Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   RDV54_RS08010 Genome accession   NZ_CP133471
Coordinates   1686515..1687456 (+) Length   313 a.a.
NCBI ID   WP_000249545.1    Uniprot ID   -
Organism   Streptococcus mitis strain LMG 14557     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1681515..1692456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RDV54_RS07990 (RDV54_07990) - 1681583..1683400 (+) 1818 WP_001220872.1 acyltransferase family protein -
  RDV54_RS07995 (RDV54_07995) nagA 1683552..1684703 (+) 1152 WP_001134449.1 N-acetylglucosamine-6-phosphate deacetylase -
  RDV54_RS08000 (RDV54_08000) - 1684866..1685924 (+) 1059 WP_000649460.1 zinc-dependent alcohol dehydrogenase family protein -
  RDV54_RS08005 (RDV54_08005) - 1686074..1686439 (+) 366 WP_000286405.1 DUF1033 family protein -
  RDV54_RS08010 (RDV54_08010) comGA/cglA/cilD 1686515..1687456 (+) 942 WP_000249545.1 competence type IV pilus ATPase ComGA Machinery gene
  RDV54_RS08015 (RDV54_08015) comGB/cglB 1687404..1688420 (+) 1017 WP_004238896.1 competence type IV pilus assembly protein ComGB Machinery gene
  RDV54_RS08020 (RDV54_08020) comGC/cglC 1688422..1688745 (+) 324 WP_000738641.1 comG operon protein ComGC Machinery gene
  RDV54_RS08025 (RDV54_08025) comGD/cglD 1688708..1689142 (+) 435 WP_029689647.1 competence type IV pilus minor pilin ComGD Machinery gene
  RDV54_RS08030 (RDV54_08030) comGE/cglE 1689105..1689407 (+) 303 WP_045597458.1 competence type IV pilus minor pilin ComGE Machinery gene
  RDV54_RS08035 (RDV54_08035) comGF/cglF 1689370..1689831 (+) 462 WP_000250509.1 competence type IV pilus minor pilin ComGF Machinery gene
  RDV54_RS08040 (RDV54_08040) comGG/cglG 1689809..1690222 (+) 414 WP_000265657.1 competence type IV pilus minor pilin ComGG Machinery gene
  RDV54_RS08045 (RDV54_08045) - 1690255..1690842 (+) 588 WP_000679792.1 class I SAM-dependent methyltransferase -
  RDV54_RS08050 (RDV54_08050) comYH 1690903..1691856 (+) 954 WP_000345104.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35537.48 Da        Isoelectric Point: 6.0063

>NTDB_id=874514 RDV54_RS08010 WP_000249545.1 1686515..1687456(+) (comGA/cglA/cilD) [Streptococcus mitis strain LMG 14557]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGCYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYE
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQDNMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANKDYQEHQSTSWNAQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=874514 RDV54_RS08010 WP_000249545.1 1686515..1687456(+) (comGA/cglA/cilD) [Streptococcus mitis strain LMG 14557]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGGGCGCAGGATATTTATTTTGTTCCTAAGTT
AGACGCTTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATCGGTTGTTATGATTTTGAAAAATTTGCGGCCG
TCATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTTGGAGAAAAGCGACGTAGTCAGCTTGGTTCCTGTGACTATGAA
TATGACCAGAAGATGGCGTCCTTACGGTTATCTACTGTAGGCGACTATCGAGGGCATGAGAGTTTAGTTATTCGCTTGTT
GCATGATGAGGAGCAGGATTTGCATTTTTGGTTTCAGGATATTGAAGAACTGGGCAAGCAGTACAGGCAACGGGGACTTT
ATCTTTTTGCTGGTCCAGTCGGAAGTGGCAAGACAACCTTGATGCATGAATTGGCTAAATCTCTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTAGAAATCAAGCAGGACAACATGCTCCAGTTGCAGTTGAACGAAGCAATCGGGTTGAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGTCCTGATCTCTTGATTATCGGAGAAATTCGGGACAGCGAGACAG
CGCGTGCAGTGGTCAGAGCCAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCATGCCAAGAGTATCCGAGGAGTTTAT
GAACGTTTACTGGAGTTGGGCGTGAGTGAGGAGGAATTGGCAGTCGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAAAGACTATCAAGAACACCAGTCAACTAGCTGGAATGCGCAGATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

100

100

1
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

95.847

100

0.958
  comGA/cglA/cilD Streptococcus pneumoniae D39

95.847

100

0.958
  comGA/cglA/cilD Streptococcus pneumoniae R6

95.847

100

0.958
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

95.847

100

0.958
  comYA Streptococcus gordonii str. Challis substr. CH1

78.387

99.042

0.776
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.258

99.042

0.617
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

43.019

84.665

0.364