Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RBH93_RS07830 Genome accession   NZ_CP133079
Coordinates   1661488..1662627 (-) Length   379 a.a.
NCBI ID   WP_005353124.1    Uniprot ID   A0A0T6QZZ8
Organism   Aeromonas veronii strain HS1906     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1656488..1667627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RBH93_RS07810 (RBH93_07810) oppF 1657235..1658230 (+) 996 WP_005337666.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -
  RBH93_RS07815 (RBH93_07815) - 1658533..1659477 (+) 945 WP_005337664.1 TerC family protein -
  RBH93_RS07820 (RBH93_07820) alr 1659543..1660769 (-) 1227 WP_307896510.1 alanine racemase -
  RBH93_RS07825 (RBH93_07825) - 1660943..1661392 (+) 450 WP_307896511.1 isoprenylcysteine carboxylmethyltransferase family protein -
  RBH93_RS07830 (RBH93_07830) pilU 1661488..1662627 (-) 1140 WP_005353124.1 type IVa pilus ATPase TapW Machinery gene
  RBH93_RS07835 (RBH93_07835) - 1662686..1663042 (-) 357 WP_005337656.1 DMT family protein -
  RBH93_RS07840 (RBH93_07840) - 1663155..1663886 (+) 732 WP_307896512.1 ABC transporter substrate-binding protein -
  RBH93_RS07845 (RBH93_07845) btuC 1663983..1664990 (+) 1008 WP_307896513.1 vitamin B12 ABC transporter permease BtuC -
  RBH93_RS07850 (RBH93_07850) - 1665056..1665853 (+) 798 WP_307896833.1 ATP-binding cassette domain-containing protein -
  RBH93_RS07855 (RBH93_07855) aac(6') 1665876..1666334 (-) 459 WP_139464053.1 aminoglycoside 6'-N-acetyltransferase -
  RBH93_RS07860 (RBH93_07860) - 1666341..1667405 (-) 1065 WP_307896514.1 3-deoxy-7-phosphoheptulonate synthase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42253.34 Da        Isoelectric Point: 6.6011

>NTDB_id=871664 RBH93_RS07830 WP_005353124.1 1661488..1662627(-) (pilU) [Aeromonas veronii strain HS1906]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLNETPLEPGEVARIAAEIMDGEQKQQFEKDLEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKGDFF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=871664 RBH93_RS07830 WP_005353124.1 1661488..1662627(-) (pilU) [Aeromonas veronii strain HS1906]
ATGGAATTGCGAGACATGTTACAGATCCTGGCCAAGCAGGATGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
CGCCAAATTTAACGGCGGCCTGCGCCCCCTCAACGAAACGCCGCTGGAACCGGGTGAAGTGGCGCGGATCGCCGCCGAGA
TCATGGATGGCGAGCAGAAGCAGCAGTTCGAGAAGGATCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATCGGCCGC
TTTCGGGTCAACATCTTCAAGCAGCGCAACGAAGTCTCTCTGGTGGCCCGCAACATCAAGACCGAAATCCCCCGCTTTGA
AGATCTCAAACTCCCGCCTGTGTTGCTCGACACCATCATGGAGAAACGCGGTCTGGTGCTGTTTGTCGGCGGTACCGGCT
CGGGCAAATCCACTTCGCTGGCGGCGCTTATCGACCACCGCAACCGCAACAGCGGTGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTTCATCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTTGGCGTGGATACCCGCAGCTTCCACGC
AGCCCTGAAAAACACCCTGCGTCAGGCGCCGGACGTCATTCTTATCGGCGAGATCCGCGATCGCGAGACCATGGAACATG
CGCTGGCCTTCTCCGAAACCGGCCACCTTGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGCCCCCAGCTGCTCAACGATCTGGGCAACAACCTCAAGGCTTTTGTCTCCCAGCG
GCTGGTGAAAACCACCGATGGCGGTCGCCGGGCCGCAGTGGAGATCATGCTTGGCACCCACACCATCCGCGACATGATCA
AGCGCGGCGAATTTGGTGGTCTCAAAGAGGTGATGGAGAAATCCAAGGCCCTCGGCATGGTTACCTTCGACAGTGCCCTG
TTTGATCTGGTGGTGGAAGGGGTAATTGAGGAAGAAGAGGCGGTGAAAAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGTTGTGGAAGGAGAAGGGGCAGATTGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAACCCACCAAGGACG
AGAAGGGCGATTTTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6QZZ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.474

100

0.546
  pilU Acinetobacter baylyi ADP1

53.239

93.668

0.499
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

89.446

0.375
  pilT Legionella pneumophila strain ERS1305867

42.515

88.127

0.375
  pilT Legionella pneumophila strain Lp02

42.515

88.127

0.375
  pilT Pseudomonas aeruginosa PAK

41.279

90.765

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.698

90.765

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.742

87.863

0.367
  pilT Vibrio cholerae strain A1552

41.742

87.863

0.367
  pilT Acinetobacter baumannii strain A118

41.617

88.127

0.367
  pilT Acinetobacter baumannii D1279779

41.617

88.127

0.367
  pilT Acinetobacter nosocomialis M2

41.617

88.127

0.367