Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RAM80_RS26460 Genome accession   NZ_CP133165
Coordinates   5737361..5738515 (+) Length   384 a.a.
NCBI ID   WP_416423342.1    Uniprot ID   -
Organism   Pseudomonas sp. App30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5732361..5743515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM80_RS26435 (RAM80_26355) - 5732472..5732879 (-) 408 WP_416423338.1 hypothetical protein -
  RAM80_RS26440 (RAM80_26360) - 5733076..5733471 (+) 396 WP_416423339.1 RidA family protein -
  RAM80_RS26445 (RAM80_26365) - 5733503..5734702 (+) 1200 WP_416423340.1 amino acid deaminase -
  RAM80_RS26450 (RAM80_26370) - 5734772..5735047 (-) 276 WP_008369722.1 peptidylprolyl isomerase -
  RAM80_RS26455 (RAM80_26375) - 5735099..5737273 (-) 2175 WP_416423341.1 PAS domain-containing protein -
  RAM80_RS26460 (RAM80_26380) pilU 5737361..5738515 (+) 1155 WP_416423342.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RAM80_RS26465 (RAM80_26385) hbdH 5738663..5739433 (-) 771 WP_416423343.1 3-hydroxybutyrate dehydrogenase -
  RAM80_RS26470 (RAM80_26390) - 5739450..5741036 (-) 1587 WP_416427567.1 ABC transporter ATP-binding protein -
  RAM80_RS26475 (RAM80_26395) - 5741044..5742066 (-) 1023 WP_416423344.1 ABC transporter permease -
  RAM80_RS26480 (RAM80_26400) - 5742066..5743127 (-) 1062 WP_416423345.1 microcin C ABC transporter permease YejB -

Sequence

Protein


Download         Length: 384 a.a.        Molecular weight: 42602.54 Da        Isoelectric Point: 5.8400

>NTDB_id=872270 RAM80_RS26460 WP_416423342.1 5737361..5738515(+) (pilU) [Pseudomonas sp. App30]
MEIQALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLSAEALKPGEVAAIAADIMDAEQRQDFERDLEMNLAMSIHGVGR
FRVNIFKQRNEVSIVARNIKLDIPRFEDLKLPPVLLETIMQKRGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFQAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLGNNLQAFVSQRLVKTTDGKRRAAVEVMLGTATIKDLIHRNEFGELKGIMEKSVNLGMKTFDQAL
YELALEGAISEEEALKNADSQNNLRLRLKLHRENGHEPSSVPLPPEPAADVDSADWNLVDDENT

Nucleotide


Download         Length: 1155 bp        

>NTDB_id=872270 RAM80_RS26460 WP_416423342.1 5737361..5738515(+) (pilU) [Pseudomonas sp. App30]
ATGGAAATCCAAGCCCTGCTGAAAATCCTCGCCTCCCAGGACGGTTCCGATCTCTACCTGTCCACCGGGGCGCCGCCCTG
CGCCAAGTTCAACGGCGTGCTCAAGCCACTGTCGGCGGAAGCGCTGAAACCCGGCGAAGTGGCGGCCATCGCCGCCGATA
TCATGGACGCCGAACAGCGCCAGGATTTCGAGCGTGACCTGGAGATGAACCTGGCCATGTCCATTCATGGCGTCGGACGC
TTCCGGGTGAATATCTTCAAACAGCGCAACGAAGTGTCCATCGTCGCCCGTAACATCAAGCTCGACATTCCCCGTTTCGA
AGACCTCAAGCTGCCGCCGGTGCTGCTCGAGACCATCATGCAGAAGCGCGGCCTGGTGCTGTTCGTCGGCGCCACCGGCT
CGGGCAAGTCGACTTCCTTGGCGGCGCTGATCGACTATCGCAACCGCAACGCCAGCGGCCACATCATCACCATCGAAGAC
CCGGTGGAGTATGTGCACCGGCACAAGAAGTCGATCATCAACCAGCGCGAGGTGGGTGTCGACACCCGCAGCTTCCAGGC
GGCCTTGAAGAACACCTTGCGCCAGGCGCCGGATGTCATCCTGATCGGCGAAATTCGCGACCGCGAGACCATGGAGCACG
CCCTGGCGTTCGCCGATACCGGCCATCTGGCCATCTCGACGCTGCACGCCAACAACGCCAACCAGGCGCTCGACCGCATC
ATCAACTTCTTCCCCGAGGACCGTCGCCCGCAGTTGCTCAATGACCTGGGCAACAACCTGCAGGCGTTCGTCTCCCAGCG
GCTGGTGAAGACCACCGACGGTAAGCGCCGGGCGGCCGTGGAGGTGATGCTGGGCACCGCCACCATCAAGGACCTGATTC
ACCGCAACGAGTTCGGCGAGCTCAAGGGCATCATGGAAAAATCGGTGAACCTGGGGATGAAGACGTTTGACCAGGCGCTG
TACGAATTGGCCCTGGAGGGGGCGATCAGCGAGGAGGAAGCACTGAAGAACGCCGACTCGCAGAACAACCTGCGCCTGCG
CCTCAAGCTGCACCGTGAAAACGGCCATGAGCCCAGCAGCGTGCCACTGCCGCCGGAGCCGGCGGCGGACGTGGACAGCG
CTGACTGGAACCTGGTGGATGATGAGAACACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.474

94.531

0.534
  pilU Acinetobacter baylyi ADP1

52.113

92.448

0.482
  pilU Vibrio cholerae strain A1552

50.276

94.271

0.474
  pilT Pseudomonas stutzeri DSM 10701

41.86

89.583

0.375
  pilT Pseudomonas aeruginosa PAK

41.57

89.583

0.372
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.766

97.917

0.37
  pilT Legionella pneumophila strain Lp02

42.515

86.979

0.37
  pilT Legionella pneumophila strain ERS1305867

42.515

86.979

0.37
  pilT Acinetobacter baumannii strain A118

41.916

86.979

0.365
  pilT Acinetobacter nosocomialis M2

41.916

86.979

0.365
  pilT Acinetobacter baumannii D1279779

41.916

86.979

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.828

90.885

0.362
  pilT Vibrio cholerae strain A1552

39.828

90.885

0.362