NTDB

Overview

Name	comGB/cglB	Type	Machinery gene
Locus tag	RDV54_RS08015	Genome accession	NZ_CP133471
Coordinates	1687404..1688420 (+)	Length	338 a.a.
NCBI ID	WP_004238896.1	Uniprot ID	-
Organism	Streptococcus mitis strain LMG 14557
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1682404..1693420

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RDV54_RS07995 (RDV54_07995)	nagA	1683552..1684703 (+)	1152	WP_001134449.1	N-acetylglucosamine-6-phosphate deacetylase	-
RDV54_RS08000 (RDV54_08000)	-	1684866..1685924 (+)	1059	WP_000649460.1	zinc-dependent alcohol dehydrogenase family protein	-
RDV54_RS08005 (RDV54_08005)	-	1686074..1686439 (+)	366	WP_000286405.1	DUF1033 family protein	-
RDV54_RS08010 (RDV54_08010)	comGA/cglA/cilD	1686515..1687456 (+)	942	WP_000249545.1	competence type IV pilus ATPase ComGA	Machinery gene
RDV54_RS08015 (RDV54_08015)	comGB/cglB	1687404..1688420 (+)	1017	WP_004238896.1	competence type IV pilus assembly protein ComGB	Machinery gene
RDV54_RS08020 (RDV54_08020)	comGC/cglC	1688422..1688745 (+)	324	WP_000738641.1	comG operon protein ComGC	Machinery gene
RDV54_RS08025 (RDV54_08025)	comGD/cglD	1688708..1689142 (+)	435	WP_029689647.1	competence type IV pilus minor pilin ComGD	Machinery gene
RDV54_RS08030 (RDV54_08030)	comGE/cglE	1689105..1689407 (+)	303	WP_045597458.1	competence type IV pilus minor pilin ComGE	Machinery gene
RDV54_RS08035 (RDV54_08035)	comGF/cglF	1689370..1689831 (+)	462	WP_000250509.1	competence type IV pilus minor pilin ComGF	Machinery gene
RDV54_RS08040 (RDV54_08040)	comGG/cglG	1689809..1690222 (+)	414	WP_000265657.1	competence type IV pilus minor pilin ComGG	Machinery gene
RDV54_RS08045 (RDV54_08045)	-	1690255..1690842 (+)	588	WP_000679792.1	class I SAM-dependent methyltransferase	-
RDV54_RS08050 (RDV54_08050)	comYH	1690903..1691856 (+)	954	WP_000345104.1	class I SAM-dependent methyltransferase	Machinery gene
RDV54_RS08055 (RDV54_08055)	-	1691906..1693096 (+)	1191	WP_000167778.1	acetate kinase	-

Sequence

Protein

Download Length: 338 a.a. Molecular weight: 38362.31 Da Isoelectric Point: 9.4588

                                >NTDB_id=874515 RDV54_RS08015 WP_004238896.1 1687404..1688420(+) (comGB/cglB) [Streptococcus mitis strain LMG 14557]

MDISQVFRLKRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRAGLSQGKSFSEMMESLGFSSAI

VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF

LGMVGFVSVGVLLALTFYKRGSKMRVFSILARIPFLGIFVQTYLTAYYAREWGNMISQGMELTQIFQIMQEQGSQLFKEI

GQDMAHALQNGQEFSQTIGTYPFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV

LLYAAMLMPMYQNMEVNF

Nucleotide

Download Length: 1017 bp

                                >NTDB_id=874515 RDV54_RS08015 WP_004238896.1 1687404..1688420(+) (comGB/cglB) [Streptococcus mitis strain LMG 14557]

ATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCTTGTTTAA

TAATCTCTTTTCCAGCGGTTTTCATCTGGTAGAGACTATCTCCTTTCTAGATAGGAGTGCCTTGTTGGACAAGCAGTGCG

TAACCCAGATGCGTGCGGGCTTATCTCAAGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGCTTTTCAAGTGCCATT

GTCACTCAGTTATCCCTAGCAGAGGTTCATGGGAATCTACACCTGAGTTTGGGAAAGATAGAAGAATATCTGGATAATTT

GGCCAAGGTCAAGAAAAAACTAATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGGTTTCTTCTCTTAATTATGCTGG

GGTTGCGTAACTACCTACTACCACAACTGGATAGTAGCAATATTGCCACCCAAATTATTGGCAATCTGCCACAAATTTTT

CTAGGAATGGTAGGCTTTGTTTCAGTAGGTGTCCTTTTAGCACTCACTTTTTATAAAAGAGGTTCGAAGATGCGGGTCTT

TTCTATCCTGGCACGCATTCCTTTTCTTGGAATTTTTGTGCAGACCTATCTGACAGCCTATTATGCGCGTGAATGGGGCA

ATATGATTTCTCAAGGGATGGAGTTGACGCAGATTTTTCAAATCATGCAGGAACAAGGTTCCCAGCTATTTAAAGAAATT

GGTCAAGATATGGCTCATGCCCTACAAAATGGTCAGGAATTTTCTCAAACCATAGGAACCTATCCTTTCTTTAAAAAGGA

GTTGAGTCTCATCATCGAGTATGGGGAAGTCAAGTCCAAGTTGGGGAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG

AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATCTGGTGCAGCCACTGGTCTTTATCTTTGTGGCTCTGATTATCGTT

TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGB/cglB	Streptococcus mitis NCTC 12261	100	100	1
comGB/cglB	Streptococcus mitis SK321	96.154	100	0.962
comGB/cglB	Streptococcus pneumoniae Rx1	94.675	100	0.947
comGB/cglB	Streptococcus pneumoniae D39	94.675	100	0.947
comGB/cglB	Streptococcus pneumoniae R6	94.675	100	0.947
comGB/cglB	Streptococcus pneumoniae TIGR4	94.675	100	0.947
comYB	Streptococcus gordonii str. Challis substr. CH1	72.321	99.408	0.719
comYB	Streptococcus mutans UA140	59.248	94.379	0.559
comYB	Streptococcus mutans UA159	59.248	94.379	0.559
comGB	Lactococcus lactis subsp. cremoris KW2	50.599	98.817	0.5

Detailed information NTG0874515