCGTATACTCGACCCTTCGAGCG
CCATGTTAGGTATCGAAGTGTTAGGCTATGAGCCGGAGCAACGCAAACTGTATGAAGAATGTCTATTTAAGCCTCAAGGC
ATGGTCTTGGTTACCGGTCCGACAGGTTCGGGTAAAACGGTTTCTCTTTACACGGGTGTTAACATGCTCAACCAACCCGA
GCGAAATATTTCGACCGCAGAAGACCCGGTTGAGATTAACTTGTCAGGGATCAATCAGGTCAATGTAAACCCCAAGGCTG
GATTAGATTTCTCCTCTGCCCTTCGAGCTTTCCTTCGACAAGATCCTGACGTTATTCTGGTTGGTGAGATTCGTGACCTA
GAAACTGCAGAAATCGCCATCAAAGCCGCACAAACGGGTCATATGGTACTGTCCACTTTGCACACCAACAGCGCTCCAGA
AACCTTGACTCGTCTGGTCAATATGGGCGTTGCACCTTTCAATATCGCAACGAGTGTCAATCTAGTTATTGCACAGCGAT
TAGCACGAAGATTGTGCAGCCATTGTAAACGGGTCATCGATATTCCCAAAGAAGCACAATTAGAGCTGGGATTTACGGAA
GAACAAATAGGAACTTTCGAAATTTATGAACCAGTTGGTTGTGATAATTGTACGCAAGGGTATAAAGGACGTGTTGGTAT
TTATCAAGTAATGCCCGTCTCTGAAACCATGGGTCGGATCATTATGGAAGGTGGCAATGCCATCGATATTGCTGATCAAG
CAGCAAAAGAAGGCGTAAACGACTTGAGAAAGTCAGGTTTACTCAAGGTTATTCAAGGGGTCACTTCTCTCGAAGAGGTC
AACCGCGTAACACAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(13-148)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.879

99.647

0.617
  pilB Acinetobacter baylyi ADP1

61.279

99.47

0.61
  pilB Legionella pneumophila strain ERS1305867

56.184

100

0.562
  pilF Neisseria gonorrhoeae MS11

51.51

99.47

0.512
  pilB Vibrio cholerae strain A1552

51.906

97.35

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

50.178

99.293

0.498
  pilB Vibrio campbellii strain DS40M4

48.677

100

0.488
  pilF Thermus thermophilus HB27

37.658

98.057

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.008

86.219

0.362
  pilB/pilB1 Synechocystis sp. PCC 6803

40.8

88.339

0.36